Ligand 25-hydroxyvitamin D3

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Basic Ligand Information

Molecular Structure
Picture of 25-hydroxyvitamin D3 (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C27H44O2
25-hydroxyvitamin D3
JWUBBDSIWDLEOM-DTOXIADCSA-N
Synonyms:
25-hydroxy-vitamin D3, 25-hydroxy-[3alpha-3H]vitamin D3, 25-hydroxy-[26,27-3H]vitamin D3, 25-hydroxyvitamin D, calcidiol


Show all pahtways known for Show all BRENDA pathways known for 25-hydroxyvitamin D3

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (8 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE

In Vivo Product in Enzyme-catalyzed Reactions (4 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
calciol + O2 + [reduced NADPH-hemoprotein reductase] = calcidiol + [oxidized NADPH-hemoprotein reductase] + H2O
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vitamin D3 + acceptor + H2O = 25-hydroxy-vitamin D3 + reduced acceptor
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Substrate in Enzyme-catalyzed Reactions (17 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
25-hydroxyvitamin D3 + O2 + NADPH + H+ = 1alpha,25-dihydroxyitamin D3 + NADP+ + H2O
show the reaction diagram
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Product in Enzyme-catalyzed Reactions (8 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
vitamin D3 + acceptor + H2O = 25-hydroxy-vitamin D3 + reduced acceptor
show the reaction diagram
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Inhibitor in Enzyme-catalyzed Reactions (2 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
CYP27B1 shows inhibition when substrate concentrations in the membrane are greater than 4 times Km, more pronounced with 25-hydroxyvitamin D3 than with 25-hydroxyvitamin D2
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Enzyme Kinetic Parameters

kcat Value (Turnover Number) (9 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.002
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in 20 mM Tris (pH 7.5), at 37°C
0.0023
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1alpha-hydroxylation, mutant R73V/R84A, pH not specified in the publication, temperature not specified in the publication

KM Value (12 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.0071
-
30°C, pH not specified in the publication

References & Links

Links to other databases for 25-hydroxyvitamin D3

ChEBI
PubChem
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PubChem