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2-methylbutanoyl-CoA + acceptor = 2-methyl-2-butenoyl-CoA + reduced acceptor
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2-methylbutyryl-CoA + electron acceptor = 2-methyl-2-butenoyl-CoA + reduced acceptor
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2-methylbutanoyl-CoA + acceptor = 2-methylbut-2-enoyl-CoA + reduced acceptor
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2-methylbutanoyl-CoA + electron transfer flavoprotein = 2-methylbut-2-enoyl-CoA + reduced electron transfer flavoprotein
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2-methylbutanoyl-CoA + electron-transfer flavoprotein = (E)-2-methylbut-2-enoyl-CoA + reduced electron-transfer flavoprotein + H+
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2-methylbutyryl-CoA + malonyl-[acyl-carrier protein] = 3-oxo-4-methyl-hexanoyl-[acyl-carrier protein] + CoA + CO2
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2-methylbutyryl-CoA + malonyl-[acyl-carrier protein] = 3-oxo-4-methylhexanoyl-[acyl-carrier protein] + CoA + CO2
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2-methylbutanoyl-CoA + phosphate = 2-methylbutanoyl phosphate + CoA
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2-methylbutanoyl-CoA + phosphate = CoA + 2-methylbutanoyl phosphate
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2-methylbutanoyl-CoA + malonyl-[acyl-carrier protein] = 4-methyl-3-oxohexanoyl-[acyl-carrier-protein] + CoA + CO2
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3-methyl-2-oxopentanoate + CoA + NAD+ = 2-methylbutanoyl-CoA + CO2 + NADH
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3-methyl-2-oxopentanoate + CoA + NAD+ = 2-methylbutanoyl-CoA + CO2 + NADH + H+
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2-keto-3-methylvalerate + CoA + oxidized ferredoxin = 2-methylbutanoyl-CoA + CO2 + reduced ferredoxin
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2-methyl-trans-crotonyl-CoA + NADPH = 2-methylbutanoyl-CoA + NADP+
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ATP + 2-methylbutyrate + CoA = ADP + phosphate + 2-methylbutyryl-CoA
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ATP + 2-methylbutyrate + CoA = AMP + diphosphate + 2-methylbutyryl-CoA
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ATP + methylbutyrate + CoA = AMP + diphosphate + methylbutyryl-CoA
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ATP + 2-methylbutyrate + CoA = ADP + phosphate + 2-methylbutyryl-CoA
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