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Ligand 3,12-Dioxo-5beta-cholanoate

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)

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Show Natural Substrate (1 entry)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.391

3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+ = 3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+

735471

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Substrate in Enzyme-catalyzed Reactions (6 results)

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Show Substrate (6 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.176

5beta-cholanic acid-3,12-dione + NADPH + H+ = 3-oxodeoxycholic acid + NADP+

760391

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1.1.1.238

3,12-dioxo-5beta-cholanoate + NADPH = 12beta-hydroxy-3-oxo-5beta-cholanoate + NADP+

286353

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1.1.1.391

3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+ = 3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+

735471

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1.1.1.391

3,12-dioxo-5beta-cholan-24-oic acid + NADH + H+ = 3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+

286228, 735471

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1.3.99.6

3,12-dioxo-5beta-cholan-24-oic acid + menaphthone = 3,12-dioxo-4-cholen-24-oic acid + reduced menaphthone

391381

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1.3.99.6

3,12-dioxo-5beta-cholan-24-oic acid + phenazine methosulfate = 3,12-dioxo-4-cholen-24-oic acid + reduced phenazine methosulphate

391381

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1.1.1.391

2 entries

1.3.99.6

2 entries

Product in Enzyme-catalyzed Reactions (3 results)

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Show Product (3 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.159

dehydrocholic acid + NADH = 3,12-diketolithocholic acid + NAD+

286075

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3.5.1.24

3,12-dioxo-5beta-cholanoylglycine + H2O = 3,12-dioxo-5beta-cholanate + Gly

0

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3.5.1.24

3,12-dioxo-5beta-cholanoyltaurine + H2O = 3,12-dioxo-5beta-cholanate + taurine

0

-

3.5.1.24

2 entries

Enzyme Kinetic Parameters

K_M Value (1 result)

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Show KM Value (1 entry)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.1.1.238

0.0895

-

-

286353

References & Links

Literature References (6)

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Show Reference (6 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

286075

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Purification and properties of three kinds of alpha-hydroxysteroid dehydrogenase from Brevibacterium fuscum DC33

1988

Kinoshita, S.; Kadota, K.; Inoue, T.; Sawada, H.; Taguchi, H.

J. Ferment. Technol.

66

145-152

286228

2752021

Characterization of NAD-dependent 3alpha- and 3beta-hydroxysteroid dehydrogenase and of NADP-dependent 7beta-hydroxysteroid dehydrogenase from Peptostreptococcus productus

1989

Edenharder, R.; Pfutzner, A.; Hammann, R.

Biochim. Biophys. Acta

1004

230-238

286353

3167086

Characterization of NADP-dependent 12beta-hydroxysteroid dehydrogenase from Clostridium paraputrificum

1988

Edenharder, R.; Pfutzner, A.

Biochim. Biophys. Acta

962

362-370

391381

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Degradation of steroids by intestinal bacteria. III. 3-Oxo-5beta-steroid DELTA1-dehydrogenase and 3-oxo-5beta-steroid DELTA4-dehydrogenase

1971

Aries, V.C.; Goddard, P.; Hill, M.J.

Biochim. Biophys. Acta

248

482-488

735471

2764572

Partial purification and characterization of an NAD-dependent 3beta-hydroxysteroid dehydrogenase from Clostridium innocuum

1989

Edenharder, R.; Pfuetzner, M.

Appl. Environ. Microbiol.

55

1656-1659

760391

29549099

Metabolism of oxo-bile acids and characterization of recombinant 12alpha-hydroxysteroid dehydrogenases from bile acid 7alpha-dehydroxylating human gut bacteria

2018

Doden, H.; Sallam, L.A.; Devendran, S.; Ly, L.; Doden, G.; Daniel, S.L.; Alves, J.M.P.; Ridlon, J.M.

Appl. Environ. Microbiol.

84

e00235-18

Links to other databases for 3,12-Dioxo-5beta-cholanoate

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Show Links (1 entry)

ChEBI

PubChem

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