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Ligand uracil-mismatched double-stranded DNA with U-G mismatch Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Basic Ligand Information
uracil-mismatched double-stranded DNA with U-G mismatch
Roles as Enzyme Ligand
Substrate in Enzyme-catalyzed Reactions (1 result)
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uracil-mismatched double-stranded DNA with U-G mismatch + H2O = uracil + double-stranded DNA with abasic site
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Enzyme Kinetic Parameters
kcat Value (Turnover Number) (4 results)
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0.014
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pH 7.5, 37°C, wild-type enzyme
0.055
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pH 7.5, 30°C, recombinant hSMUG1
5.57
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pH 7.5, 30°C, recombinant hUNG2
KM Value (4 results)
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0.0000022
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pH 7.5, 37°C, wild-type enzyme
0.0004
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pH 7.5, 30°C, recombinant hUNG2
0.0013
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pH 7.5, 30°C, recombinant hSMUG1
References & Links Literature References (3)
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Stukenberg, P.T.; Kirschner, M.W.; Verdine, G.L.: Identification of a new uracil-DNA glycosylase family by expression cloning using synthetic inhibitors
1999
Haushalter, K.A
Curr. Biol.
9
174-185
hUNG2 is the major repair enzyme for removal of uracil from U:A matches, U:G mismatches, and U in single-stranded DNA, with hSMUG1 as a broad specificity backup
2002
Kavli, B.; Sundheim, O.; Akbari, M.; Otterlei, M.; Nilsen, H.; Skorpen, F.; Aas, P.A.; Hagen, L.; Krokan, H.E.; Slupphaug, G.
J. Biol. Chem.
277
39926-39936
Mutational analysis of the damage-recognition and catalytic mechanism of human SMUG1 DNA glycosylase
2004
Matsubara, M.; Tanaka, T.; Terato, H.; Ohmae, E.; Izumi, S.; Katayanagi, K.; Ide, H.
Nucleic Acids Res.
32
5291-5302
Links to other databases for uracil-mismatched double-stranded DNA with U-G mismatch
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