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Ligand (4R)-4-hydroxy-2-oxopentanoate

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (2 results)

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Show Substrate (2 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

4.1.3.39

(4R)-4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate

714244

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4.1.3.43

(4R)-4-hydroxy-2-oxopentanoate = pyruvate + acetaldehyde

714244, 722458

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Product in Enzyme-catalyzed Reactions (1 result)

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Show Product (1 entry)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

4.1.3.43

pyruvate + acetaldehyde = (4R)-4-hydroxy-2-oxopentanoate

0

-

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (6 results)

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Show Turnover Number (6 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

4.1.3.39

0.131

-

Y290F mutant protein, pH 8.0, 25°C

714244

4.1.3.39

0.17

-

Y290S mutant protein, pH 8.0, 25°C

714244

4.1.3.43

0.043

-

L87W/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C

722458

4.1.3.43

0.098

-

L87N/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C

722458

4.1.3.43

0.131

-

Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C

714244, 722458

4.1.3.43

0.17

-

Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C

714244

4.1.3.39

2 entries

4.1.3.43

4 entries

K_M Value (7 results)

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Show KM Value (7 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

4.1.3.39

0.012

-

Y290S mutant protein, pH 8.0, 25°C

714244

4.1.3.39

0.013

-

Y290F mutant protein, pH 8.0, 25°C

714244

4.1.3.43

0.012

-

Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C

714244

4.1.3.43

0.013

-

Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C

722458

4.1.3.43

0.013

-

Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C

714244

4.1.3.43

0.435

-

L87W/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C

722458

4.1.3.43

0.757

-

L87N/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C

722458

4.1.3.39

2 entries

4.1.3.43

5 entries

References & Links

Literature References (2)

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Show Reference (2 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

714244

21425833

Probing the molecular basis of substrate specificity, stereospecificity, and catalysis in the class II pyruvate aldolase, BphI

2011

Baker, P.; Carere, J.; Seah, S.Y.

Biochemistry

50

3559-3569

722458

22081904

Rational design of stereoselectivity in the class II pyruvate aldolase BphI

2012

Baker, P.; Seah, S.Y.K.

J. Am. Chem. Soc.

134

507-513

Links to other databases for (4R)-4-hydroxy-2-oxopentanoate

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Show Links (1 entry)

ChEBI

PubChem

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