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Ligand neokestose

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

In Vivo Product in Enzyme-catalyzed Reactions (1 result)

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Show Natural Product (1 entry)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.243

1-kestose + 3 sucrose = 3 neokestose + H2O

0

-

Substrate in Enzyme-catalyzed Reactions (19 results)

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Show Substrate (19 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.10

6G-kestotriose + sucrose = 6-kestotriose + ?

736970

-

2.4.1.100

6G-kestose + 6G-kestose = ?

488350

-

2.4.1.100

6G-kestose + sucrose = tetrasaccharide

488350

-

2.4.1.100

1-kestotriose + 6G-kestotriose = 1,1-kestotetraose + ?

736970

-

2.4.1.243

neokestose + sucrose = 1-kestose + ?

664516

-

2.4.1.243

neokestose + sucrose = 1F,6G-di-beta-D-fructofuranosylsucrose + ?

660021

-

2.4.1.99

neo-kestose = 1G-kestotetraose + 6G-kestotetraose

689242

-

2.4.1.99

sucrose + neokestose = D-glucose + 1F,6G-di-beta-fructofuranosylsucrose

489628

-

3.2.1.153

6G-kestotriose + H2O = ?

208824

-

3.2.1.153

neokestose + H2O = ?

657048

-

3.2.1.154

6G-kestotriose + H2O = ?

732665

-

3.2.1.154

6G-kestotriose + H2O = sucrose + fructose

208823

-

3.2.1.154

neokestose + H2O = ?

657006

-

2.4.1.100

3 entries

2.4.1.243

2 entries

2.4.1.99

2 entries

3.2.1.153

2 entries

3.2.1.154

3 entries

3.2.1.26

neokestose + H2O = ?

708103

-

3.2.1.80

6G-kestotriose + H2O = ?

732665, 681133

-

3.2.1.80

neokestose + H2O = ?

754296, 714715

-

3.2.1.80

neokestose + H2O = beta-D-fructose + ?

670976

-

3.2.1.80

neokestose + H2O = beta-D-fructose + sucrose

657048

-

3.2.1.80

neokestose + H2O = D-fructose + sucrose

208823

-

3.2.1.80

5 entries

Product in Enzyme-catalyzed Reactions (9 results)

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Show Product (9 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.10

sucrose + sucrose = 1-kestose + 6-kestose + neo-kestose + ?

0

-

2.4.1.10

sucrose + sucrose = D-glucose + neokestose

0

-

2.4.1.10

2 entries

2.4.1.100

sucrose + 1-kestose = 1-kestose + 6G-kestose + tetrasaccharide

488350

-

2.4.1.243

1-kestose + 3 sucrose = 3 neokestose + H2O

0

-

2.4.1.243

1-kestose + sucrose = D-sucrose + neokestose

0

-

2.4.1.243

sucrose = neokestose

0

-

3.2.1.154

6G,1,6-kestopentaose + H2O = fructose + 6G-kestotriose + ?

0

-

3.2.1.154

6G,6-kestotetraoase + H2O = fructose + 6G-kestotriose

0

-

2.4.1.243

3 entries

3.2.1.154

2 entries

3.2.1.80

6G,6-kestotetraose + H2O = D-fructose + neokestose

208823

-

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (1 result)

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Show Turnover Number (1 entry)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

3.2.1.26

142.7

-

pH 5.7, 37°C

708103

K_M Value (1 result)

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Show KM Value (1 entry)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

3.2.1.26

1.7

-

pH 5.7, 37°C

708103

References & Links

Literature References (16)

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Show Reference (16 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

208823

8742337

Purification and characterization of an oat fructan exohydrolase that preferentially hydrolyzes beta-2,6-fructans

1996

Henson, C.A.; Livingston III, D.P.

Plant Physiol.

110

639-644

208824

-

Characterization of a fructan exohydrolase purified from barley stems that hydrolyzes multiple fructofuranosidic linkages

1998

Henson, C.A.; Livingston III, D.P.

Plant Physiol. Biochem.

36

715-720

488350

100201941

A fructan: fructan fructosyltransferase activity from Lolium rigidum

1997

St.John, J.A.; Bonnett, G.D.; Simpson, R.J.; Tanner, G.J.

New Phytol.

135

235-247

489628

-

Purification and characterization of sucrose:sucrose 1-fructosyltransferase from the roots of Asparagus (Asparagus officinalis L.)

1980

Shiomi, N.; Izawa, M.

Agric. Biol. Chem.

44

603-614

657006

14617070

Unexpected presence of fructan 6-exohydrolases (6-FEHs) in non-fructan plants: characterization, cloning, mass mapping and functional analysis of a novel 'cell-wall invertase-like' specific 6-FEH from sugar beet (Beta vulgaris L.)

2003

Van den Ende, W.; De Coninck, B.; Clerens, S.; Vergauwen, R.; Van Laere, A.

Plant J.

36

697-710

657048

12586886

Fructan 1-exohydrolases. beta-(2,1)-trimmers during graminan biosynthesis in stems of wheat? Purification, characterization, mass mapping, and cloning of two fructan 1-exohydrolase isoforms

2003

Van Den Ende, W.; Clerens, S.; Vergauwen, R.; Van Riet, L.; Van Laere, A.; Yoshida, M.; Kawakami, A.

Plant Physiol.

131

621-631

660021

15720662

Purification and characterization of a fructosyltransferase from onion bulbs and its key role in the synthesis of fructo-oligosaccharides in vivo

2005

Fujishima, M.; Sakai, H.; Ueno, K.; Takahashi, N.; Onodera, S.; Benkeblia, N.; Shiomi, N.

New Phytol.

165

513-524

664516

-

Reverse reactions of fructosyl transfer catalyzed by asparagus 6G-fructosyltransferase

1982

Shiomi, N.

Carbohydr. Res.

106

166-169

670976

15501176

Plant fructan exohydrolases: a role in signaling and defense?

2004

Van den Ende, W.; De Coninck, B.; Van Laere, A.

Trends Plant Sci.

9

523-528

681133

17456505

Cloning, gene mapping, and functional analysis of a fructan 1-exohydrolase (1-FEH) from Lolium perenne implicated in fructan synthesis rather than in fructan mobilization

2007

Lothier, J.; Lasseur, B.; Le Roy, K.; Van Laere, A.; Prudhomme, M.P.; Barre, P.; Van den Ende, W.; Morvan-Bertrand, A.

J. Exp. Bot.

58

1969-1983

689242

18346102

Coordinated expression of functionally diverse fructosyltransferase genes is associated with fructan accumulation in response to low temperature in perennial ryegrass

2008

Hisano, H.; Kanazawa, A.; Yoshida, M.; Humphreys, M.O.; Iizuka, M.; Kitamura, K.; Yamada, T.

New Phytol.

178

766-780

708103

20380746

Characterization of recombinant beta-fructofuranosidase from Bifidobacterium adolescentis G1

2010

Omori, T.; Ueno, K.; Muramatsu, K.; Kikuchi, M.; Onodera, S.; Shiomi, N.

Chem. Cent. J.

4

09

714715

21463533

Cloning and functional characterization of a fructan 1-exohydrolase (1-FEH) in edible burdock (Arctium lappa L.)

2011

Ueno, K.; Ishiguro, Y.; Yoshida, M.; Onodera, S.; Shiomi, N.

Chem. Cent. J.

5

16

732665

25023629

Cloning and characterization of a novel fructan 6-exohydrolase strongly inhibited by sucrose in Lolium perenne

2014

Lothier, J.; Van Laere, A.; Prudhomme, M.P.; Van den Ende, W.; Morvan-Bertrand, A.

Planta

240

629-643

736970

24104051

Fructan metabolism in developing wheat (Triticum aestivum L.) kernels

2013

Verspreet, J.; Cimini, S.; Vergauwen, R.; Dornez, E.; Locato, V.; Le Roy, K.; De Gara, L.; Van den Ende, W.; Delcour, J.A.; Courtin, C.M.

Plant Cell Physiol.

54

2047-2057

754296

29931209

Purification, characterization, and functional analysis of a novel 6G&1-FEH mainly hydrolyzing neokestose from Asparagus

2018

Ueno, K.; Sonoda, T.; Yoshida, M.; Shiomi, N.; Onodera, S.

J. Exp. Bot.

69

4295-4308

Links to other databases for neokestose

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ChEBI

PubChem

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