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Ligand globotriosylceramide Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Basic Ligand Information Molecular Structure
C5 8 H1 0 9 NO1 8
globotriosylceramide
IPRFVQMZNCETMH-TWFDOCQMSA-N
beta-Gal-(1->4)-beta-Gal-(1->4)-beta-Glc-(1<->1)-Cer (4E-d18:1;C22:0), ganglioside Gb3-Cer, ganglioside Gb3-ceramide, ganglioside Gb3Cer, Gb3-liposomes, globotriglycosylceramide
Roles as Enzyme Ligand
In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)
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UDP-N-acetyl-D-galactosamine + globotriosylceramide = UDP + globotetraosylceramide
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Substrate in Enzyme-catalyzed Reactions (3 results)
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UDP-N-acetyl-D-galactosamine + globotriosylceramide = UDP + globotetraosylceramide
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ganglioside Gb3Cer + H2O = ?
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ganglioside Gb3-ceramide + H2O = ?
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Inhibitor in Enzyme-catalyzed Reactions (1 result)
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inhibition of cell toxicity
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Enzyme Kinetic Parameters
kcat Value (Turnover Number) (2 results)
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0.069
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isoform EHCase II, pH 5.5 and 37°C
85
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isoform EHCase I, pH 5.5 and 37°C
KM Value (3 results)
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0.23
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isoform EHCase II, pH 5.5 and 37°C
0.46
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isoform EHCase I, pH 5.5 and 37°C
References & Links Literature References (4)
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A novel glycosphingolipid hydrolyzing enzyme, glycosphingolipid ceramide deacylase, which cleaves the linkage between the fatty acid and sphingosine base in glycosphingolipids
1988
Hirabayashi, Y.; Kimura, M.; Matsumoto, M.; Yamamoto, K.; Kadowaki, S.; Tochikura, T.
J. Biochem.
103
1-4
Characterization of a glycosphingolipid beta-N-acetylgalactosaminyl-transferase activity in cultured hamster (nil) cells
1982
Lockney, M.W.; Sweely, C.S.
Biochim. Biophys. Acta
712
234-241
Induction of cytokines by toxins that have an identical RNA N-glycosidase activity: Shiga toxin, ricin, and modeccin
2004
Yamasaki, C.; Nishikawa, K.; Zeng, X.T.; Katayama, Y.; Natori, Y.; Komatsu, N.; Oda, T.
Biochim. Biophys. Acta
1671
44-50
Preparation and characterization of EGCase I, applicable to the comprehensive analysis of GSLs, using a rhodococcal expression system
2012
Ishibashi, Y.; Kobayashi, U.; Hijikata, A.; Sakaguchi, K.; Goda, H.M.; Tamura, T.; Okino, N.; Ito, M.
J. Lipid Res.
53
2242-2251
Links to other databases for globotriosylceramide