Ligand 2-nitro-5-[(phenylacetyl)amino]-benzoic acid

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Basic Ligand Information

Molecular Structure
Picture of 2-nitro-5-[(phenylacetyl)amino]-benzoic acid (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C15H12N2O5
2-nitro-5-[(phenylacetyl)amino]-benzoic acid
QHVQEQRGDKOHHC-UHFFFAOYSA-N
Synonyms:
2-nitro-5-phenylacetamidobenzoic acid, 2-nitro-5-[(phenyl-acetyl)amino]benzoic acid, 2-nitro-5-[(phenylacetyl)amino]benzoic acid, 6-nitro-3-(phenylacetamido)-benzoic acid, 6-nitro-3-(phenylacetamido)benzoic acid, 6-nitro-3-phenylacetamide benzoic acid, 6-nitro-3-phenylacetamido-benzoic acid, 6-nitro-3-phenylacetamidobenzoic acid, 6-nitro-3-[(phenylacetyl)amino]benzoic acid, D-2-nitro-5-[(phenylacetyl)amino]benzoic acid, D-2-nitro-5-[(phenylglycyl)amino]benzoic acid, D-2-nitro-5[(phenylglycyl)amino]benzoic acid, nitrophenylacetylaminobenzoic acid

Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (18 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (20 results)

COMMENTARY
EC NUMBER
LITERATURE
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
30°C, pH 6.2
0.4
-
pH 9.1, 25°C, ionic strength 0.12 M
26
-
25°C, pH 7.0, 10% dimethylsulfoxide
20
-
pH 8.0, 37°C
68.8
-
pH 8.0, 25°C, ionic strength 0.12 M
27
-
pH 7.5, 25°C, enzyme form PA5..5
77
-
pH 7.5, 25°C, enzyme form PA5..3
82
-
pH 7.5, 25°C
20
-
pH 7.4, 37°C, recombinant enzyme
58.3
-
pH 7.0, 30°C, soluble enzyme
17.6
-
pH 7.0, 30°C, poly-lysine supported cross-linked enzyme aggregates
145.3
-
pH 7.0, 30°C, cross-linked enzyme aggregates
15.7
-
pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma
pH 7.0, 25°C, wild-type enzyme
15
-
pH 7.0, 25°C, mutant N241S
0.204
-
pH 7.0, 25°C, mutant N241G
0.354
-
pH 10.0, 25°C, ionic strength 0.12 M
16
-

KM Value (28 results)

COMMENTARY
EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
LITERATURE
30°C, pH 6.2
1.1
-
wild type protein, pH 7.5, 25°C
0.026
-
25°C, pH 7.0, 10% dimethylsulfoxide
0.000015
-
pH and temperature not specified in the publication
0.0197
-
pH 8.0, 37°C
0.027
-
pH 7.5, 37°C
0.0176
-
pH 7.5, 25°C, enzyme form PA5.5
0.017
-
pH 7.5, 25°C, enzyme form PA5.3
0.012
-
pH 7.5, 25°C
pH 7.4, 37°C, recombinant enzyme
0.0083
-
pH 7.0, 30°C, soluble enzyme
0.9919
-
pH 7.0, 30°C, poly-lysine supported cross-linked enzyme aggregates
7.9
-
pH 7.0, 30°C, cross-linked enzyme aggregates
30.39
-
pH 7.0, 30°C
pH 7.0, 25°C, mutant N241S
0.0102
-
pH 7.0, 25°C, mutant N241G
0.00614
-
pH 7.0, 25°C
0.03
-
betaF24S mutant protein, pH 7.5, 25°C
0.551
-
betaF24P mutant protein, pH 7.5, 25°C
0.797
-
betaF24A mutant protein, pH 7.5, 25°C
0.098
-
alphaF146Y mutant protein, pH 7.5, 25°C
0.0005
-
alphaF146R mutant protein, pH 7.5, 25°C
0.386
-
alphaF146Q mutant protein, pH 7.5, 25°C
0.272
-
alphaF146A mutant protein, pH 7.5, 25°C
0.116
-

References & Links

Links to other databases for 2-nitro-5-[(phenylacetyl)amino]-benzoic acid

ChEBI
PubChem
-
PubChem