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Ligand alpha-lactose

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)

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Show Natural Substrate (1 entry)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

7.5.2.2

ATP + H2O + lactose/out = ADP + phosphate + lactose/in

289128

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In Vivo Product in Enzyme-catalyzed Reactions (1 result)

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Show Natural Product (1 entry)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

7.5.2.2

ATP + H2O + lactose/out = ADP + phosphate + lactose/in

289128

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Substrate in Enzyme-catalyzed Reactions (8 results)

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Show Substrate (8 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.5.2

alpha-D-lactose + phenazine methosulfate = alpha-D-lactono-1,5-lactone + reduced phenazine methosulfate

746918

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1.1.99.18

alpha-lactose + 2,6-dichlorophenol indophenol = ?

701028

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1.1.99.18

alpha-lactose + acceptor = 4-O-(alpha-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor

287787

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1.1.99.18

2 entries

2.4.99.1

cytidine 5'-monophosphate 3-(axial)-fluoro-N-acetylneuraminic acid + alpha-lactose = ?

702261

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2.4.99.6

CMP-N-acetyl-beta-neuraminate + beta-D-Gal-(1->4)-alpha-D-Glc = CMP + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-alpha-D-Glc

745250

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3.2.1.23

alpha-lactose + H2O = D-galactose + D-glucose

171273

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5.1.3.21

alpha-lactose = beta-lactose

492266

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7.5.2.2

ATP + H2O + lactose/out = ADP + phosphate + lactose/in

289128

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Product in Enzyme-catalyzed Reactions (4 results)

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Show Product (4 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.1.3.23

alpha-D-lactose 1-phosphate + H2O = alpha-D-lactose + phosphate

-

-

3.1.3.23

alpha-lactose 1-phosphate + H2O = alpha-lactose + phosphate

-

-

3.1.3.23

2 entries

5.1.3.3

beta-Lactose = alpha-Lactose

, 2374

-

7.5.2.2

ATP + H2O + lactose/out = ADP + phosphate + lactose/in

289128

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Inhibitor in Enzyme-catalyzed Reactions (1 result)

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Show Inhibitors (1 entry)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

4.2.2.8

-

714553

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3D Structure of Enzyme-Ligand-Complex (PDB) (26 results)

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Show 3D Structure of Enzyme-Ligand-Complex (PDB) (26 entries)

EC NUMBER

ENZYME 3D STRUCTURE

2.4.99.4

2ihz, 3D-view

2.4.99.4

2ilv, 3D-view

2.4.99.4

2ihz, 3D-view

2.4.99.4

2ilv, 3D-view

2.4.99.4

2ihz, 3D-view

2.4.99.4

2ilv, 3D-view

2.4.99.4

2ihz, 3D-view

2.4.99.4

2ilv, 3D-view

2.4.99.4

2ihz, 3D-view

2.4.99.4

2ilv, 3D-view

2.4.99.4

2ihz, 3D-view

2.4.99.4

2ilv, 3D-view

3.2.1.18

1w8o, 3D-view

3.2.1.18

3pjq, 3D-view

3.2.1.18

1w8o, 3D-view

3.2.1.18

3pjq, 3D-view

3.2.1.18

1w8o, 3D-view

3.2.1.18

3pjq, 3D-view

3.2.1.18

1w8o, 3D-view

3.2.1.18

3pjq, 3D-view

3.2.1.18

1w8o, 3D-view

3.2.1.18

3pjq, 3D-view

3.2.1.18

1w8o, 3D-view

3.2.1.18

3pjq, 3D-view

2.4.99.4

12 entries

3.2.1.18

12 entries

3.2.1.63

2eae, 3D-view

4.2.2.1

2yvv, 3D-view

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (5 results)

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Show Turnover Number (5 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

1.1.5.2

225

-

pH 7.5, 70°C, mutant enzyme Y156K

746918

1.1.5.2

282

-

pH 7.5, 70°C, mutant enzyme Y156A

746918

1.1.5.2

1953

-

pH 7.5, 70°C, wild-type enzyme

746918

1.1.99.18

14.22

-

-

701028

1.1.99.18

17

-

-

287787

1.1.5.2

3 entries

1.1.99.18

2 entries

K_M Value (7 results)

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Show KM Value (7 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.1.5.2

0.00019

-

pH 7.5, 70°C, wild-type enzyme

746918

1.1.5.2

0.00064

-

pH 7.5, 70°C, mutant enzyme Y156A

746918

1.1.5.2

0.00065

-

pH 7.5, 70°C, mutant enzyme Y156K

746918

1.1.99.18

0.3846

-

-

701028

1.1.99.18

54

-

-

287787

3.2.1.23

1.9

-

with 0.05 M NaCl

171273

3.2.1.23

7.52

-

without NaCl

171273

1.1.5.2

3 entries

1.1.99.18

2 entries

3.2.1.23

2 entries

References & Links

Literature References (10)

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Show Reference (10 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

2374

7317442

Purification and properties of aldose 1-epimerase from Aspergillus niger

1981

Kinoshita, S.; Kadota, K.; Taguchi, H.

Biochim. Biophys. Acta

662

285-290

171273

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beta-Galactosidase

1960

Wallenfels, K.; Malhotra, O.M.

The Enzymes, 2nd Ed (Boyer, P. D. , Lardy, H. , Myrbäck, K. , eds. )

4

409-430

287787

11282631

Purification and characterization of cellobiose dehydrogenase from the plant pathogen Sclerotium (Athelia) rolfsii

2001

Baminger, U.; Subramaniam, S.S.; Renganathan, V.; Halterich, d.

Appl. Environ. Microbiol.

67

1766-1774

289128

1630315

Molecular analysis of the lac operon encoding the binding-protein-dependent lactose transport system and beta-galactosidase in Agrobacterium radiobacter

1992

Williams, S.G.; Greenwood, J.A.; Jones, C.W.

Mol. Microbiol.

6

1755-1768

492266

7796915

A novel enzyme, maltose 1-epimerase from Lactobacillus brevis IFO 3345

1995

Shirokane, Y.; Suzuki, M.

FEBS Lett.

367

177-179

701028

-

Cellobiose dehydrogenase production by the mycelial culture of the mushroom Termitomyces clypeatus

2008

Saha, T.; Ghosh, D.; Mukherjee, S.; Bose, S.; Mukherjee, M.

Process Biochem.

43

634-641

702261

19845399

Nuclear magnetic resonance structural characterization of substrates bound to the alpha-2,6-sialyltransferase, ST6Gal-I

2009

Liu, S.; Meng, L.; Moremen, K.W.; Prestegard, J.H.

Biochemistry

48

11211-11219

714553

21445884

Biochemical analysis and kinetic modeling of the thermal inactivation of MBP-fused heparinase I: implications for a comprehensive thermostabilization strategy

2011

Chen, S.; Ye, F.; Chen, Y.; Chen, Y.; Zhao, H.; Yatsunami, R.; Nakamura, S.; Arisaka, F.; Xing, X.H.

Biotechnol. Bioeng.

108

1841-1851

745250

7204397

Detection of beta-galactosyl(1->4)N-acetylglucosaminide alpha(2->3)-sialyltransferase activity in fetal calf liver and other tissues

1981

Van den Eijnden, D.H.; Schiphorst, W.E.C.M.

J. Biol. Chem.

256

3159-3162

746918

27592307

Biochemical and structural characterization of quinoprotein aldose sugar dehydrogenase from Thermus thermophilus HJ6 Mutational analysis of Tyr156 in the substrate-binding site

2016

Kim, H.; Wang, J.; Lee, J.; Park, A.; Park, H.; Jeon, S.

Arch. Biochem. Biophys.

608

20-26

Links to other databases for alpha-lactose

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Show Links (1 entry)

ChEBI

PubChem

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