Ligand GDP-fucose

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Basic Ligand Information

Molecular Structure
Picture of GDP-fucose (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C16H25N5O15P2
GDP-fucose
LQEBEXMHBLQMDB-IXDFUAJQSA-N

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (4 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
GDP-fucose + TSR4 = GDP + fucosyl-TSR4
-
show the reaction diagram
GDP-fucose + Gal-beta-1,4GlcNAc-beta-1-R = GDP + Gal-beta-1,4(fuc-alpha-1,3)GlcNAc-beta-1-R
-

Substrate in Enzyme-catalyzed Reactions (112 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE

Inhibitor in Enzyme-catalyzed Reactions (4 results)

COMMENTARY
EC NUMBER
LITERATURE
ENZYME 3D STRUCTURE
competitive with respect to NADPH and GDP-4-keto-6-deoxymannose
-
competitive inhibition

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (1 result)

COMMENTARY
EC NUMBER
LITERATURE
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
pH and temperature not specified in the publication
2.4
-

KM Value (42 results)

COMMENTARY
EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
LITERATURE
pH 7.2, 37°C
0.0065
-
pH 6.5, 37°C
0.0016
-
-
0.043
-
LacNAcbeta-O-(CH2)5CO2CH3 as substrate
0.025
-
LacNAc as substrate
0.06
-
-
0.065
-
FucT 45, Y246F mutant
0.157
-
FucT 45, Y246A mutant
0.8
-
FucT 45, R195K mutant
0.59
1
FucT 45, N240A mutant
0.08016
-
FucT 45
0.08831
-
deletion mutant FTdelta80 with 80 residues deleted from the C-terminus
0.09864
-
deletion mutant FTdelta66 with 66 residues deleted from the C-terminus
0.07864
-
deletion mutant FTdelta45 with 45 residues deleted from the C-terminus
0.08831
-
deletion mutant FTdelta38 with 38 residues deleted from the C-terminus
0.07967
-
deletion mutant FTdelta28 with 28 residues deleted from the C-terminus
0.09356
-
deletion mutant FTdelta23 with 23 residues deleted from the C-terminus
0.0683
-
deletion mutant FTdelta115 with 115 residues deleted from the C-terminus
0.228
-
FucT 115
0.2223
-
-
0.11
-
pH and temperature not specified in the publication
0.0098
-
with beta-Gal1,4-GlcNAc-O-(CH2)8CO2CH3, chimeric FucT 11639(1-359)UA948(361-462)
0.153
-
with Gal-beta-1,4beta-GlcNAc-O-(CH2)8CO2CH3, wild type, strain NCTC116639
0.048
-
with Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3, chimeric FucT 11639(347CNDAHYSALH)
0.0546
-
with Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3, chimeric FucT UA948(1-360)11639(360-478)
0.188
-
with Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3, chimeric FucT UA948(345DNPFIFC)
0.213
-
with Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3, strain UA948
0.0357
-
with Gal-beta-1,4-GlcNAc-O-(CH2)8CO2CH3, chimeric FucT 11639(347CNDAHYSALH)
0.244
-
with Gal-beta-1,4-GlcNAc-O-(CH2)8CO2CH3, chimeric FucT UA948(1-360)11639(360-478)
0.236
-
with Gal-beta-1,4-GlcNAc-O-(CH2)8CO2CH3, chimeric FucT UA948(345DNPFIFC)
0.0481
-
with Gal-beta-1,4-GlcNAc-O-(CH2)8CO2CH3, strain UA948
0.0683
-
wildtype, with Fuc-alpha-1,2Gal-beta-1,4GlcNAc-sp-biotin
0.038
-
wildtype, with Fuc-alpha-1,2Gal-beta-1,3GlcNAc-sp-biotin
0.035
-
mutant W124Y, with Fuc-alpha-1,2Gal-beta-1,4GlcNAc-sp-biotin
0.035
-
mutant W124R, with Fuc-alpha-1,2Gal-beta-1,4GlcNAc-sp-biotin
0.03
-
mutant W111Y, with Fuc-alpha-1,2Gal-beta-1,3GlcNAc-sp-biotin
0.034
-
mutant W111F, with Fuc-alpha-1,2Gal-beta-1,3GlcNAc-sp-biotin
0.037
-
mutant W111A, with Fuc-alpha-1,2Gal-beta-1,3GlcNAc-sp-biotin
0.046
-
-
0.0504
-
pH 6.0, 37°Cmutant enzyme F280SS
0.0909
-
pH 6.0, 37°C. wild-type enzyme
0.05
-
pH 6.0, 37°C, mutant enzyme G247S
0.178
-

Ki Value (4 results)

COMMENTARY
EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
LITERATURE
-
0.055
-
LacNAc as substrate
0.0062
-
LacNAcbeta-O-(CH2)5CO2CH3 as substrate
0.031
-
pH 7, 37°C
0.0789
-

References & Links

Links to other databases for GDP-fucose

ChEBI
PubChem
-
PubChem