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Ligand Gly-Gly-Gly

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

In Vivo Product in Enzyme-catalyzed Reactions (1 result)

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Show Natural Product (1 entry)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.4.24.75

pentaglycine + H2O = diglycine + triglycine

0

-

Substrate in Enzyme-catalyzed Reactions (11 results)

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Show Substrate (11 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.3.2.2

5-L-glutamyl-4-nitroanilide + Gly-Gly-Gly = 4-nitroaniline + 5-L-glutamyl-Gly-Gly-Gly

676991

-

3.4.11.19

Gly-Gly-Gly + 2 H2O = 3 Gly

752577

-

3.4.11.4

Gly-Gly-Gly + H2O = Gly + Gly-Gly

35931, 35927

-

3.4.14.1

Gly-Gly-Gly + H2O = Gly-Gly + Gly

36146

-

3.4.16.4

S2a + Gly-Gly-Gly = ?

670827

-

3.4.22.40

Gly-Gly-Gly + H2O = ?

36712

-

3.4.22.70

aminobenzoyl-LPETG-dinitrophenyl ester + Gly-Gly-Gly = aminobenzoyl-LPETGGG + Gly-dinitrophenyl ester

677526

-

3.4.22.70

o-aminobenzoyl-Leu-Pro-Glu-Thr-Gly-2,4-dinitrophenyl ester + Gly-Gly-Gly = o-aminobenzoyl-Leu-Pro-Glu-Thr-Gly-Gly-Gly + Gly-2,4-dinitrophenyl ester

647379

-

3.4.22.70

AHLPKTGLR-NH2 + triglycine = ?

707524

-

3.4.22.70

recombinant Helicobacter pylori R1,3-fucosyltransferase + triglycine = ?

707524

-

3.4.22.70

4 entries

3.5.1.32

glycylglycylglycine + H2O = glycine + glycylglycine

172035

-

Product in Enzyme-catalyzed Reactions (5 results)

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Show Product (5 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.4.11.5

Pro-Gly-Gly-Gly + H2O = Pro + Gly-Gly-Gly

0

-

3.4.24.75

pentaglycine + H2O = diglycine + triglycine

0

-

3.5.1.32

N-(4-nitrobenzoyl)-Gly-Gly-Gly + H2O = 4-nitrobenzoic acid + Gly-Gly-Gly

172033

-

3.5.1.32

N-benzoylglycylglycylglycine + H2O = benzoic acid + glycylglycylglycine

172033

-

3.5.1.32

2 entries

7.4.2.9

ATP + H2O + Gly-Gly-Gly-[tripeptide-binding protein][side 1] = ADP + phosphate + Gly-Gly-Gly[side 2] + [tripeptide-binding protein][side 1]

0

-

Inhibitor in Enzyme-catalyzed Reactions (1 result)

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Show Inhibitors (1 entry)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

2.3.1.13

about 37% inhibition at 100 mM

737197

-

3D Structure of Enzyme-Ligand-Complex (PDB) (3 results)

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Show 3D Structure of Enzyme-Ligand-Complex (PDB) (3 entries)

EC NUMBER

ENZYME 3D STRUCTURE

1.1.1.87

3ty3, 3D-view

3.4.21.64

6kkf, 3D-view

3.5.1.98

5thu, 3D-view

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (2 results)

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Show Turnover Number (2 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

3.4.11.4

2030

-

-

35931

3.4.22.70

0.18

-

-

677526

K_M Value (5 results)

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Show KM Value (5 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

3.4.11.4

7.5

-

-

35927

3.4.11.4

20

-

-

35931

3.4.11.4

2 entries

3.4.22.40

1.05

-

-

36712

3.4.22.70

0.041

-

pH 7.5, transpeptidation with o-aminobenzoyl-Leu-Pro-Glu-Thr-Gly-2,4-dinitrophenol

647379

3.4.22.70

0.2

-

-

677526

3.4.22.70

2 entries

References & Links

Literature References (13)

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Show Reference (13 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

35927

109082

Aminotripeptidase, a cytosol enzyme from rabbit intestinal mucosa

1979

Doumeng, C.; Maroux, S.

Biochem. J.

177

801-808

35931

4694732

Aminotripeptidase of swine kidney. I. Isolation and characterization of three different forms; utility of the enzyme in sequence work

1973

Chenoweth, D.; Mitchel, R.E.J.; Smith, E.L.

J. Biol. Chem.

248

1672-1683

36146

4306035

New observations on the substrate specificity of cathepsin C (dipeptidyl aminopeptidase I). Including the degradation of beta-corticotropin and other peptide hormones

1969

McDonald, J.K.; Zeitman, B.B.; Reilly, T.J.; Ellis, S.

J. Biol. Chem.

244

2693-2709

36712

9546047

Catalytic properties of the cysteine aminopeptidase PepC, a bacterial bleomycin hydrolase

1998

Mistou, M.Y.; Gripon, J.C.

Biochim. Biophys. Acta

1383

63-70

172033

-

Crystallization and properties of N-benzoylglycine amidohydrolase from Pseudomonas putida

1985

Miyagawa, E.; Yano, J.; Hamakado, T.; Kido, Y.; Nishimoto, K.; Motoki, Y.

Agric. Biol. Chem.

49

2881-2886

172035

-

Hippurathydrolase, ein neues Enzym aus Mikroorganismen, Darstellung einer gereinigten Enzympräparation und Untersuchungen zur Substratspezifität

1968

Röhr, M.

Monatsh. Chem.

99

2255-2277

647379

14503881

Kinetic mechanism of Staphylococcus aureus sortase SrtA

2003

Huang, X.; Aulabaugh, A.; Ding, W.; Kapoor, B.; Alksne, L.; Tabei, K.; Ellestad, G.

Biochemistry

42

11307-11315

670827

16199665

Specificity and reversibility of the transpeptidation reaction catalyzed by the Streptomyces R61 D-Ala-D-Ala peptidase

2005

Rhazi, N.; Delmarcelle, M.; Sauvage, E.; Jacquemotte, F.; Devriendt, K.; Tallon, V.; Ghosez, L.; Frere, J.M.

Protein Sci.

14

2922-2928

676991

17043758

Identification and characterization of a gamma-glutamyl transpeptidase from a thermo-alcalophile strain of Bacillus pumilus

2006

Moallic, C.; Dabonne, S.; Colas, B.; Sine, J.P.

Protein J.

25

391-397

677526

17107653

Development of an HPLC assay for Staphylococcus aureus sortase: evidence for the formation of the kinetically competent acyl enzyme intermediate

2007

Aulabaugh, A.; Ding, W.; Kapoor, B.; Tabei, K.; Alksne, L.; Dushin, R.; Zatz, T.; Ellestad, G.; Huang, X.

Anal. Biochem.

360

14-22

707524

20178374

Highly oriented recombinant glycosyltransferases: site-specific immobilization of unstable membrane proteins by using Staphylococcus aureus sortase A

2010

Ito, T.; Sadamoto, R.; Naruchi, K.; Togame, H.; Takemoto, H.; Kondo, H.; Nishimura, S.

Biochemistry

49

2604-2614

737197

24576660

Expression, purification, and characterization of mouse glycine N-acyltransferase in Escherichia coli

2014

Dempsey, D.R.; Bond, J.D.; Carpenter, A.M.; Rodriguez Ospina, S.; Merkler, D.J.

Protein Expr. Purif.

97

23-28

752577

22005737

Enzymatic properties of the glycine D-alanine aminopeptidase of Aspergillus oryzae and its activity profiles in liquid-cultured mycelia and solid-state rice culture (rice koji)

2012

Marui, J.; Matsushita-Morita, M.; Tada, S.; Hattori, R.; Suzuki, S.; Amano, H.; Ishida, H.; Yamagata, Y.; Takeuchi, M.; Kusumoto, K.

Appl. Microbiol. Biotechnol.

93

655-669

Links to other databases for Gly-Gly-Gly

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Show Links (1 entry)

ChEBI

PubChem