Ligand dithioerythritol

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Basic Ligand Information

Molecular Structure
Picture of dithioerythritol (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C4H10O2S2
dithioerythritol
VHJLVAABSRFDPM-ZXZARUISSA-N
Synonyms:
1,4-dithioerythritol, dithioerythreitol, DTE

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
thiosulfate + 2 dithioerythritol = sulfite + dithioerythritol disulfide + sulfide
show the reaction diagram
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Substrate in Enzyme-catalyzed Reactions (19 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
ribonucleoside triphosphate + dithioerythritol = 2'-deoxyribonucleoside triphosphate + ? + H2O
show the reaction diagram
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N-methylglycine + phosphate + dithioerythritol = acetyl phosphate + methylamine + dithioerythritol disulfide
show the reaction diagram
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N,N,N-trimethylglycine + dithioerythritol = ?
show the reaction diagram
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dithioerythritol + O2 = ? + H2O
show the reaction diagram
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3'-phosphoadenylylsulfate + dithioerythritol = ?
show the reaction diagram
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L-Cysteine + dithioerythritol = ?
show the reaction diagram
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Enzyme Cofactor/Cosubstrate (7 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE

Activator in Enzyme-catalyzed Reactions (40 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
1 mM, slight activation
-
recovery from oxidase to dehydrogenase type
-
0.5 mM, maximal activation, 94% of activity with dithiothreitol
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equally effective as DTT
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absolutely required, highly stimulating
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can serve as artificial electron donor
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activates
-
10-20 mM, up to 39% inhibition
-
required for maximal activity
-
5 mM, activates
-
slight stimulation
-
5 mM, stimulation of 96%
-
10 mM, activates
-
stimulates enzyme activity up to 35%, optimum concentration: 6 mM
-
slight stimulation
-
2 mM, 200% activation
-
highly stimulating
-
maximal activation at 0.005 mM, inhibition above
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in combination wit Mg2+
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20% activation at 1 mM
-
1 mM, slight activation
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presence decreases extent of inactivation by Hg2+, Ag2+, thimerosal or p-hydroxymercuribenzoate
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activates, synergistic with EDTA
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with palmitoylaspartate as substrate
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effectively reverses heavy metal inactivation
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1-13 mM, activates
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increase of activity
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4 mM, enhances the enzyme activity about threefold. The addition of both 4 mM dithioerythritol and 4 mM Mn2+ enhances the enzyme activity approximately 10fold compared to the results of an assay lacking dithioerythritol and Mn2+
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increased catalytic activity with 10 mM
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GSH, dithiothreitol or dithioerythritol are required for activity
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Inhibitor in Enzyme-catalyzed Reactions (49 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
competitive
-
protection at low concentration, inhibition at high concentration, 100 mM
-
1.09 mM, 50% inhibition, partially reversed by diamide
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10 mM
-
2 mM, total activity loss
-
0.05 mM, 82% inhibition of catechol oxidation, 95% inhibition of pyrogallol oxidation and 62% inhibition of dopa oxidation
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50% inhibition at 4 mM
-
6.6 mM, 85% inhibition
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up to 0.010 mM increase the enzyme activity, higher concentrations inhibited it
-
up to 0.010 mM increase the enzyme activity, higher concentrations inhibit
-
52% inhibition at 2 mM
-
above 20 mM
-
10 mM, 83% activity retains
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13% inhibition with 13 mM
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concentration of 2.5 mM decreases activity of the 3 enzyme forms in colonic mucosa by 20% at pH 4.2 and by 25% at pH 7.0, similar results with the 2 forms from colon tumors
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formation of a catalytically inactive SH-enzyme from the catalytically active acetyl-S-enzyme
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formation of a catalytically inactive SH-enzyme from the catalytically active acetyl-S-enzyme
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5 mM, complete inhibition
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no inhibition of wild type enzyme, inhibits activity of D56A and D56E mutant enzymes
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3D Structure of Enzyme-Ligand-Complex (PDB) (38 results)

Enzyme Kinetic Parameters

KM Value (7 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
11
-
pH 7.7, 37°C
7.5
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pH 7.7, 30°C

IC50 Value (2 results)

EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE

References & Links

Links to other databases for dithioerythritol

ChEBI
PubChem
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PubChem