Ligand view of 1,N6-etheno-NAD+ (5453 - JCDBQDNBEQHDHK-BSLNIGMPSA-O)

C₂₃H₂₇N₇O₁₄P₂

1,N6-etheno-NAD+

JCDBQDNBEQHDHK-BSLNIGMPSA-O

1.1.1.27

(S)-lactate + epsilon-NAD+ = pyruvate + epsilon-NADH + H+

286471

-

1.1.1.282

L-quinate + nicotinamide 1,N6-ethenoadenine dinucleotide = 3-dehydroquinate + ?

654384

-

1.1.1.37

nicotinamide 1-N6-ethenoadenine dinucleotide + L-malate = ?

286671

2x0j, 3D-view

1.4.1.1

L-Ala + H2O + 1,N6-etheno-NAD+ = pyruvate + NH3 + 1,N6-etheno-NADH

349547, 349546

-

2.4.2.30

nicotinamide 1,N6-ethenoadenine dinucleotide + (ADP-D-ribosyl)n-vH-Ras protein = nicotinamide + ?

736518

-

2.4.2.37

etheno-NAD+ + [dinitrogen reductase] = ?

-

nicotinamide 1,N6-ethenoadenine dinucleotide + H2O = NMN + N6-ethenoadenosine 5'-phosphate

246814, 246819, 246809

-

1.1.1.282

69% of the activity with NAD+

-

0.02 mM, 95% residual activity in presence of 0.05 mM NAD, pH 7.4

-

-

-

-

-

-

-

1.464

-

286671

2.4.2.30

0.0225

-

at pH 7.5 and 22Â°C

0.054

-

pH and temperature not specified in the publication

715672

137060

-

Pyridine nucleotide-linked glycohydrolases

1989

Price, S.R.; Pekala, P.H.

Coenzymes and cofactors, Glutathione, Chem. Biochem. Med. Aspects Pt. A (Dolphin D, Poulson R, Avromonic O, eds. ) John Wiley & Sons, New York

2

514-548

137068

212424

Studies of bovine erythrocyte NAD glycohydrolase

1978

Pekala, P.H.; Anderson, B.M.

J. Biol. Chem.

253

7453-7459

137082

6260772

Purification and properties of the soluble NAD glycohydrolase from Bungaris fascilatus venom

1981

Yost, D.A.; Anderson, B.M.

J. Biol. Chem.

256

3647-3653

137088

9193688

Characteriation of hydrosoluble and detergent-solubilized forms of mitochondrial NAD+ glycohydrolase from bovine liver

1997

Jorcke, D.; Ziegler, M.; Schweiger, M.

Adv. Exp. Med. Biol.

419

447-451

209860

214117

Catabolism of NAD+ in rabbit reticulocyte lysates

1978

Wu, J.M.; Lennon, M.B.; Suhadolnik, R.J.

Biochim. Biophys. Acta

520

588-597

246809

9453136

Characterization of H. parasuis periplasmic nucleotide pyrophosphatase as a potential target enzyme for inhibition of growth

1997

Wise, D.J.; Anderson, C.D.; Anderson, B.M.

Vet. Microbiol.

58

261-276

246814

3009442

Characterization of Haemophilus influenzae nucleotide pyrophosphatase. An enzyme of critical importance for growth of the organism

1996

Kahn, D.W.; Anderson, B.M.

J. Biol. Chem.

261

6016-6025

246819

6109518

Studies of bovine seminal fluid nucleotide pyrophosphatase

1980

Buckon, M.E.; Anderson, B.M.

Arch. Biochem. Biophys.

202

396-404

286471

1989512

Kinetic mechanism of the endogenous lactate dehydrogenase activity of duck epsilon-crystallin

1991

Chang, G.G.; Huang, S.M.; Chiou, S.H.

Arch. Biochem. Biophys.

284

285-291

286671

9244395

Purification and kinetic characterization of Haemophilus parasuis malate dehydrogenase

1997

Wise, D.J.; Anderson, C.D.; Anderson, B.M.

Arch. Biochem. Biophys.

344

176-183

326409

10825668

NAD(P)+-glycohydrolase from human spleen: a multicatalytic enzyme

2000

Orsomando, G.; Polzonetti, V.; Natalini, P.

Comp. Biochem. Physiol. B

126

89-98

326410

-

Human CD38 is an authentic NAD(P)+ glycohydrolase

1998

Berthelier, V.; Tixier, J.M.; Muller-Steffner, H.; Schuber, F.; Deterre, P.

Biochem. J.

330

1383-1390

349546

2501471

A further characterization of alanine dehydrogenase from Streptomyces aureofaciens

1989

Vancurova, I.; Vancura, A.; Volc, J.; Neuzil, J.; Behal, V.

J. Basic Microbiol.

29

185-189

349547

2917562

Alanine dehydrogenase from Streptomyces fradiae. Purification and properties

1989

Vancura, A.; Vancurova, I.; Volc, J.; Jones, S.K.T.; Flieger, M.; Basarova, G.; Behal, V.

Eur. J. Biochem.

179

221-227

391690

3269392

Valine dehydrogenase from Streptomyces fradiae: purification and properties

1988

Vancura, A.; Vancurova, I.; Volc, J.; Fussey, S.P.M.; Flieger, M.; Neuzil, J.; Marsalek, J.; Behal, V.

J. Gen. Microbiol.

134

3213-3219

391695

3182727

Isolation and characterization of valine dehydrogenase from Streptomyces aureofaciens

1988

Vancurova, I.; Vancura, A.; Volc, J.; Neuzil, J.; Flieger, M.; Basarova, G.; Behal, V.

J. Bacteriol.

170

5192-5196

391702

7669808

Purification and characterization of a novel valine dehydrogenase from Streptomyces aureofaciens

1995

Nguyen, L.T.; Nguyen, K.T.; Kopecky, J.; Nova, P.; Novotna, J.; Behal, V.

Biochim. Biophys. Acta

1251

186-190

391703

-

The tylosin producer, Streptomyces fradiae, contains a second valine dehydrogenase

1995

Nguyen, L.T.; Nguyen, K.T.; Spizek, J.; Behal, V.

Microbiology

141

1139-1145

489801

3141411

Purification and properties of dinitrogenase reductase ADP-ribosyltransferase from the photosynthetic bacterium Rhodospirillum rubrum

1988

Lowery, R.G.; Ludden, P.W.

J. Biol. Chem.

263

16714-16719

654384

-

Hydroaromatic metabolism in Rhodococcus rhodochrous: purification and characterization of its NAD-dependent quinate dehydrogenase

1990

Bruce, N.C.; Cain, R.B.

Arch. Microbiol.

154

179-186

655836

11114931

Vibrio fischeri genes hvnA and hvnB encode secreted NAD(+)-glycohydrolases

2001

Stabb, E.V.; Reich, K.A.; Ruby, E.G.

J. Bacteriol.

183

309-317

656778

12083366

NAD-dependent inhibition of the NAD-glycohydrolase activity in A549 cells

2002

Balducci, E.; Micossi, L.G.

Mol. Cell. Biochem.

233

127-132

710157

20183597

Evaluation of NAD(H) analogues as selective inhibitors for Trypanosoma cruzi S-adenosylhomocysteine hydrolase

2009

Li, Q.; Cai, S.; Fang, J.; Borchardt, R.; Kuczera, K.; Middaugh, C.; Schowen, R.

Nucleosides Nucleotides Nucleic Acids

28

473-484

714434

21641214

Flavonoids as inhibitors of human CD38

2011

Kellenberger, E.; Kuhn, I.; Schuber, F.; Muller-Steffner, H.

Bioorg. Med. Chem. Lett.

21

3939-3942

714452

20951593

Identification by high-throughput screening of inhibitors of Schistosoma mansoni NAD(+) catabolizing enzyme

2010

Kuhn, I.; Kellenberger, E.; Said-Hassane, F.; Villa, P.; Rognan, D.; Lobstein, A.; Haiech, J.; Hibert, M.; Schuber, F.; Muller-Steffner, H.

Bioorg. Med. Chem.

18

7900-7910

715672

21398623

A high-throughput fluorescence resonance energy transfer-based assay for DNA ligase

2011

Shapiro, A.B.; Eakin, A.E.; Walkup, G.K.; Rivin, O.

J. Biomol. Screen.

16

486-493

731357

24721563

Probing the catalytic mechanism of bovine CD38/NAD+ glycohydrolase by site directed mutagenesis of key active site residues

2014

Kuhn, I.; Kellenberger, E.; Cakir-Kiefer, C.; Muller-Steffner, H.; Schuber, F.

Biochim. Biophys. Acta

1844

1317-1331

736518

26850638

Identification of inhibitors of Pseudomonas aeruginosa exotoxin-S ADP-ribosyltransferase activity

2016

Pinto, A.F.; Ebrahimi, M.; Saleeb, M.; Forsberg, A.; Elofsson, M.; Schueler, H.

J. Biomol. Screen.

21

590-595

-

Ligand 1,N6-etheno-NAD+

Basic Ligand Information

Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (14 results)

Enzyme Cofactor/Cosubstrate (10 results)

Inhibitor in Enzyme-catalyzed Reactions (4 results)

3D Structure of Enzyme-Ligand-Complex (PDB) (1 result)

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (3 results)

K_M Value (12 results)

IC₅₀ Value (1 result)

References & Links

Literature References (28)

Links to other databases for 1,N6-etheno-NAD+

Ligand 1,N6-etheno-NAD+

Basic Ligand Information

Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (14 results)

Enzyme Cofactor/Cosubstrate (10 results)

Inhibitor in Enzyme-catalyzed Reactions (4 results)

3D Structure of Enzyme-Ligand-Complex (PDB) (1 result)

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (3 results)

KM Value (12 results)

IC50 Value (1 result)

References & Links

Literature References (28)

Links to other databases for 1,N6-etheno-NAD+

k_cat Value (Turnover Number) (3 results)

K_M Value (12 results)

IC₅₀ Value (1 result)