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Ligand Mannopentaose

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (22 results)

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Show Substrate (22 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.99.18

mannopentaose + 1,4-benzoquinone = ? + reduced 1,4-benzoquinone

724019

-

2.4.1.319

beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate = beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate

725502

-

2.4.1.319

beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate = beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate

725502

-

2.4.1.319

[(1->4)-beta-D-mannosyl]5 + alpha-D-mannose 1-phosphate = [(1->4)-beta-D-mannosyl]6 + phosphate

725502

-

2.4.1.319

[(1->4)-beta-D-mannosyl]5 + phosphate = [(1->4)-beta-D-mannosyl]4 + alpha-D-mannose 1-phosphate

725502

-

2.4.1.319

4 entries

3.1.1.72

(mannose)5 + triacetin = ?

650392

-

3.2.1.100

mannopentaose + H2O = D-mannose + ?

751110

-

3.2.1.100

mannopentaose + H2O = mannobiose + mannose

135706

-

3.2.1.100

2 entries

3.2.1.25

mannopentaose + H2O = ?

136031

-

3.2.1.78

Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O = Man-beta-1,4-Man-beta-1,4-Man + Man-beta-1,4-Man

714350

-

3.2.1.78

Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O = Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Man

678041

-

3.2.1.78

Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O = Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + mannose

666074, 666855

-

3.2.1.78

Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O = Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + mannose

741723

-

3.2.1.78

mannopentaose + H2O = ?

746948, 731856, 749657

-

3.2.1.78

mannopentaose + H2O = mannobiose + mannotriose

749688

-

3.2.1.78

mannopentaose + H2O = mannobiose + mannotriose + mannotetraose

750179

-

3.2.1.78

mannopentaose + H2O = mannose + mannobiose + mannotriose + mannotetraose

208785, 208798, 208814, 208786, 208796, 208790, 208800, 208799, 208787, 208784

-

3.2.1.78

mannopentaose + H2O = mannose + mannobiose + mannotriose + mannotetraose + mannopentaose

750157

-

3.2.1.78

mannopentaose + H2O = mannose + mannotetraose

732893

-

3.2.1.78

mannopentaose + H2O = mannotetraose + ?

751779

-

3.2.1.78

mannopentaose + H2O = mannotriose + mannobiose

657124

-

3.2.1.78

mannopentaose + H2O = mannotriose + mannobiose + mannotetraose

750436

-

3.2.1.78

13 entries

Product in Enzyme-catalyzed Reactions (22 results)

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Show Product (22 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.319

beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate = beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate

-

-

2.4.1.319

[(1->4)-beta-D-mannosyl]4 + alpha-D-mannose 1-phosphate = [(1->4)-beta-D-mannosyl]5 + phosphate

-

-

2.4.1.319

[(1->4)-beta-D-mannosyl]6 + phosphate = [(1->4)-beta-D-mannosyl]5 + alpha-D-mannose 1-phosphate

-

-

2.4.1.319

3 entries

3.2.1.100

mannotriose + mannose = mannopentaose

135706

-

3.2.1.78

Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O = Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + mannose

-

-

3.2.1.78

Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O = Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Man + mannose + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man

-

-

3.2.1.78

galactomannan + H2O = mannose + mannobiose + mannotriose + mannotetraose + galactose-linked mannotetraose + mannopentaose + mannohexaose

-

-

3.2.1.78

guar gum + H2O = mannose + mannobiose + mannotriose + mannotetraose + galactose-linked mannotetraose + mannopentaose + mannohexaose

-

-

3.2.1.78

insoluble mannan + H2O = mannotriose + mannotetraose + mannopentaose

-

-

3.2.1.78

ivory nut mannan + H2O = mannose + mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose

-

-

3.2.1.78

ivory nut mannan + H2O = mannotriose + mannotetraose + mannopentaose + mannohexaose

-

-

3.2.1.78

konjac glucomannan + H2O = mannose + mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose

-

-

3.2.1.78

konjac powder + H2O = mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose + mannoheptaose

-

-

3.2.1.78

locust bean galactomannan + H2O = mannotriose + mannotetraose + mannopentaose

-

-

3.2.1.78

locust bean gum + H2O = mannopentaose

-

-

3.2.1.78

locust bean gum + H2O = mannose + mannobiose + mannotriose + mannotetraose + galactose-linked mannotetraose + mannopentaose + mannohexaose

-

-

3.2.1.78

locust bean gum + H2O = mannose + mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose

-

-

3.2.1.78

locust bean gum + H2O = mannose + mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose + mannoheptaose

-

-

3.2.1.78

locust bean gum galactomannan + H2O = mannose + mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose

-

-

3.2.1.78

mannohexaose + H2O = mannotriose + mannotetraose + mannopentaose + mannobiose

-

-

3.2.1.78

mannopentaose + H2O = mannose + mannobiose + mannotriose + mannotetraose + mannopentaose

-

-

3.2.1.78

mannotetraose + H2O = mannobiose + mannotriose + mannopentaose

-

-

3.2.1.78

18 entries

Inhibitor in Enzyme-catalyzed Reactions (1 result)

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Show Inhibitors (1 entry)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

5.3.1.1

-

705563

-

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (17 results)

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Show Turnover Number (17 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

1.1.99.18

7.4

-

isoform CDH IIB, at pH 6.0 and 30°C

724019

1.1.99.18

19.8

-

isoform CDH IIA, at pH 6.0 and 30°C

724019

2.4.1.319

20.5

-

pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate

725502

2.4.1.319

20.5

-

pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction

725502

2.4.1.319

31.9

-

pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate

725502

2.4.1.319

31.9

-

pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction

725502

3.2.1.78

0.26

-

-

664068

3.2.1.78

3.9

-

30°C, pH 4.8

678041

3.2.1.78

11

-

pH 40°C, pH 4.5, mutant enzyme R171K

746948

3.2.1.78

13

-

pH 40°C, pH 4.5, wild-type enzyme

746948

3.2.1.78

45

-

-

666074

3.2.1.78

52.42

-

-

664068

3.2.1.78

97

-

at pH 6.0 and 37°C

750179

3.2.1.78

117

-

mutant W283S, pH 5.5, 37°C

714350

3.2.1.78

134

-

wild-type, pH 5.5, 37°C

714350

3.2.1.78

148

-

mutant W283S, pH 5.5, 37°C

714350

3.2.1.78

193

-

pH 5.5, 37°C

714350

1.1.99.18

2 entries

2.4.1.319

4 entries

3.2.1.78

11 entries

K_M Value (15 results)

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Show KM Value (15 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.1.99.18

8.4

-

isoform CDH IIB, at pH 6.0 and 30°C

724019

1.1.99.18

39.2

-

isoform CDH IIA, at pH 6.0 and 30°C

724019

2.4.1.319

4.55

-

pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate

725502

2.4.1.319

4.55

-

pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction

725502

2.4.1.319

5.29

-

pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate

725502

2.4.1.319

5.29

-

pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction

725502

1.1.99.18

2 entries

2.4.1.319

4 entries

3.2.1.100

0.13

-

-

135706

3.2.1.78

0.08

-

pH 40°C, pH 4.5, wild-type enzyme

746948

3.2.1.78

0.6

-

wild-type, pH 5.5, 37°C

714350

3.2.1.78

1

-

mutant W283S, pH 5.5, 37°C

714350

3.2.1.78

1.3

-

mutant W283S, pH 5.5, 37°C

714350

3.2.1.78

1.61

-

-

666074

3.2.1.78

1.7

-

pH 40°C, pH 4.5, mutant enzyme R171K

746948

3.2.1.78

1.8

-

pH 5.5, 37°C

714350

3.2.1.78

5.3

-

30°C, pH 4.8

678041

3.2.1.78

8 entries

References & Links

Literature References (33)

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Show Reference (33 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

135706

7096283

Purification and characterization of a novel exo-beta-mannanase from Aeromonas sp. F-25

1982

Araki, T.; Kitamikado, M.

J. Biochem.

91

1181-1186

136031

-

Characterization of beta-mannosidase of an alkalophilic Bacillus sp.

1988

Akino, T.; Nakamura, N.; Horikoshi, K.

Agric. Biol. Chem.

52

1459-1464

208784

-

Enzyme to break down lettuce endosperm cell wall during gibberellin- and light-induced germination

1975

Halmer, P.; Bewley, J.D.; Thorpe, T.A.

Nature

258

716-718

208785

-

Isolierung und Eigenschaften einer beta-1,4-Mannanase aus Aspergillus niger

1976

Yamazaki, N.; Sinner, M.; Dietrichs, H.H.

Holzforschung

30

101-109

208786

-

Prification of a beta-mannanase enzyme from lucerne seed by substrate affinity chromatography

1978

McCleary, B.V.

Phytochemistry

17

651-653

208787

24407322

Mannanase production by the lettuce endosperm

1979

Halmer, P.; Bewley, J.D.

Planta

144

333-340

208790

-

Purification and characterization of an endo-beta-mannanase from Aeromonas sp. F-25

1983

Araki, T.

J. Fac. Agric. Kyushu Univ.

27

89-98

208796

-

Characterization of three beta-mannanases of an alkalophilic Bacillus sp.

1988

Akino, T.; Nakamura, N.; Horikoshi, K.

Agric. Biol. Chem.

52

773-779

208798

-

beta-D-Mannanase

1988

McCleary, B.V.

Methods Enzymol.

160

596-610

208799

-

Mannanse from Lactuca sativa: Metabolic requirements for production and partial purification

1989

Dulson, J.; Bewley, J.D.

Phytochemistry

28

363-369

208800

-

De novo synthesis of mannanase by the endosperm of Lactuca sativa

1989

Halmer, P.

Phytochemistry

28

371-379

208814

8534110

Purification and characterization of an extracellular mannanase from a marine bacterium, Vibrio sp. strain MA-138

1995

Tamaru, Y.; Araki, T.; Amagoi, H.; Mori, H.; Morishita, T.

Appl. Environ. Microbiol.

61

4454-4458

650392

14572903

Transacetylations to carbohydrates catalyzed by acetylxylan esterase in the presence of organic solvent

2003

Biely, P.; Wong, K.K.; Suckling, I.D.; Spanikova, S.

Biochim. Biophys. Acta

1623

62-71

657124

11469596

Molecular and biochemical characterization of endo-beta-mannanases from germinating coffee (Coffea arabica) grains

2001

Marraccini, P.; Rogers, W.J.; Allard, C.; Andre, M.L.; Caillet, V.; Lacoste, N.; Lausanne, F.; Michaux, S.

Planta

213

296-308

664068

16171384

The structure and characterization of a modular endo-beta-1,4-mannanase from Cellulomonas fimi

2005

Le Nours, J.; Anderson, L.; Stoll, D.; Stalbrand, H.; Lo Leggio, L.

Biochemistry

44

12700-12708

666074

16487541

Three-dimensional crystal structure and enzymic characterization of beta-mannanase Man5A from blue mussel Mytilus edulis

2006

Larsson, A.M.; Anderson, L.; Xu, B.; Munoz, I.G.; Uson, I.; Janson, J.C.; Stalbrand, H.; Stahlberg, J.

J. Mol. Biol.

357

1500-1510

666855

15840830

Three-dimensional structure of (1,4)-beta-D-mannan mannanohydrolase from tomato fruit

2005

Bourgault, R.; Oakley, A.J.; Bewley, J.D.; Wilce, M.C.

Protein Sci.

14

1233-1241

678041

16771710

Hydrolysis of (1,4)-beta-D-mannans in barley (Hordeum vulgare L.) is mediated by the concerted action of (1,4)-beta-D-mannan endohydrolase and beta-D-mannosidase

2006

Hrmova, M.; Burton, R.A.; Biely, P.; Lahnstein, J.; Fincher, G.B.

Biochem. J.

399

77-90

705563

19423633

Interaction of triosephosphate isomerase from the cell surface of Staphylococcus aureus and alpha-(1->3)-mannooligosaccharides derived from glucuronoxylomannan of Cryptococcus neoformans

2009

Furuya, H.; Ikeda, R.

Microbiology

155

2707-2713

714350

21867780

Recombinant production and characterisation of two related GH5 endo-beta-1,4-mannanases from Aspergillus nidulans FGSC A4 showing distinctly different transglycosylation capacity

2011

Dilokpimol, A.; Nakai, H.; Gotfredsen, C.H.; Baumann, M.J.; Nakai, N.; Hachem, M.A.; Svensson, B.

Biochim. Biophys. Acta

1814

1720-1729

724019

22729546

Characterization of the two Neurospora crassa cellobiose dehydrogenases and their connection to oxidative cellulose degradation

2012

Sygmund, C.; Kracher, D.; Scheiblbrandner, S.; Zahma, K.; Felice, A.K.; Harreither, W.; Kittl, R.; Ludwig, R.

Appl. Environ. Microbiol.

78

6161-6171

725502

23093406

Metabolic mechanism of mannan in a ruminal bacterium, Ruminococcus albus, involving two mannoside phosphorylases and cellobiose 2-epimerase: discovery of a new carbohydrate phosphorylase, beta-1,4-mannooligosaccharide phosphorylase

2012

Kawahara, R.; Saburi, W.; Odaka, R.; Taguchi, H.; Ito, S.; Mori, H.; Matsui, H.

J. Biol. Chem.

287

42389-42399

731856

25466024

A thermophilic beta-mannanase from Neosartorya fischeri P1 with broad pH stability and significant hydrolysis ability of various mannan polymers

2015

Yang, H.; Shi, P.; Lu, H.; Wang, H.; Luo, H.; Huang, H.; Yang, P.; Yao, B.

Food Chem.

173

283-289

732893

25082572

Structure-based investigation into the functional roles of the extended loop and substrate-recognition sites in an endo-beta-1,4-D-mannanase from the Antarctic springtail, Cryptopygus antarcticus

2014

Kim, M.K.; An, Y.J.; Song, J.M.; Jeong, C.S.; Kang, M.H.; Kwon, K.K.; Lee, Y.H.; Cha, S.S.

Proteins

82

3217-3223

741723

27193267

A thermostable GH26 endo-beta-mannanase from Myceliophthora thermophila capable of enhancing lignocellulose degradation

2016

Katsimpouras, C.; Dimarogona, M.; Petropoulos, P.; Christakopoulos, P.; Topakas, E.

Appl. Microbiol. Biotechnol.

100

8385-8397

746948

-

The role of subsite 2 of the Trichoderma reesei beta-mannanase TrMan5A in hydrolysis and transglycosylation

2012

Rosengren, A.; Hägglund, P.; Anderson, L.; Pavon-Orozco, P.; Peterson-Wulff, R.; Nerinckx, W.; Stalbrand, H.

Biocatal. Biotransform.

30

338-352

749657

26921423

A novel glycoside hydrolase family 113 endo-beta-1,4-mannanase from Alicyclobacillus sp. strain A4 and insight into the substrate recognition and catalytic mechanism of this family

2016

Xia, W.; Lu, H.; Xia, M.; Cui, Y.; Bai, Y.; Qian, L.; Shi, P.; Luo, H.; Yao, B.

Appl. Environ. Microbiol.

82

2718-2727

749688

28105485

Biochemical characterization of thermostable beta-1,4-mannanase belonging to the glycoside hydrolase family 134 from Aspergillus oryzae

2017

Sakai, K.; Mochizuki, M.; Yamada, M.; Shinzawa, Y.; Minezawa, M.; Kimoto, S.; Murata, S.; Kaneko, Y.; Ishihara, S.; Jindou, S.; Kobayashi, T.; Kato, M.; Shimizu, M.

Appl. Microbiol. Biotechnol.

101

3237-3245

750157

27714555

Biocatalytic characterization of an endo-beta-1,4-mannanase produced by Paenibacillus sp. strain HY-8

2017

Kim, D.Y.; Chung, C.W.; Cho, H.Y.; Rhee, Y.H.; Shin, D.H.; Son, K.H.; Park, H.Y.

Biotechnol. Lett.

39

149-155

750179

26558435

The GH5 1,4-beta-mannanase from Bifidobacterium animalis subsp. lactis Bl-04 possesses a low-affinity mannan-binding module and highlights the diversity of mannanolytic enzymes

2015

Morrill, J.; Kulcinskaja, E.; Sulewska, A.M.; Lahtinen, S.; Stalbrand, H.; Svensson, B.; Abou Hachem, M.

BMC Biochem.

16

26

750436

30037547

Gene cloning, expression, and X-ray crystallographic analysis of a beta-mannanase from Eisenia fetida

2018

Ueda, M.; Hirano, Y.; Fukuhara, H.; Naka, Y.; Nakazawa, M.; Sakamoto, T.; Ogata, Y.; Tamada, T.

Enzyme Microb. Technol.

117

15-22

751110

29997257

Structural basis of exo-beta-mannanase activity in the GH2 family

2018

Domingues, M.N.; Souza, F.H.M.; Vieira, P.S.; de Morais, M.A.B.; Zanphorlin, L.M.; Dos Santos, C.R.; Pirolla, R.A.S.; Honorato, R.V.; de Oliveira, P.S.L.; Gozzo, F.C.; Murakami, M.T.

J. Biol. Chem.

293

13636-13649

751779

26567779

NMR analysis of the binding mode of two fungal endo-beta-1,4-mannanases from GH5 and GH26 families

2016

Marchetti, R.; Berrin, J.G.; Couturier, M.; Ul Qader, S.A.; Molinaro, A.; Silipo, A.

Org. Biomol. Chem.

14

314-322

Links to other databases for Mannopentaose

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Show Links (1 entry)

ChEBI

PubChem

-