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0.05
-
pH 7.3, 23°C, conversion to compound 5
0.2
-
pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate
0.4
-
below, pH 7.3, 23°C, mutant R11A/R39A
0.6
-
pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate
0.6
-
pH 7.3, 23°C, conversion to compound 5
2
8
pH 7.3, 23°C, mutant R39A
3
6
pH 7.3, 24°C, recombinant hh4-OT mutant betaP1A
9
-
pH 7.3, 23°C, mutant R39Q
40
-
pH 7.3, 23°C, mutant R11A
43
-
pH 7.3, 24°C, recombinant hh4-OT mutant alphaR12A
65
-
pH 7.3, 24°C, recombinant hh4-OT mutant alphaR40A
733
-
pH 7.3, 24°C, recombinant hh4-OT mutant betaR39A
1330
-
at pH 7.3 and 22°C
3000
-
pH 7.3, 24°C, recombinant wild-type hh4-OT
3500
-
pH 7.3, 23°C, wild-type enzyme
3500
-
pH 7.3, 24°C, recombinant hh4-OT mutant betaR11A
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0.017
-
pH 7.3, 24°C, recombinant hh4-OT mutant betaP1A
0.05
-
at pH 7.3 and 22°C
0.069
-
pH 7.3, 24°C, recombinant hh4-OT mutant betaR11A
0.07
-
pH 7.3, 24°C, recombinant wild-type hh4-OT
0.11
-
pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate
0.11
-
pH 7.3, 23°C, conversion to compound 5
0.135
-
pH 7.3, 23°C, conversion to compound 5
0.135
-
pH 7.3, 24°C, recombinant hh4-OT mutant betaR39A
0.17
-
pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate
0.18
-
pH 7.3, 23°C, wild-type enzyme
0.29
-
pH 7.3, 23°C, mutant R11A/R39A
0.29
-
pH 7.3, 23°C, mutant R39A
0.345
-
pH 7.3, 24°C, recombinant hh4-OT mutant alphaR40A
0.47
-
pH 7.3, 23°C, mutant R39Q
1.033
-
pH 7.3, 24°C, recombinant hh4-OT mutant alphaR12A
1.6
-
pH 7.3, 23°C, mutant R11A
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Kinetic, stereochemical, and structural effects of mutations of the active site arginine residues in 4-oxalocrotonate tautomerase
1999
Harris, T.K.; Czerwinski, R.M.; Johnson, W.H.; Legler, P.M.; Abeygunawardana, C.; Massiah, M.A.; Stivers, J.T.; Whitman, C.P.; Mildvan, A.S.
Biochemistry
38
12343-12357
Kinetic and stereochemical analysis of YwhB, a 4-oxalocrotonate tautomerase homologue in Bacillus subtilis: Mechanistic implications for the YwhB- and 4-oxalocrotonate tautomerase-catalyzed reactions
2007
Wang, S.; Johnson Jr., W.; Czerwinski, R.; Stamps, S.; Whitman, C.
Biochemistry
46
11919-11929
Kinetic and structural characterization of a heterohexamer 4-oxalocrotonate tautomerase from Chloroflexus aurantiacus J-10-fl: implications for functional and structural diversity in the tautomerase superfamily
2010
Burks, E.A.; Fleming, C.D.; Mesecar, A.D.; Whitman, C.P.; Pegan, S.D.
Biochemistry
49
5016-5027
Kinetic and structural characterization of DmpI from Helicobacter pylori and Archaeoglobus fulgidus, two 4-oxalocrotonate tautomerase family members
2010
Almrud, J.J.; Dasgupta, R.; Czerwinski, R.M.; Kern, A.D.; Hackert, M.L.; Whitman, C.P.
Bioorg. Chem.
38
252-259
Structural and kinetic characterization of two 4-oxalocrotonate tautomerases in Methylibium petroleiphilum strain PM1
2013
Terrell, C.R.; Burks, E.A.; Whitman, C.P.; Hoffman, D.W.
Arch. Biochem. Biophys.
537
113-124
Kinetic, crystallographic, and mechanistic characterization of TomN: elucidation of a function for a 4-oxalocrotonate tautomerase homologue in the tomaymycin biosynthetic pathway
2011
Burks, E.A.; Yan, W.; Johnson, W.H.; Li, W.; Schroeder, G.K.; Min, C.; Gerratana, B.; Zhang, Y.; Whitman, C.P.
Biochemistry
50
7600-7611
Highly efficient Michael-type addition of acetaldehyde to beta-nitrostyrenes by whole resting cells of Escherichia coli expressing 4-oxalocrotonate tautomerase
2013
Narancic, T.; Radivojevic, J.; Jovanovic, P.; Francuski, D.; Bigovic, M.; Maslak, V.; Savic, V.; Vasiljevic, B.; O'Connor, K.E.; Nikodinovic-Runic, J.
Biores. Technol.
142
462-468
Promiscuous catalysis of asymmetric Michael-type additions of linear aldehydes to beta-nitrostyrene by the proline-based enzyme 4-oxalocrotonate tautomerase
2013
Miao, Y.; Geertsema, E.M.; Tepper, P.G.; Zandvoort, E.; Poelarends, G.J.
ChemBioChem
14
191-194
Biocatalytic Michael-type additions of acetaldehyde to nitroolefins with the proline-based enzyme 4-oxalocrotonate tautomerase yielding enantioenriched gamma-nitroaldehydes
2013
Geertsema, E.M.; Miao, Y.; Tepper, P.G.; de Haan, P.; Zandvoort, E.; Poelarends, G.J.
Chemistry
19
14407-14410
Identification and characterization of new family members in the tautomerase superfamily analysis and implications
2014
Huddleston, J.P.; Burks, E.A.; Whitman, C.P.
Arch. Biochem. Biophys.
564
189-196
Inactivation of 4-oxalocrotonate tautomerase by 5-halo-2-hydroxy-2,4-pentadienoates
2018
Stack, T.M.M.; Li, W.; Johnson, W.H.; Zhang, Y.J.; Whitman, C.P.
Biochemistry
57
1012-1021
Immobilization of Escherichia coli cells expressing 4-oxalocrotonate tautomerase for improved biotransformation of beta-nitrostyrene
2015
Djokic, L.; Spasic, J.; Jeremic, S.; Vasiljevic, B.; Prodanovic, O.; Prodanovic, R.; Nikodinovic-Runic, J.
Bioprocess Biosyst. Eng.
38
2389-2395
Immobilization of Escherichia coli cells expressing 4-oxalocrotonate tautomerase for improved biotransformation of beta-nitrostyrene
2015
Djokic, L.; Spasic, J.; Jeremic, S.; Vasiljevic, B.; Prodanovic, O.; Prodanovic, R.; Nikodinovic-Runic, J.
Bioprocess Biosyst. Eng.
38
2389-2395
Evidence for the formation of an enamine species during aldol and Michael-type addition reactions promiscuously catalyzed by 4-oxalocrotonate tautomerase
2015
Poddar, H.; Rahimi, M.; Geertsema, E.M.; Thunnissen, A.M.; Poelarends, G.J.
ChemBioChem
16
738-741
Demethionylation of Pro-1 variants of 4-oxalocrotonate tautomerase in Escherichia coli by co-expression with an engineered methionine aminopeptidase
2014
Baas, B.J.; Zandvoort, E.; Wasiel, A.A.; Poelarends, G.J.
FEBS Open Bio
4
651-658
Demethionylation of Pro-1 variants of 4-oxalocrotonate tautomerase in Escherichia coli by co-expression with an engineered methionine aminopeptidase
2014
Baas, B.J.; Zandvoort, E.; Wasiel, A.A.; Poelarends, G.J.
FEBS Open Bio
4
651-658
-
Importance of N-terminal proline for the promiscuous activity of 4-oxalocrotonate tautomerase (4-OT)
2016
Lazic, J.; Spasic, J.; Francuski, D.; Tokic-Vujosevic, Z.; Nikodinovic-Runic, J.; Maslak, V.; Djokic, L.
J. Serb. Chem. Soc.
81
871-881
Using mutability landscapes of a promiscuous tautomerase to guide the engineering of enantioselective Michaelases
2016
van der Meer, J.Y.; Poddar, H.; Baas, B.J.; Miao, Y.; Rahimi, M.; Kunzendorf, A.; van Merkerk, R.; Tepper, P.G.; Geertsema, E.M.; Thunnissen, A.M.; Quax, W.J.; Poelarends, G.J.
Nat. Commun.
7
10911
-
Synthesis of gamma-nitroaldehydes containing quaternary carbon in the alpha-position using a 4-oxalocrotonate tautomerase whole-cell biocatalyst
2014
Radivojevic, J.; Minovska, G.; Senerovic, L.; OConnor, K.; Jovanovic, P.; Savic, V.; Tokic-Vujosevic, Z.; Nikodinovic-Runic, J.; Maslak, V.
RSC Adv.
4
60502-60510
The S. aureus 4-oxalocrotonate tautomerase SAR1376 enhances immune responses when fused to several antigens
2017
Diemen, P.M.V.; Leneghan, D.B.; Brian, I.J.; Miura, K.; Long, C.A.; Milicic, A.; Biswas, S.; Rollier, C.S.; Wyllie, D.H.
Sci. Rep.
7
1745
The S. aureus 4-oxalocrotonate tautomerase SAR1376 enhances immune responses when fused to several antigens
2017
Diemen, P.M.V.; Leneghan, D.B.; Brian, I.J.; Miura, K.; Long, C.A.; Milicic, A.; Biswas, S.; Rollier, C.S.; Wyllie, D.H.
Sci. Rep.
7
1745
Substituting the catalytic proline of 4-oxalocrotonate tautomerase with non-canonical analogues reveals a finely tuned catalytic system
2019
Lukesch, M.S.; Pavkov-Keller, T.; Gruber, K.; Zangger, K.; Wiltschi, B.
Sci. Rep.
9
2697
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