Ligand hydroxycobalamin

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Basic Ligand Information

Molecular Structure
Picture of hydroxycobalamin (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C62H92CoN13O15P
hydroxycobalamin
YOZNUFWCRFCGIH-WZHZPDAFSA-L
Synonyms:
aquacob(III)alamin, aquacobalamin, hydroxocobalamin
Pathway Source
Pathways

Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (6 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
aquacobalamin + NADH = reduced aquacobalamin + NAD+
show the reaction diagram
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Product in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
methylcobalamin + tetrahydrofolate = hydroxocobalamin + 5-methyltetrahydrofolate
show the reaction diagram
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Enzyme Cofactor/Cosubstrate (2 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
required for Sbm activity
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Activator in Enzyme-catalyzed Reactions (4 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
stimulates the activity of the purified H2: heterodisulfide oxidoreductase complex
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the addition of 0.05 mM aquacob(III)alamin to the crude extract results in an about 13fold increase in activity which is not greatly affected by the addition of cytochrome P450 reductase or reductase domain of neuronal nitric oxide synthase
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supplementation of hydroxocobalamin results in a marked increase in the holo-MCM activity in a dose-dependent manner, although the holo-MCM activity does not exceed 30% of the total-MCM activity even if hydroxocobalamin is supplemented at 10 mM
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Inhibitor in Enzyme-catalyzed Reactions (5 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
inhibits uptake to a similar extent as cyano-cobalamin
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Enzyme Kinetic Parameters

kcat Value (Turnover Number) (4 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE

KM Value (5 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.0419
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References & Links

Links to other databases for hydroxycobalamin

ChEBI
PubChem
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PubChem