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Ligand aquacob(III)alamin

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (6 results)

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Show Substrate (6 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.6.2.2

aquacobalamin + NADH = reduced aquacobalamin + NAD+

394241

-

2.1.1.258

hydroxocobalamin + 5-methyltetrahydrofolate = methylcobalamin + tetrahydrofolate

718823

-

2.1.1.258

hydroxycobalamin + 5-methyltetrahydrofolate = methylcobalamin + tetrahydrofolate

720970

-

2.5.1.17

ATP + hydroxocobalamin = ?

652541

-

2.5.1.17

ATP + hydroxycobalamin = adenosylcobalamin + phosphate + diphosphate

690933

-

2.5.1.17

ATP + hydroxycobalamin = tripolyphosphate + adenosylcobalamin + H2O

690964

-

2.1.1.258

2 entries

2.5.1.17

3 entries

Product in Enzyme-catalyzed Reactions (1 result)

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Show Product (1 entry)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.1.1.258

methylcobalamin + tetrahydrofolate = hydroxocobalamin + 5-methyltetrahydrofolate

0

-

Enzyme Cofactor/Cosubstrate (2 results)

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Show Cofactor (2 entries)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

2.1.1.106

-

757761

-

5.4.99.2

required for Sbm activity

702794

-

Activator in Enzyme-catalyzed Reactions (4 results)

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Show Activating Compound (4 entries)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

1.17.4.2

-

437887

-

1.8.98.1

stimulates the activity of the purified H2: heterodisulfide oxidoreductase complex

655398

-

2.1.1.13

the addition of 0.05 mM aquacob(III)alamin to the crude extract results in an about 13fold increase in activity which is not greatly affected by the addition of cytochrome P450 reductase or reductase domain of neuronal nitric oxide synthase

703638

-

5.4.99.2

supplementation of hydroxocobalamin results in a marked increase in the holo-MCM activity in a dose-dependent manner, although the holo-MCM activity does not exceed 30% of the total-MCM activity even if hydroxocobalamin is supplemented at 10 mM

691535

-

Inhibitor in Enzyme-catalyzed Reactions (5 results)

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Show Inhibitors (5 entries)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

4.2.1.30

-

33818

-

4.3.1.7

-

34376

-

4.3.1.7

irreversible

34370

-

4.3.1.7

irreversible; noncompetitive

34382

-

4.3.1.7

3 entries

7.6.2.8

inhibits uptake to a similar extent as cyano-cobalamin

750412

-

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (4 results)

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Show Turnover Number (4 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

2.1.1.258

1.1

-

in 1 mM Tris-HCl (pH 7.6), at 25°C

718823

2.1.1.258

77.6

-

recombinant enzyme, in 0.1 M MES, at pH 5.1 and 25°C

720970

2.5.1.17

0.18

-

-

652541

2.5.1.17

0.18

-

+/- 0.01

652541

2.1.1.258

2 entries

2.5.1.17

2 entries

K_M Value (5 results)

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Show KM Value (5 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.6.2.2

0.0419

-

-

394241

2.1.1.258

0.058

-

recombinant enzyme, in 0.1 M MES, at pH 5.1 and 25°C

720970

2.1.1.258

2

-

in 1 mM Tris-HCl (pH 7.6), at 25°C

718823

2.5.1.17

0.003

-

-

652541

2.5.1.17

0.003

-

+/- 0.0004

652541

2.1.1.258

2 entries

2.5.1.17

2 entries

References & Links

Literature References (17)

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Show Reference (17 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

33818

14039344

A cobamide-requiring glycerol dehydrase from an acrolein-forming Lactobacillus

1962

Smiley, K.L.; Sobolov, M.

Arch. Biochem. Biophys.

97

538-543

34370

-

Ethanolamine deaminase (Clostridium sp.)

1971

Kaplan, B.H.; Stadtman, E.R.

Methods Enzymol.

17B

818-824

34376

5846988

The clostridial fermentations of choline and ethanolamine. II. Requirement for a cobamide coenzyme by an ethanolamine deaminase

1965

Bradbeer, C.

J. Biol. Chem.

240

4675-4681

34382

33657

Microbial metabolism of amino alcohols. Purification and properties of coenzyme B12-dependent ethanolamine ammonia-lyase of Escherichia coli

1978

Blackwell, C.M.; Turner, J.M.

Biochem. J.

175

555-563

394241

8027853

Cytochrome b5-like hemoprotein/cytochrome b5 reductase complex in rat liver mitochondria has NADH-linked aquacobalamin reductase activity

1994

Saido, H.; Watanabe, F.; Tamura, Y.; Miyatake, K.; Ito, A.; Yubisui, T.; Nakano, Y.

J. Nutr.

124

1037-1040

437887

14299643

Cobamides and ribonucleotide reduction. I. Cobamide stimulation of ribonucleotide reduction in extracts of Lactobacillus leichmannii

1965

Bakley, R.L.

J. Biol. Chem.

240

2173-2180

652541

15044458

The structural basis for methylmalonic aciduria. The crystal structure of archaeal ATP:cobalamin adenosyltransferase

2004

Saridakis, V.; Yakunin, A.F.; Xu, X.; Anandakumar, P.; Pennycooke, M.; Gu, J.; Cheung, F.; Lew, J.M.; Sanishvili, N.; Joachimiak, A.; Arrowsmith, C.H.; Edwards, A.M.; Christendat, D.

J. Biol. Chem.

279

23646-23653

655398

8119281

H2:heterodisulfide oxidoreductase complex from Methanobacterium thermoautotrophicum. Composition and properties

1994

Setzke, E.; Hedderich, R.; Heiden, S.; Thauer, R.K.

Eur. J. Biochem.

220

139-148

690933

18251506

Functional characterization and mutation analysis of human ATP:Cob(I)alamin adenosyltransferase

2008

Fan, C.; Bobik, T.A.

Biochemistry

47

2806-2813

690964

18452306

Structural characterization of a human-type corrinoid adenosyltransferase confirms that coenzyme B12 is synthesized through a four-coordinate intermediate

2008

St Maurice, M.; Mera, P.; Park, K.; Brunold, T.C.; Escalante-Semerena, J.C.; Rayment, I.

Biochemistry

47

5755-5766

691535

18710602

Cobalamin deficiency results in an abnormal increase in l-methylmalonyl-co-enzyme-A mutase expression in rat liver and COS-7 cells

2009

Nakao, M.; Hironaka, S.; Harada, N.; Adachi, T.; Bito, T.; Yabuta, Y.; Watanabe, F.; Miura, T.; Yamaji, R.; Inui, H.; Nakano, Y.

Br. J. Nutr.

101

492-498

702794

19806677

Investigating the role of native propionyl-CoA and methylmalonyl-CoA metabolism on heterologous polyketide production in Escherichia coli

2010

Zhang, H.; Boghigian, B.A.; Pfeifer, B.A.

Biotechnol. Bioeng.

105

567-573

703638

19243433

Cobalamin uptake and reactivation occurs through specific protein interactions in the methionine synthase-methionine synthase reductase complex

2009

Wolthers, K.R.; Scrutton, N.S.

FEBS J.

276

1942-1951

718823

7578120

Mechanistic studies of the methyltransferase from Clostridium thermoaceticum: Origin of the pH dependence of the methyl group transfer from methyltetrahydrofolate to the corrinoid/iron-sulfur protein

1995

Zhao, S.; Roberts, D.; Ragsdale, S.

Biochemistry

34

15075-15083

720970

21324365

Efficient expression and purification of methyltransferase in acetyl-coenzyme a synthesis pathway of the human pathogen Clostridium difficile

2011

Zhu, X.; Gu, X.; Zhang, S.; Liu, Y.; Huang, Z.; Tan, X.

Protein Expr. Purif.

78

86-93

750412

29809140

Functional and structural characterization of an ECF-type ABC transporter for vitamin B12

2018

Santos, J.A.; Rempel, S.; Mous, S.T.; Pereira, C.T.; Ter Beek, J.; de Gier, J.W.; Guskov, A.; Slotboom, D.J.

eLife

7

e35828

757761

26456915

The thiostrepton A tryptophan methyltransferase TsrM catalyses a cob(II)alamin-dependent methyl transfer reaction

2015

Benjdia, A.; Pierre, S.; Gherasim, C.; Guillot, A.; Carmona, M.; Amara, P.; Banerjee, R.; Berteau, O.

Nat. Commun.

6

8377

Links to other databases for aquacob(III)alamin

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ChEBI

PubChem