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0.9
-
recombinant mutant F54S, pH 7.5, 22°C
1
-
mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
1
-
mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
1.2
-
recombinant mutant F54A, pH 7.5, 22°C
1.6
-
recombinant wild-type enzyme, pH 7.5, 22°C
1.7
-
mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
1.9
-
mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
1.9
-
mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
1.9
-
pH 8.5, 25°C, mutant enzyme Y238F
1.9
-
pH 8.5, 25°C, mutant enzyme Y238S
2.5
-
mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
2.8
-
mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
3.8
-
mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
4.3
-
mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
4.8
-
recombinant mutant F54Y, pH 7.5, 22°C
5
-
immobilized enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25°C
5
-
pH 8.5, 25°C, wild-type enzyme
5
-
wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
6
-
free enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25°C
6
-
immobilized enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25°C
6
-
native enzyme, at 37°C
8.7
-
mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
9
-
free enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25°C
9
-
native enzyme, at 37°C
13
-
double fusion enzyme, at 37°C
13
-
mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
20
-
double fusion enzyme, at 37°C
28
-
mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
119
-
free enzyme, at pH 8.0 and 30°C
174.7
-
immobilized cross-linked enzyme aggregate, at pH 8.0 and 30°C
34.5
-
recombinant enzyme
215
-
pH 7.3, 25°C, recombinant enzyme
1.5
-
recombinant mutant H57betaS/H70betaS/F58betaD, pH 8.0, 37°C
1.5
-
recombinant mutant H57betaS/H70betaS/L176betaC, pH 8.0, 37°C
1.7
-
pH 8.0, 37°C, mutant enzyme H296S/H417M
1.8
-
recombinant mutant H57betaS/H70betaS/M165alphaS/I176betaS, pH 8.0, 37°C
2
-
recombinant mutant H57betaS/H70betaS/R24betaP, pH 8.0, 37°C
2.3
-
recombinant mutant H57betaS/H70betaS/V25betaR, pH 8.0, 37°C
2.7
-
pH 8.0, 37°C, mutant enzyme Y271V
3.1
-
recombinant mutant H57betaS/H70betaS/L154betaY/M165alphaS, pH 8.0, 37°C
3.2
-
pH 8.0, 37°C, mutant enzyme A215E
3.8
-
recombinant mutant H57betaS/H70betaS/L175betaT, pH 8.0, 37°C
4.1
-
pH 8.0, 37°C, mutant enzyme H296F
4.5
-
pH 8.0, 25°C, recombinant mutant H57betaS/H70betaS/L154betaY
4.5
-
recombinant mutant H57betaS/H70betaS/L154betaY, pH 8.0, 37°C
4.8
-
pH 8.0, 37°C, mutant enzyme H296T
5.2
-
pH 8.0, 37°C, mutant enzyme H296N
5.4
-
recombinant mutant H57betaS/H70betaS/L176betaT, pH 8.0, 37°C
6.3
-
pH 8.0, 37°C, mutant enzyme H309Y
6.5
-
pH 8.0, 37°C, mutant enzyme F270M
6.7
-
pH 8.0, 37°C, mutant enzyme H296S
6.9
-
pH 8.0, 37°C, mutant enzyme A215Y
7.2
-
pH 8.0, 37°C, mutant enzyme D416Y
7.5
-
recombinant mutant H57betaS/H70betaS/L176betaK, pH 8.0, 37°C
7.7
-
pH 8.0, 37°C, mutant enzyme A215F
8
-
pH 8.0, 37°C, mutant enzyme Y271F
8.1
-
recombinant enzyme mutant M6, pH and temperature not specified in the publication
8.3
-
pH 8.0, 37°C, mutant enzyme A215V
9.3
-
recombinant mutant L154betaF/L180betaF, pH 8.0, 37°C
9.5
-
pH 8.0, 25°C, recombinant wild-type enzyme
9.5
-
pH 8.0, 37°C, mutant enzyme A215L
9.5
-
recombinant mutant wild-type enzyme, pH 8.0, 25°C
9.5
-
recombinant wild-type enzyme, pH 8.0, 25°C
9.5
-
wild type enzyme, at pH 8.0 and 25°C
9.73
-
recombinant mutant L154betaF, pH 8.0, 37°C
10
-
pH 8.0, 37°C, mutant enzyme A215Y/H296S
10.1
-
pH 8.0, 37°C, mutant enzyme Y215Y/F270S
10.14
-
recombinant mutant L180betaF, pH 8.0, 37°C
11
-
pH 8.0, 37°C, wild-type enzyme
11
-
recombinant wild-type enzyme, pH and temperature not specified in the publication
12.2
-
mutant enzyme H296S/H309S, at pH 8.0 and 25°C
12.2
-
pH 8.0, 25°C, recombinant mutant H57betaS/H70betaS
12.2
-
pH 8.0, 37°C, mutant enzyme H296S/H309S
12.2
-
recombinant mutant H57betaS/H70betaS, pH 8.0, 25°C
12.3
-
pH 8.0, 37°C, mutant enzyme H417Y
12.38
-
recombinant mutant M31betaF/H57betaS/V68betaA/H70betaS, pH 8.0, 37°C
12.65
-
recombinant mutant M31betaF/H57betaS/H70betaS, pH 8.0, 37°C
13
-
pH 8.0, 37°C, mutant enzyme D416Y/H417Y
16.4
-
pH 8.0, 25°C, recombinant mutant H57betaS/H70betaS/F72betaR
16.4
-
recombinant mutant H57betaS/H70betaS/F72betaR, pH 8.0, 37°C
17.2
-
pH 8.0, 37°C, mutant enzyme A215Y/H309S
17.8
-
pH 8.0, 37°C, mutant enzyme A215Y/H296S/H309S
18.9
-
pH 8.0, 37°C, mutant enzyme E89A/A215Y
19.2
-
recombinant free enzyme, pH 8.5, 37°C
21.3
-
37°C, mutant enzyme Y269F
21.6
-
37°C, wild-type enzyme
22
-
at 37°C, pH not specified in the publication
22.1
-
37°C, mutant enzyme Y269Y
24.4
-
pH 8.0, 37°C, mutant enzyme H309S
24.5
-
pH 8.0, 37°C, mutant enzyme S22P/T394P/D416Y/H417Y
27.7
-
recombinant mutant H57betaS/H70betaS/M165alphaS, pH 8.0, 37°C
27.7
-
recombinant mutant M165alphaS/H57betaS/H70betaS, pH 8.0, 25°C
27.8
-
37°C, mutant enzyme Y270F
32
-
pH 8.0, 37°C, mutant enzyme I44V/E49stop/D416Y/H417Y
33.7
-
recombinant mutant H57betaS/H70betaS/F58betaI, pH 8.0, 37°C
37
-
mutant enzyme L677F, in 100 mM Tris-HCl buffer, pH 8.0, temperature not specified in the publication
37
-
pH 8.5, 37°C, recombinant mutant L677F
43
-
pH 8.5, 37°C, recombinant wild-type enzyme
43
-
wild type enzyme, in 100 mM Tris-HCl buffer, pH 8.0, temperature not specified in the publication
51
-
deletion mutant D4(212-ADLA-215), pH 8.5, 37°C
51.6
-
wild-type enzyme, pH 8.5, 37°C
74.8
-
deletion mutant D2(227-AM-228), pH 8.5, 37°C
86
-
mutant enzyme P295A, in 100 mM Tris-HCl buffer, pH 8.0, temperature not specified in the publication
86
-
pH 8.5, 37°C, recombinant mutant P295A
95.1
-
recombinant immobilized enzyme, pH 8.5, 37°C
105
-
mutant enzyme A675G, in 100 mM Tris-HCl buffer, pH 8.0, temperature not specified in the publication
105
-
pH 8.5, 37°C, recombinant mutant A675G
121
-
mutant enzyme H309V, in 100 mM Tris-HCl buffer, pH 8.0, temperature not specified in the publication
121
-
pH 8.5, 37°C, recombinant mutant H309V
0.114
-
in 100 mM MES (pH 6.4)
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Isolation, analysis, and expression of two genes from Thermoanaerobacterium sp. strain JW/SL YS485: a beta-xylosidase and a novel acetyl xylan esterase with cephalosporin C deacetylase activity
1997
Lorenz, W.W.; Wiegel, J.
J. Bacteriol.
179
5436-5441
Enzymatic hydrolysis of cephalosporin C by an extracellular acetylhydrolase of Cephalosporium acremonium
1976
Hinnem, A.; Nuesch, J.
Antimicrob. Agents Chemother.
9
824-830
Cephalosporin acetylesterase (Bacillus subtilis)
1978
Abbott, B.J.; Fukuda, D.S.
Methods Enzymol.
43
731-734
Cephalosporin acetylesterase (citrus)
1975
Abraham, E.P.; Fawcett, P.
Methods Enzymol.
43
728-731
-
Deacetylcephalosporin C formation by cephalosporin C acetylhydrolase induced in a Cephalosporium acremonium
1975
Fujisawa, Y.; Shirafuji, H.; Kanzaki, T.
Agric. Biol. Chem.
39
1303-1309
-
A biochemical and genetical approach to the biosynthesis of cephalosporin C
1976
Nuesch, J.; Hinnen, A.; Liersch, M.; Treichler, H.J.
Proc. Int. Symp. Genet. Ind. Microorg. (McDonald, K. D. , ed. )
-
451-472
New findings on cephalosporin C biosynthesis
1973
Fujisawa, Y.; Shirafuji, H.; Kida, M.; Nara, K.
Nature New Biol.
246
154-155
-
Purification and properties of cephalosporin-C deacetylase from the yeast, Rhodotorula glutinis 38B1, useful for bioconversion of 7-aminocephalosporanic acid derivatives
1998
Sakai, Y.; Abe, T.; Aoki, M.; Ohabayashi, Y.; Yamamoto, K.; Tani, Y.; Kato, N.
J. Ferment. Bioeng.
85
53-57
-
Purification, characterization and partial amino acid sequences of a novel cephalosporin-C deacetylase from Bacillus subtilis
1994
Takimoto, A.; Mitsushima, K.; Yagi, S.; Sonoyama, T.
J. Ferment. Bioeng.
77
17-22
Purification and characterization of a cephalosporin esterase from Phodosporidium toruloides
1997
Politino, M.; Tonzi, S.M.; Burnett, W.V.; Romancik, G.; Usher, J.J.
Appl. Environ. Microbiol.
63
4807-4811
High-level expression, purification, and some properties of a recombinant cephalosporin-C deacetylase
1999
Takimoto, A.; Yagi, S.; Mitsushima, K.
J. Biosci. Bioeng.
87
456-462
Purification and properties of beta-lactamase from Proteus morganii
1977
Fujii-Kuriyama, Y.; Yamamoto, M.; Sugawara, S.
J. Bacteriol.
131
726-734
beta-Lactamases (Actinomycetes species)
1975
Johnson, K.; Duez, C.; Frere, J.M.; Ghuysen, J.M.
Methods Enzymol.
43
687-698
beta-Lactamase (Enterobacter species)
1975
Ross, G.W.
Methods Enzymol.
43
678-687
-
beta-Lactamase (Bacillus licheniformis)
1975
Thatcher, D.R.
Methods Enzymol.
43
652-664
beta-Lactamase (Bacillus cereus)
1975
Thatcher, D.R.
Methods Enzymol.
43
640-652
Purification and properties of a constitutive beta-lactamase from Pseudomonas aeruginosa strain Dalgleish
1975
Furth, A.J.
Biochim. Biophys. Acta
377
431-443
Purification of beta-lactamases on QAE-sephadex
1973
Ross, G.W.; Boulton, M.G.
Biochim. Biophys. Acta
309
430-439
Effects on substrate profile by mutational substitutions at position 164 and 179 of the class A TEMpUC19beta-lactamase from Escherichia coli
1999
Vakulenko, S.B.; Taibi-Tronche, P.; Toth, M.; Massova, I.; Lerner, S.A.; Mobashery, S.
J. Biol. Chem.
274
23052-23060
Overexpression, purification, and characterization of the cloned metallo-beta-lactamase L1 from Stenotrophomonas maltophilia
1998
Crowder, M.W.; Walsh, T.R.; Banovic, L.; Pettit, M.; Spencer, J.
Antimicrob. Agents Chemother.
42
921-926
-
A comparative study of beta-lactamases
1993
Ledent, P.; Raquet, X.; Joris, B.; Van Beeumen, J.; Frere, J.M.
Biochem. J.
292
439-443
-
Site-directed mutagenesis and substrate-induced inactivation of beta-lactamase I
1992
Thornewell, J.; Waley, G.
Biochem. J.
288
1045-1051
Characterization of cell-bound papain-soluble beta-lactamases in BRO-1 and BRO-2 producing strains of Moraxella (Branhamella) catarrhalis and Moraxella nonliquefaciens
1992
Eliasson, I.; Kamme, C.; Vang, M.; Waley, S.G.
Eur. J. Clin. Microbiol. Infect. Dis.
11
313-321
-
Isolation and partial characterization of a D-amino acid oxidase active against cephalosporin. c from the yeast Trigonopsis variabilis
1985
Szwajcer, E.; Mosbach, K.
Biotechnol. Lett.
7
1-7
-
Properties and chemical modification of D-amino acid oxidase from Trigonopsis variabilis
1996
Schraeder, T.; Andreesen, J.R.
Arch. Microbiol.
165
41-47
Redox potentials and their pH dependence of D-amino-acid oxidase of Rhodotorula gracilis and Trigonopsis variabilis
2000
Pollegioni, L.; Porrini, D.; Molla, G.; Pilone, M.S.
Eur. J. Biochem.
267
6624-6632
D-Amino acid oxidase: new findings
2000
Pilone, M.S.
Cell. Mol. Life Sci.
57
1732-1747
Stabilization of D-amino-acid oxidase from Trigonopsis variabilis by manganese dioxide
1999
Vikartovska-Welwardova, A.; Michalkova, E.; Gemeiner, P.; Welward, L.
Folia Microbiol. (Praha)
44
380-384
Characterization of D-amino acid oxidase from Trigonopsis variabilis
1993
Pollegioni, L.; Buto, S.; Tischer, W.; Ghisla, S.; Pilone, M.S.
Biochem. Mol. Biol. Int.
31
709-717
Novel D-amino acid transaminase
1988
Aretz, W.; Sauber, K.
Ann. N. Y. Acad. Sci.
542
366-370
Purification, crystallization and preliminary X-ray analysis of an acetylxylan esterase from Bacillus pumilus
2001
Benini, S.; Degrassi, G.; Krastanova, I.; Lamba, D.; Venturi, V.
Acta Crystallogr. Sect. D
57
1906-1907
A cephalosporin C acetylhydrolase is present in the cultures of Nocardia lactamdurans
2000
Cardoza, R.E.; Velasco, J.; Martin, J.F.; Liras, P.
Appl. Microbiol. Biotechnol.
54
406-412
Cloning and characterization of the gene cahB encoding a cephalosporin C acetylhydrolase from Acremonium chrysogenum
2001
Velasco, J.; Gutierrez, S.; Casqueiro, J.; Fierro, F.; Campoy, S.; Martin, J.F.
Appl. Microbiol. Biotechnol.
57
350-356
-
Isolation, analysis, and expression of lipase with cephalosporin-C deacetylation activity from Staphylococcus sp
2001
Lee, H.W.; Ko, J.Y.; Kim, W.J.; Byun, S.M.
J. Biochem. Mol. Biol.
34
274-277
Multifunctional xylooligosaccharide/cephalosporin C deacetylase revealed by the hexameric structure of the Bacillus subtilis enzyme at 1.9A resolution
2003
Vincent, F.; Charnock, S.J.; Verschueren, K.H.; Turkenburg, J.P.; Scott, D.J.; Offen, W.A.; Roberts, S.; Pell, G.; Gilbert, H.J.; Davies, G.J.; Brannigan, J.A.
J. Mol. Biol.
330
593-606
D-Amino acid oxidase: structure, catalytic mechanism, and practical application
2005
Tishkov, V.I.; Khoronenkova, S.V.
Biochemistry
70
40-54
Use of physicochemical tools to determine the choice of optimal enzyme: stabilization of D-amino acid oxidase
2003
Betancor, L.; Hidalgo, A.; Fernandez-Lorente, G.; Mateo, C.; Rodriguez, V.; Fuentes, M.; Lopez-Gallego, F.; Fernandez-Lafuente, R.; Guisan, J.M.
Biotechnol. Prog.
19
784-788
Catalytic properties of D-amino acid oxidase in cephalosporin C bioconversion: a comparison between proteins from different sources
2004
Pollegioni, L.; Caldinelli, L.; Molla, G.; Sacchi, S.; Pilone, M.S.
Biotechnol. Prog.
20
467-473
Role of tyrosine 238 in the active site of Rhodotorula gracilis D-amino acid oxidase. A site-directed mutagenesis study
2002
Boselli, A.; Sacchi, S.; Job, V.; Pilone, M.S.; Pollegioni, L.
Eur. J. Biochem.
269
4762-4771
Scintillation proximity assay for inhibitors of Escherichia coli MurG and, optionally, MraY
2005
Ravishankar, S.; Kumar, V.P.; Chandrakala, B.; Jha, R.K.; Solapure, S.M.; De Sousa, S.M.
Antimicrob. Agents Chemother.
49
1410-1418
-
Industrial transformation of penicillins and cephalosporins
1993
Verweij, J.; Vroom, E.D.
Rec. Trav. Chim. Pays-Bas
112
66-81
Protein engineering of a cephalosporin C acylase
1996
Yamada, H.; Ishii, Y.; Noguchi, Y.; Miura, T.; Mori, T.; Saito, Y.
Ann. N. Y. Acad. Sci.
799
74-81
Biochemical characterization of a glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas strain BL072
1994
Binder, R.; Brown, J.; Romancik, G.
Appl. Environ. Microbiol.
60
1805-1809
Oxidative modification of a cephalosporin C acylase from Pseudomonas strain N176 and site-directed mutagenesis of the gene
1996
Saito, Y.; Fujimura, T.; Ishii, Y.; Noguchi, Y.; Miura, T.; Niwa, M.; Shimomura, K.
Appl. Environ. Microbiol.
62
2919-2925
Improving the activity and stability of GL-7-ACA acylase CA130 by site-directed mutagenesis
2005
Zhang, W.; Liu, Y.; Zheng, H.; Yang, S.; Jiang, W.
Appl. Environ. Microbiol.
71
5290-5296
Morphological, physiological and enzymatic characteristics of cephalosporin acylase-producing Arthrobacter strain 45-8A
1993
Zhang, Q.J.; Xu, W.X.
Arch. Microbiol.
159
392-395
An acidic glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas nitroreducens
1998
Lee, Y.H.; Chang, T.S.; Liu, H.J.; Chu, W.S.
Biotechnol. Appl. Biochem.
28
113-118
Mutational analysis of a key residue in the substrate specificity of a cephalosporin acylase
2004
Otten, L.G.; Sio, C.F.; van der Sloot, A.M.; Cool, R.H.; Quax, W.J.
Chembiochem
5
820-825
High-level production, chemical modification and site-directed mutagenesis of a cephalosporin C acylase from Pseudomonas strain N176
1995
Ishii, Y.; Saito, Y.; Fujimura, T.; Sasaki, H.; Noguchi, Y.; Yamada, H.; Niwa, M.; Shimomura, K.
Eur. J. Biochem.
230
773-778
In vivo post-translational processing and subunit reconstitution of cephalosporin acylase from Pseudomonas sp. 130
1999
Li, Y.; Chen, J.; Jiang, W.; Mao, X.; Zhao, G.; Wang, E.
Eur. J. Biochem.
262
713-719
-
Comparative characterization of new glutaryl 7-ACA and cephalosporin C acylases
1992
Aramori, I.; Fukagawa, M.; Tsumura, M.; Iwami, M.; Ono, H.; Ishitani, Y.; Koji, H.; Kohsaka, M.; Ueda, Y.; Imanaka, H.
J. Ferment. Bioeng.
73
185-192
-
Acetyl esterase from Mediterranean oranges: Partial purification, immobilization and biotransformations
2004
Pasta, P.; Verga, R.; Zambianchi, F.; Daminati, M.
Biocatal. Biotransform.
22
221-224
Heterologous protein production in Acremonium chrysogenum: expression of bacterial cephalosporin C acylase and human thrombomodulin genes
1997
Honda, G.; Matsuda, A.; Zushi, M.; Yamamoto, S.; Komatsu, K.
Biosci. Biotechnol. Biochem.
61
948-955
Crystal structures of complexes between the R61 DD-peptidase and peptidoglycan-mimetic beta-lactams: a non-covalent complex with a "perfect penicillin"
2005
Silvaggi, N.R.; Josephine, H.R.; Kuzin, A.P.; Nagarajan, R.; Pratt, R.F.; Kelly, J.A.
J. Mol. Biol.
345
521-533
Evolution of an acylase active on cephalosporin C
2005
Pollegioni, L.; Lorenzi, S.; Rosini, E.; Marcone, G.L.; Molla, G.; Verga, R.; Cabri, W.; Pilone, M.S.
Protein Sci.
14
3064-3076
Trigonopsis variabilis D-amino aacid oxidase: control of protein quality and opportunities for biocatalysis through production in Escherichia coli
2007
Dib, I.; Stanzer, D.; Nidetzky, B.
Appl. Environ. Microbiol.
73
331-333
Batch production of deacetyl 7-aminocephalosporanic acid by immobilized cephalosporin-C deacetylase
2004
Takimoto, A.; Takakura, T.; Tani, H.; Yagi, S.; Mitsushima, K.
Appl. Microbiol. Biotechnol.
65
263-267
Expression of a cephalosporin C esterase gene in Acremonium chrysogenum for the direct production of deacetylcephalosporin C
2004
Basch, J.; Franceschini, T.; Tonzi, S.; Chiang, S.J.
J. Ind. Microbiol. Biotechnol.
31
531-539
A colorimetric assay for the determination of acetyl xylan esterase or cephalosporin C acetyl esterase activities using 7-amino cephalosporanic acid, cephalosporin C, or acetylated xylan as substrate
2007
Martinez-Martinez, I.; Montoro-Garcia, S.; Lozada-Ramirez, J.D.; Sanchez-Ferrer, A.; Garcia-Carmona, F.
Anal. Biochem.
369
210-217
Characterization and application of D-amino acid oxidase and catalase within permeabilized Pichia pastoris cells in bioconversions
2007
Tan, Q.; Song, Q.; Zhang, Y.; Wei, D.
Appl. Biochem. Biotechnol.
136
279-289
-
Cloning and expression of variants of the glutaryl-7-aminocephalosporic acid acylase of the bacterium Brevundimonas diminuta in Escherichia coli cells
2007
Khatuntseva, S.A.; Eldarov, M.A.; Lopatin, S.A.; Zeinalov, O.A.; Skryabin, K.G.
Appl. Biochem. Microbiol.
43
414-421
Properties and applications of microbial D-amino acid oxidases: current state and perspectives
2008
Pollegioni, L.; Molla, G.; Sacchi, S.; Rosini, E.; Verga, R.; Pilone, M.S.
Appl. Microbiol. Biotechnol.
78
1-16
Stability and stabilization of D-amino acid oxidase from the yeast Trigonopsis variabilis
2007
Nidetzky, B.
Biochem. Soc. Trans.
35
1588-1592
Irreversible inhibition of the Mycobacterium tuberculosis beta-lactamase by clavulanate
2007
Hugonnet, J.E.; Blanchard, J.S.
Biochemistry
46
11998-12004
The role of cofactor binding in tryptophan accessibility and conformational stability of His-tagged D-amino acid oxidase from Trigonopsis variabilis
2007
Arroyo, M.; Menendez, M.; Garcia, J.L.; Campillo, N.; Hormigo, D.; De la Mata, I.; Castillon, M.P.; Acebal, C.
Biochim. Biophys. Acta
1774
556-565
Investigating the role of active site residues of Rhodotorula gracilis D-amino acid oxidase on its substrate specificity
2007
Boselli, A.; Piubelli, L.; Molla, G.; Pilone, M.S.; Pollegioni, L.; Sacchi, S.
Biochimie
89
360-368
-
Optimization of culture condition for the production of D-amino acid oxidase in a recombinant Escherichia coli
2008
Kim, S.; Kim, N.; Shin, C.; Kim, C.
Biotechnol. Bioprocess Eng.
13
144-149
Subunit fusion of two yeast D-amino acid oxidases enhances their thermostability and resistance to H2O2
2008
Wang, S.J.; Yu, C.Y.; Lee, C.K.; Chern, M.K.; Kuan, I.C.
Biotechnol. Lett.
30
1415-1422
Stabilization of native and double D-amino acid oxidases from Rhodosporidium toruloides and Trigonopsis variabilis by immobilization on streptavidin-coated magnetic beads
2008
Wang, S.J.; Yu, C.Y.; Kuan, I.C.
Biotechnol. Lett.
30
1973-1981
Physiological functions of D-amino acid oxidases: from yeast to humans
2007
Pollegioni, L.; Piubelli, L.; Sacchi, S.; Pilone, M.S.; Molla, G.
Cell. Mol. Life Sci.
64
1373-1394
Enzymatic modifications of cephalosporins by cephalosporin acylase and other enzymes
2006
Sonawane, V.C.
Crit. Rev. Biotechnol.
26
95-120
Engineering the properties of D-amino acid oxidases by a rational and a directed evolution approach
2007
Pollegioni, L.; Sacchi, S.; Caldinelli, L.; Boselli, A.; Pilone, M.S.; Piubelli, L.; Molla, G.
Curr. Protein Pept. Sci.
8
600-618
A highly active adipyl-cephalosporin acylase obtained via rational randomization
2007
Otten, L.G.; Sio, C.F.; Reis, C.R.; Koch, G.; Cool, R.H.; Quax, W.J.
FEBS J.
274
5600-5610
Oxystress inducing antitumor therapeutics via tumor-targeted delivery of PEG-conjugated D-amino acid oxidase
2008
Fang, J.; Deng, D.; Nakamura, H.; Akuta, T.; Qin, H.; Iyer, A.K.; Greish, K.; Maeda, H.
Int. J. Cancer
122
1135-1144
Properties of Rhodotorula gracilis D-amino acid oxidase immobilized on magnetic beads through His-tag
2008
Kuan, I.; Liao, R.; Hsieh, H.; Chen, K.; Yu, C.
J. Biosci. Bioeng.
105
110-115
Purification and immobilization of recombinant variants of Brevundimonas diminuta glutaryl-7-aminocephalosporanic acid acylase expressed in Escherichia coli cells
2008
Khatuntseva, S.A.; Eldarov, M.A.; Redo, V.A.; Skryabin, K.G.
J. Biotechnol.
133
123-126
A biosensor for all D-amino acids using evolved D-amino acid oxidase
2008
Rosini, E.; Molla, G.; Rossetti, C.; Pilone, M.S.; Pollegioni, L.; Sacchi, S.
J. Biotechnol.
135
377-384
Characterization and structural modeling of a novel thermostable glycine oxidase from Geobacillus kaustophilus HTA426
2008
Martinez-Martinez, I.; Navarro-Fernandez, J.; Garcia-Carmona, F.; Takami, H.; Sanchez-Ferrer, A.
Proteins
70
1429-1441
-
Media optimization for the production of cephalosporin C acylase from a novel bacterial source: Alcaligenes xylosoxidans MTCC *491
2008
Nupura, H.; Asmita, T.; Sharath, B.; Asmita, P.
Res. J. Biotechnol.
3
16-21
High soluble expression of D-amino acid oxidase in Escherichia coli regulated by a native promoter
2008
Liu, Y.; Li, Q.; Zhu, H.; Yang, J.
Appl. Biochem. Biotechnol.
158
313-322
Characterization and structural modeling of a novel thermostable glycine oxidase from Geobacillus kaustophilus HTA426
2008
Martinez-Martinez, I.; Navarro-Fernandez, J.; Garcia-Carmona, F.; Takami, H.; Sanchez-Ferrer, A.
Proteins Struct. Funct. Bioinform.
70
1429-1441
Crystallization and preliminary diffraction analysis of an engineered cephalosporin acylase
2010
Anandan, A.; Vallet, C.; Coyle, T.; Moustafa, I.M.; Vrielink, A.
Acta Crystallogr. Sect. F
66
808-810
-
Determination of cephalosporin acylase activity by biological and colorimetric method in bacteria
2009
Tanomand, A.; Abeshov, R.; Farajnia, S.
Afr. J. Biotechnol.
8
6697-6699
Biosynthesis of cephalosporin-C acylase enzyme: optimal media design, purification, and characterization
2010
Gaurav, K.; Kundu, K.; Kundu, S.
Artif. Cells Blood Substit. Immobil. Biotechnol.
38
277-283
Enhancement of glutaryl-7-aminocephalosporanic acid acylase activity of gamma-glutamyltranspeptidase of Bacillus subtilis
2010
Suzuki, H.; Yamada, C.; Kijima, K.; Ishihara, S.; Wada, K.; Fukuyama, K.; Kumagai, H.
Biotechnol. J.
5
829-837
A single Phe54Tyr substitution improves the catalytic activity and thermostability of Trigonopsis variabilis D-amino acid oxidase
2010
Wong, K.S.; Fong, W.P.; Tsang, P.W.
New Biotechnol.
27
78-84
Characteristic of immobilized cephalosporin C acylase and its application in one-step enzymatic conversion of cephalosporin C to 7-aminocephalosporanic acid
2011
Zhu, X.; Luo, H.; Chang, Y.; Su, H.; Li, Q.; Yu, H.; Shen, Z.
World J. Microbiol. Biotechnol.
27
823-829
Hyperthermostable acetyl xylan esterase
2010
Drzewiecki, K.; Angelov, A.; Ballschmiter, M.; Tiefenbach, K.J.; Sterner, R.; Liebl, W.
Microb. Biotechnol.
3
84-92
Purification and characterization of YxeI, a penicillin acylase from Bacillus subtilis
2012
Rathinaswamy, P.; Gaikwad, S.M.; Suresh, C.G.; Prabhune, A.A.; Brannigan, J.A.; Dodson, G.G.; Pundle, A.V.
Int. J. Biol. Macromol.
50
25-30
The N-terminal nucleophile serine of cephalosporin acylase executes the second autoproteolytic cleavage and acylpeptide hydrolysis
2011
Yin, J.; Deng, Z.; Zhao, G.; Huang, X.
J. Biol. Chem.
286
24476-24486
Overexpression of synthesized cephalosporin C acylase containing mutations in the substrate transport tunnel
2012
Wang, Y.; Yu, H.; Song, W.; An, M.; Zhang, J.; Luo, H.; Shen, Z.
J. Biosci. Bioeng.
113
36-41
-
On the substrate preference of glutaryl acylases
2012
Rosini, E.; Monelli, C.; Pollegioni, L.; Riva, S.; Monti, D.
J. Mol. Catal. B
76
52-58
New biotech applications from evolved D-amino acid oxidases
2011
Pollegioni, L.; Molla, G.
Trends Biotechnol.
29
276-283
Double knockout of beta-lactamase and cephalosporin acetyl esterase genes from Escherichia coli reduces cephalosporin C decomposition
2012
Wang, Y.; Yu, H.; Zhang, J.; Luo, H.; Shen, Z.
J. Biosci. Bioeng.
113
737-741
Functional and structural characterization of a thermostable acetyl esterase from Thermotoga maritima
2012
Levisson, M.; Han, G.W.; Deller, M.C.; Xu, Q.; Biely, P.; Hendriks, S.; Ten Eyck, L.F.; Flensburg, C.; Roversi, P.; Miller, M.D.; McMullan, D.; von Delft, F.; Kreusch, A.; Deacon, A.M.; van der Oost, J.; Lesley, S.A.; Elsliger, M.A.; Kengen, S.W.; Wilson, I.A.
Proteins
80
1545-1559
Structure of a class III engineered cephalosporin acylase: Comparisons with class I acylase and implications for differences in substrate specificity and catalytic activity
2013
Golden, E.; Paterson, R.; Tie, W.; Anandan, A.; Flematti, G.; Molla, G.; Rosini, E.; Pollegioni, L.; Vrielink, A.
Biochem. J.
451
217-226
Strategic manipulation of an industrial biocatalyst - evolution of a cephalosporin C acylase
2014
Conti, G.; Pollegioni, L.; Molla, G.; Rosini, E.
FEBS J.
281
2443-2455
Determination of the second autoproteolytic cleavage site of cephalosporin C acylase and the effect of deleting its flanking residues in the alpha-C-terminal region
2014
Zhang, J.; Yu, H.; Wang, Y.; Luo, H.; Shen, Z.
J. Biotechnol.
184
138-145
Characterization of cross-linked immobilized arylesterase from Gluconobacter oxydans 621H with activity toward cephalosporin C and 7-aminocephalosporanic acid
2016
Inmaculada, N.; Francisco, G.
Biotechnol. Prog.
32
36-42
A high-throughput enzyme-coupled assay for SAMHD1 dNTPase
2015
Seamon, K.J.; Stivers, J.T.
J. Biomol. Screen.
20
801-809
Purification and partial characterization of novel penicillin V acylase from Acinetobacter sp. AP24 isolated from Loktak Lake, an Indo-Burma biodiversity hotspot
2016
Philem, P.D.; Sonalkar, V.V.; Dharne, M.S.; Prabhune, A.A.
Prep. Biochem. Biotechnol.
46
524-530
Computational design of variants for cephalosporin C acylase from Pseudomonas strain N176 with improved stability and activity
2017
Tian, Y.; Huang, X.; Li, Q.; Zhu, Y.
Appl. Microbiol. Biotechnol.
101
621-632
-
Cephalosporin C acylase from Pseudomonas species production and enhancement of its activity by optimization of process parameters
2015
Jobanputra, A.; Vasait, R.
Biocatal. Agricult. Biotechnol.
4
465-470
-
Reversible immobilization of cephalosporin C acylase on epoxy supports coated with polyethyleneimine
2015
Wei, Y.; Luo, H.; Chang, Y.; Yu, H.; Shen, Z.
Biocatal. Biotransform.
33
250-259
Microenvironmental pH changes in immobilized cephalosporin C acylase during a proton-producing reaction and regulation by a two-stage catalytic process
2017
Luo, H.; Zhu, L.; Chang, Y.; Liu, X.; Liu, Z.; Sun, H.; Li, X.; Yu, H.; Shen, Z.
Biores. Technol.
223
157-165
-
An effective method for extraction of glutaryl-7-aminocephalosporanic acid acylase from recombinant E. coli cells
2015
Ma, X.; Su, E.; Deng, S.; Wei, D.
Biotechnol. Bioprocess Eng.
20
718-724
-
One-pot conversion of cephalosporin C by using an optimized two-enzyme process
2015
Conti, G.; Pollegioni, L.; Rosini, E.
Catal. Sci. Technol.
5
1854-1863
-
Effects of inhibitors on the catalysis and immobilization of cephalosporin C acylase
2018
Luo, H.; Han, K.; Chang, Y.; Wang, Y.; Tong, S.; Nie, Z.; Wang, X.; Wei, Y.; Yu, H.; Shen, Z.
Chem. Biochem. Eng. Q.
32
125-133
-
Computational design of thermostable mutants for cephalosporin C acylase from Pseudomonas strain SE83
2018
Xu, Z.; Tian, Y.; Zhu, Y.
Comput. Chem. Eng.
116
112-121
Modelling of substrate access and substrate binding to cephalosporin acylases
2019
Ferrario, V.; Fischer, M.; Zhu, Y.; Pleiss, J.
Sci. Rep.
9
12402
A novel thermostable D-amino acid oxidase of the thermophilic fungus Rasamsonia emersonii strain YA
2019
Shimekake, Y.; Furuichi, T.; Abe, K.; Kera, Y.; Takahashi, S.
Sci. Rep.
9
11948
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