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Ligand N-acetyl-Phe-tRNA

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)

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Show Natural Substrate (1 entry)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.3.2.6

L-phenylalanyl-tRNA(Leu) + L-arginyl-peptide = tRNA(Leu) + L-phenylalanyl-L-arginyl-protein

720253

-

Substrate in Enzyme-catalyzed Reactions (5 results)

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Show Substrate (5 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.3.2.12

acetylphenylalanyl-tRNA + puromycin = acetylphenylalanyl-puromycin + tRNA

487799

-

2.3.2.12

N-AcPhe-tRNA + puromycin = tRNA + N-AcPhe-puromycin

487803, 675409

-

2.3.2.12

2 entries

2.3.2.6

L-phenylalanyl-tRNA(Leu) + L-arginyl-peptide = tRNA(Leu) + L-phenylalanyl-L-arginyl-protein

720253

-

3.1.1.29

N-acetyl-Phe-tRNA + H2O = N-acetyl-L-Phe + tRNA

648116

-

3.1.1.29

N-acetyl-Phe-tRNA + H2O = N-acetyl-Phe + tRNA

648116, 648113, 648114, 648106, 648107, 648111

-

3.1.1.29

2 entries

Enzyme Kinetic Parameters

K_M Value (3 results)

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Show KM Value (3 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.3.2.12

0.000058

-

-

487799

3.1.1.29

0.000001

-

pH 7.0, 30°C

648107

3.1.1.29

0.0008

-

pH 7.5, 30°C

648114

3.1.1.29

2 entries

References & Links

Literature References (10)

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Show Reference (10 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

487799

4819752

Characterization of the peptidyltransferase reaction catalyzed by rat liver 60S ribosomal subunits

1974

Thompson, H.A.; Moldave, K.

Biochemistry

13

1348-1353

487803

358982

Inhibition of ribosomal peptidyltransferase with cytidyl-3 leads to 5-[2(3)-O-L-phenylalanyl]-L-adenosine

1978

Bhuta, P.; Zemlicka, J.

Biochem. Biophys. Res. Commun.

83

414-420

648106

11945282

Enzymatic hydrolysis of N-substituted aminoacyl-tRNA

1968

Paulin, D.; Yot, P.; Chapeville, F.

FEBS Lett.

1

163-165

648107

4981785

Enzymatic hydrolysis of N-substituted aminoacyl transfer ribonucleic acid in yeast

1969

Jost, J.P.; Bock, R.M.

J. Biol. Chem.

244

5866-5873

648111

247994

Independent temporal expression of two N-substituted aminoacyl-tRNA hydrolases during the development of Artemia salina

1978

Miralles, J.; Sebastian, J.; Heredia, C.F.

Biochim. Biophys. Acta

518

326-333

648113

-

Purification and properties of an inducible aminoacyl-tRNA hydrolase from Artemia larvae

1982

Gallego, M.E.; Heredia, C.F.

Biochim. Biophys. Acta

696

57-65

648114

-

Purification and characterization of an aminoacyl-tRNA hydrolase from the filamentous fungus Fusarium culmorum

1986

Ferreiro, C.; Heredia, C.F.

Biochim. Biophys. Acta

882

410-418

648116

12475929

Orthologs of a novel archaeal and of the bacterial peptidyl-tRNA hydrolase are nonessential in yeast

2002

Rosas-Sandoval, G.; Amrogelly, A.; Rinehart, J.; Wei, D.; Cruz-Vera, L.R.; Graham, D.E.; Stetter, K.O.; Guarneros, G.; Soell, D.

Proc. Natl. Acad. Sci. USA

99

16707-16712

675409

17045291

A conserved base-pair between tRNA and 23 S rRNA in the peptidyl transferase center is important for peptide release

2006

Feinberg, J.S.; Joseph, S.

J. Mol. Biol.

364

1010-1020

720253

21530538

An alternative mechanism for the catalysis of peptide bond formation by L/F transferase: substrate binding and orientation

2011

Fung, A.W.; Ebhardt, H.A.; Abeysundara, H.; Moore, J.; Xu, Z.; Fahlman, R.P.

J. Mol. Biol.

409

617-629

Links to other databases for N-acetyl-Phe-tRNA

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Show Links (1 entry)