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Ligand neomycin C

Basic Ligand Information

Molecular Structure

Show all BRENDA pathways known for neomycin C

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (5 results)

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Show Substrate (5 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.3.1.82

acetyl-CoA + neomycin C = CoA + 6'-N-acetylneomycin C

755691

-

2.3.1.82

acetyl-CoA + neomycin C = CoA + N6'-acetylneomycin

658002

-

2.3.1.82

acetyl-CoA + neomycin C = CoA + N6'-acetylneomycin C

636898, 636899, 636910, 636913

-

2.3.1.82

3 entries

2.6.1.95

neomycin C + 2-oxoglutarate = 6'''-hydroxyneomycin C + L-glutamate

719676

-

2.7.7.46

ATP + neomycin C = diphosphate + 2''-adenylylneomycin C

645289

-

Activator in Enzyme-catalyzed Reactions (1 result)

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Show Activating Compound (1 entry)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

3.6.5.3

-

644144

-

Inhibitor in Enzyme-catalyzed Reactions (1 result)

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Show Inhibitors (1 entry)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

2.7.7.46

dead-end inhibition, uncompetitive versus MgATP2-

645289

-

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (5 results)

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Show Turnover Number (5 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

2.3.1.82

0.057

-

pH 8.0, 37°C

755691

2.3.1.82

0.06

-

pH 8.0, 37°C

755691

2.3.1.82

0.135

-

pH 6.0, 37°C, mutant enzyme W164A

658002

2.3.1.82

0.205

-

pH 6.0, 37°C

636910

2.3.1.82

0.205

-

pH 6.0, 37°C, wild-type enzyme

658002

2.3.1.82

5 entries

K_M Value (5 results)

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Show KM Value (5 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.3.1.82

0.00531

-

pH 6.0, 37°C

636910

2.3.1.82

0.0074

-

S 0.5 value, Hill coefficient 1.43, pH 8.0, 37°C

755691

2.3.1.82

0.0079

-

S 0.5 value, Hill coefficient 2.05, pH 8.0, 37°C

755691

2.3.1.82

0.014

-

wild-type enzyme

636913

2.3.1.82

0.25

-

mutant enzyme C109A/C70A

636913

2.3.1.82

5 entries

References & Links

Literature References (9)

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Show Reference (9 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

636898

4935296

Enzymatic acetylation of aminoglycoside antibiotics by Escherichia coli carrying an R factor

1971

Benveniste, R.; Davies, J.

Biochemistry

10

1787-1796

636899

4614862

Resistance to aminosides induced by an isoenzyme, kanamycin acetyltransferase

1974

Le Goffic, F.; Martel, A.

Biochimie

56

893-897

636910

9145851

Overexpression and characterization of the chromosomal aminoglycoside 6'-N-acetyltransferase from Enterococcus faecium

1997

Wright, G.D.; Ladak, P.

Antimicrob. Agents Chemother.

41

956-960

636913

11297438

Kinetic and mutagenic characterization of the chromosomally encoded Salmonella enterica AAC(6')-Iy aminoglycoside N-acetyltransferase

2001

Magnet, S.; Lambert, T.; Courvalin, P.; Blanchard, J.S.

Biochemistry

40

3700-3709

644144

6114863

Effects of antibiotics, N-acetylaminoacyl-tRNA and other agents on the elongation-factor Tu dependent and ribosome-dependent GTP hydrolysis promoted by 2'(3')-O-L-phenylalanyladenosine

1981

Campuzano, S.; Modolell, J.

Eur. J. Biochem.

117

27-31

645289

2841976

Alternative substrate and inhibition kinetics of aminoglycoside nucleotidyltransferase 2''-I in support of a Theorell-Chance kinetic mechanism

1988

Gates, C.A.; Northrop, D.B.

Biochemistry

27

3826-3833

658002

12767240

Kinetic mechanism of the GCN5-related chromosomal aminoglycoside acetyltransferase AAC(6')-Ii from Enterococcus faecium: evidence of dimer subunit cooperativity

2003

Draker, K.A.; Northrop, D.B.; Wright, G.D.

Biochemistry

42

6565-6574

719676

21689223

The oxidoreductases LivQ and NeoQ are responsible for the different 6-modifications in the aminoglycosides lividomycin and neomycin

2011

Clausnitzer, D.; Piepersberg, W.; Wehmeier, U.F.

J. Appl. Microbiol.

111

642-651

755691

27785912

Structural and biochemical characterization of Acinetobacter spp. aminoglycoside acetyltransferases highlights functional and evolutionary variation among antibiotic resistance enzymes

2017

Stogios, P.; Kuhn, M.; Evdokimova, E.; Law, M.; Courvalin, P.; Savchenko, A.

ACS Infect. Dis.

3

132-143

Links to other databases for neomycin C

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Show Links (1 entry)

ChEBI

PubChem