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Basic Ligand Information
Roles as Enzyme Ligand
Enzyme Kinetic Parameters
References & Links
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Ligand 5,6,7,8-tetrahydropteroyl-Glu2

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Basic Ligand Information

Molecular Structure

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Molecular Formula

BRENDA Name

InChIKey

C₂₄H₃₀N₈O₉

5,6,7,8-tetrahydropteroyl-Glu2

ZAOGJXDWOQXFBW-KKUMJFAQSA-N

Synonyms:

(6S)-5,6,7,8-tetrahydrofolyl-Glu, (6S)-5,6,7,8-tetrahydropteroyl-Glu2, 5,6,7,8-tetrahydrofolyl-Glu, 5,6,7,8-tetrahydropteroyl-gamma-Glu2, tetrahydropteroyl-Glu2, tetrahydropteroyldiglutamate, THF-L-glutamate

Pathway Source

Pathways

KEGG

Biosynthesis of cofactors, Folate biosynthesis

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (1 result)

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EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.1.2.11

tetrahydropteroyldiglutamate + 2-dehydropantoate = 5,10-methylenetetrahydropteroyldiglutamate + 3-methyl-2-oxobutanoate + H2O

485700

Product in Enzyme-catalyzed Reactions (12 results)

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EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

6.3.2.17

ATP + (6RS)-5,6,7,8-tetrahydrofolic acid + L-glutamate = ADP + phosphate + (6S)-5,6,7,8-tetrahydrofolyl-Glu

0, 650201

6.3.2.17

ATP + (6S)-5,6,7,8-tetrahydropteroylglutamate + L-glutamate = ADP + phosphate + (6S)-5,6,7,8-tetrahydropteroyl-Glu2

0, 650131

6.3.2.17

ATP + 5,6,7,8-tetrahydrofolate + L-glutamate = ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu

6.3.2.17

ATP + 5,6,7,8-tetrahydrofolic acid + L-Glu = ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu

6.3.2.17

ATP + 5,6,7,8-tetrahydrofolic acid + L-glutamate = ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu

653665, 652828, 652860

6.3.2.17

ATP + 5,6,7,8-tetrahydropteroyl-gamma-Glu + Glu = ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu2

6.3.2.17

ATP + 5,6,7,8-tetrahydropteroyl-Glu + L-glutamate = ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu2

6.3.2.17

ATP + 5,6,7,8-tetrahydropteroyl-Glu + L-glutamate = ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2

6.3.2.17

CTP + 5,6,7,8-tetrahydropteroyl-Glu + Glu = CDP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2

6.3.2.17

dATP + 5,6,7,8-tetrahydropteroyl-Glu + Glu = dADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2

6.3.2.17

ITP + 5,6,7,8-tetrahydropteroyl-Glu + Glu = IDP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2

6.3.2.17

UTP + 5,6,7,8-tetrahydropteroyl-Glu + Glu = UDP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2

6.3.2.17

12 entries

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (1 result)

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EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

6.3.2.17

0.81

1089

K_M Value (3 results)

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EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

0.25

0.0033

0.0034

1060, 1061, 1063

6.3.2.17

2 entries

References & Links

Literature References (10)

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BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

1060

3828322

Mammalian folylpoly-gamma-glutamate synthetase. 3. Specificity of folate analogues

1987

George, S.; Cichowicz, D.J.; Shane, B.

Biochemistry

522-529

1061

3828321

Mammalian folylpoly-gamma-glutamate synthetase. 2. Substrate specificity and kinetic properties

1987

Cichowicz, D.J.; Shane, B.

Biochemistry

513-521

1063

Folylpoly-gamma-glutamate synthetase

1986

Shane, B.

Chem. Biol. Pteridines, Proc. Int. Symp. Pteridines Folic Acid Deriv. Chem. Biol. Clin. Aspects

719-728

1089

9250977

Human cytosolic folylpoly-gamma-glutamate synthase

1997

Atkinson, I.; Garrow, T.; Brenner, A.; Shane, B.

Methods Enzymol.

281

134-140

485700

6463

Ketopantoate hydroxymethyltransferase. II. Physical, catalytic, and regulatory properties

1976

Powers, S.G.; Snell, E.E.

J. Biol. Chem.

251

3786-3793

650131

11772020

Mouse folylpoly-gamma-glutamate synthetase isoforms respond differently to feedback inhibition by folylpolyglutamate cofactors

2002

Andreassi, J.L.2nd.; Moran, R.G.

Biochemistry

226-235

650201

12578366

Binding of ATP as well as tetrahydrofolate induces conformational changes in Lactobacillus casei folylpolyglutamate synthetase in solution

2003

Sheng, Y.; Ip, H.; Liu, J.; Davidson, A.; Bognar, A.L.

Biochemistry

1537-1543

652828

10970743

Structural and functional similarities in the ADP-forming amide bond ligase superfamily: implications for a substrate-induced conformational change in folylpolyglutamate synthetase

2000

Sheng, Y.; Sun, X.; Shen, Y.; Bognar, A.L.; Baker, E.N.; Smith, C.A.

J. Mol. Biol.

302

427-440

652860

11501996

Folate-binding triggers the activation of folylpolyglutamate synthetase

2001

Sun, X.; Cross, J.A.; Bognar, A.L.; Baker, E.N.; Smith, C.A.

J. Mol. Biol.

310

1067-1078

653665

11752472

Tetrahydrofolate biosynthesis in plants: molecular and functional characterization of dihydrofolate synthetase and three isoforms of folylpolyglutamate synthetase in Arabidopsis thaliana

2001

Ravanel, S.; Cherest, H.; Jabrin, S.; Grunwald, D.; Surdin-Kerjan, Y.; Douce, R.; Rebeille, F.

Proc. Natl. Acad. Sci. USA

15360-15365