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BRENDA support

Ligand pyridoxamine 5'-phosphate

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Basic Ligand Information

Molecular Structure
Picture of pyridoxamine 5'-phosphate (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C8H13N2O5P
pyridoxamine 5'-phosphate
ZMJGSOSNSPKHNH-UHFFFAOYSA-L
Synonyms:
pyridoxamine, 5'-phosphate, pyridoxamine-5'-phosphate, Pyridoxamine 5-phosphate, pyridoxamine phosphate
Pathway Source
Pathways
BRENDA
vitamin B6 metabolism
KEGG
Vitamin B6 metabolism, Vitamin digestion and absorption
MetaCyc
pyridoxal 5'-phosphate salvage I, pyridoxal 5'-phosphate salvage II (plants)


Show all pahtways known for Show all BRENDA pathways known for pyridoxamine 5'-phosphate

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (2 results)

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EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE

In Vivo Product in Enzyme-catalyzed Reactions (5 results)

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EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
ATP + pyridoxamine = ADP + pyridoxamine 5'-phosphate
show the reaction diagram
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L-alanine + pyridoxal 5'-phosphate = pyruvate + pyridoxamine 5'-phosphate
show the reaction diagram
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D-alanine + pyridoxal 5'-phosphate = pyridoxamine 5'-phosphate + pyruvate
show the reaction diagram
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Substrate in Enzyme-catalyzed Reactions (6 results)

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EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
pyridoxamine 5'-phosphate + pyruvate = pyridoxal 5'-phosphate + L-alanine
show the reaction diagram
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pyridoxamine 5'-phosphate + 2-oxoglutarate = pyridoxal 5'-phosphate + D-glutamate
show the reaction diagram
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pyridoxamine 5'-phosphate + H2O = pyridoxamine + phosphate
show the reaction diagram
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pyridoxamine 5'-phosphate + H2O = pyridoxamine + phosphate
show the reaction diagram
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Product in Enzyme-catalyzed Reactions (16 results)

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EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
ATP + pyridoxamine = ADP + pyridoxamine 5'-phosphate
show the reaction diagram
-
L-alanine + pyridoxal 5'-phosphate = pyruvate + pyridoxamine 5'-phosphate
show the reaction diagram
-
D-alanine + pyridoxal 5'-phosphate = pyridoxamine 5'-phosphate + pyruvate
show the reaction diagram
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2-mercaptoethanol + L-serine + pyridoxal phosphate = S-pyruvylmercaptoethanol + pyridoxamine phosphate + H2O
show the reaction diagram
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Enzyme Cofactor/Cosubstrate (185 results)

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EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
bound to enzyme E1 through an ionic interaction with a positive charge on the surface of the enzyme, the cofactor is needed for the binding of the substrate to the enzyme
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can replace pyridoxal 5'-phosphate, Km: 0.00715 mM
can replace pyridoxal 5'-phosphate
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reactivates after dissociation of the coenzyme
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Activator in Enzyme-catalyzed Reactions (10 results)

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EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
shows 20% of pyridoxal 5'-phosphate activation
in the presence of pyruvate
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Inhibitor in Enzyme-catalyzed Reactions (13 results)

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EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
slight
-
approx. 0.1 mM, 23% inhibition of isozyme A
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substrate inhibition above 0.0006 mM
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weak inhibition of phosphorylase I, not II
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0.25 mM, 60% inactivation after 20 min
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0.5 mM, 50% inhibition of pyridoxine 5’-phosphate hydrolysis, less effective than pyridoxal 5’-phosphate or 4-pyridoxic acid 5’-phosphate
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complete inhibition at 0.2 mM
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3D Structure of Enzyme-Ligand-Complex (PDB) (450 results)

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EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (8 results)

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EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.45
-
pH 7.4, 37°C, recombinant enzyme

KM Value (38 results)

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EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
3.4
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mutant E68A, pH 9.0, 30°C
0.004
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pH 8.0, 37°C
0.0032
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pH 8.0, 37°C

Ki Value (2 results)

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EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE

References & Links

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Links to other databases for pyridoxamine 5'-phosphate

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ChEBI
PubChem
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PubChem