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Ligand raffinose

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (10 results)

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Show Natural Substrate (10 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.3.9

2 raffinose + 2 O2 = 6''-aldehydoraffinose + 6''-carboxyraffinose + H2O2 + H2O

389866, 389863

-

1.1.3.9

raffinose + O2 = ? + H2O2

389862, 389881, 389864, 389882, 389851

-

2.4.1.166

raffinose + raffinose = isolychnose + sucrose

488685

-

2.4.1.166

raffinose + raffinose = lychnose + sucrose

488685

-

2.4.1.67

1-alpha-D-galactosyl-myo-inositol + raffinose = myo-inositol + stachyose

638260, 638266, 638265, 723481, 638262, 638263, 638264

-

2.4.1.67

alpha-D-galactosyl-(1->3)-1D-myo-inositol + raffinose = myo-inositol + stachyose

737276, 736162, 736151

-

3.2.1.22

raffinose + H2O = D-fructose + ?

750215

-

3.2.1.22

raffinose + H2O = sucrose + alpha-D-galactose

715877, 655041

-

3.2.1.22

raffinose + H2O = sucrose + D-galactose

749435, 750624, 749778

-

1.1.3.9

2 entries

2.4.1.166

2 entries

2.4.1.67

2 entries

3.2.1.22

3 entries

3.2.1.26

raffinose + H2O = D-fructose + melibiose

665943

-

In Vivo Product in Enzyme-catalyzed Reactions (2 results)

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Show Natural Product (2 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.82

1-alpha-D-galactosyl-myo-inositol + sucrose = myo-inositol + raffinose

0

-

2.4.1.82

alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose

0

-

2.4.1.82

2 entries

Substrate in Enzyme-catalyzed Reactions (70 results)

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Show Substrate (70 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.14

D-raffinose + NADH = ?

285900

-

1.1.3.9

2 raffinose + 2 O2 = 6''-aldehydoraffinose + 6''-carboxyraffinose + H2O2 + H2O

389866, 389863

-

1.1.3.9

D-raffinose + O2 = ? + H2O2

763465

-

1.1.3.9

raffinose + O2 = 6''-aldehydoraffinose + 6''-carboxyraffinose + H2O2 + H2O

743101, 743337, 743734

-

1.1.3.9

raffinose + O2 = ?

672854, 687434

-

1.1.3.9

raffinose + O2 = ? + H2O2

389862, 389881, 389864, 389882, 389851

-

1.1.3.9

5 entries

1.1.5.9

D-raffinose + 2,6-dichlorophenolindophenol = ? + reduced 2,6-dichlorophenolindophenol

740363

-

1.1.99.13

raffinose + acceptor = ?

389892, 389893

-

1.1.99.29

raffinose + ferrocenium ion = ?

741362

-

2.4.1.10

D-raffinose = D-fructose + melibiose

685846

-

2.4.1.10

raffinose + (2,6-beta-D-fructosyl)n = galactose + (2,6-beta-D-fructosyl)n+1

488343, 488316, 488324

-

2.4.1.10

sucrose + raffinose = Fru(beta-2,1)Gal + fructosylated lactose + fructosylated cellobiose + 1-kestotriose + 6-kestotriose + ?

755877

-

2.4.1.10

sucrose + raffinose = melibiose + ?

684765

-

2.4.1.10

sucrose + raffinose = melibiose + D-fructose + levan

756953

-

2.4.1.10

5 entries

2.4.1.140

sucrose + raffinose = ?

703062

-

2.4.1.162

alpha-D-galactopyranosyl-(1->6)-O-alpha-D-glucopyranosyl-(1->2)-D-fructofuranoside + sucrose = alpha-D-galactopyranosyl-(1->6)-O-alpha-D-glucopyranoside + ?

488677

-

2.4.1.162

raffinose + D-xylose = beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside + alpha-D-galactopyranosyl alpha-D-glucopyranoside

692116

-

2.4.1.162

raffinose + galactose = beta-D-fructofuranosyl-2,1-D-galactopyranoside + alpha-D-galactopyranosyl alpha-D-glucopyranoside

488677

-

2.4.1.162

raffinose + xylose = beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside + alpha-D-galactopyranosyl alpha-D-glucopyranoside

488677

-

2.4.1.166

raffinose + raffinose = isolychnose + sucrose

488685

-

2.4.1.166

raffinose + raffinose = lychnose + sucrose

488685

-

2.4.1.162

4 entries

2.4.1.166

2 entries

2.4.1.230

beta-D-glucose 1-phosphate + raffinose = 2-alpha-D-glucopyranosyl-raffinose + 2G(2-alpha-D-glucopyranosyl)2-raffinose + 2G(2-alpha-D-glucopyranosyl)3-raffinose

660528

-

2.4.1.5

sucrose + raffinose = D-fructose + ?

756970

-

2.4.1.67

1-alpha-D-galactosyl-myo-inositol + raffinose = myo-inositol + stachyose

638260, 638266, 638265, 723481, 638267, 638261, 638262, 638263, 638264, 638268

-

2.4.1.67

alpha-D-galactosyl-(1->3)-1D-myo-inositol + raffinose = myo-inositol + stachyose

737276, 736162, 736151

-

2.4.1.67

alpha-D-galactosyl-D-ononitol + raffinose = D-ononitol + stachyose

638265, 638267, 638268

-

2.4.1.67

galactinol + raffinose = ?

706231

-

2.4.1.67

galactinol + raffinose = myo-inositol + stachyose

706750

-

2.4.1.67

p-nitrophenyl-alpha-D-galactopyranoside + raffinose = p-nitrophenol + stachyose

638261

-

2.4.1.82

myo-inositol + raffinose = 1-alpha-D-galactosyl-1D-myo-inositol + sucrose

706178

-

2.4.1.82

myo-inositol + raffinose = galactinol + sucrose

756752

-

2.4.1.82

raffinose + sucrose = sucrose + raffinose

638270, 638269

-

2.4.1.9

raffinose + [(2->1)-beta-D-fructosyl]n = glucose + [(2->1)-beta-D-fructosyl]n+1

735520

-

2.4.1.9

sucrose + raffinose = ?

675837

-

2.4.1.9

sucrose + raffinose = glucose + 1-kestose

735520

-

2.4.1.67

6 entries

2.4.1.82

3 entries

2.4.1.9

3 entries

2.4.1.99

raffinose + H2O = ?

757277

-

3.2.1.154

raffinose + H2O = ?

208817, 208820

-

3.2.1.22

D-glucose + raffinose = ?

171187

-

3.2.1.22

raffinose + galactinol = stachyose + H2O

171187

-

3.2.1.22

raffinose + H2O = ?

732077

-

3.2.1.22

raffinose + H2O = alpha-D-galactose + sucrose

763192

-

3.2.1.22

raffinose + H2O = D-fructose + ?

750215

-

3.2.1.22

raffinose + H2O = D-galactose + sucrose

716494, 679586, 741951, 706750, 706231, 742095

-

3.2.1.22

raffinose + H2O = sucrose + alpha-D-galactose

714959, 715877, 656418, 171239, 655102, 705951, 695676, 713795, 655085, 713881, 171259, 656702, 654803, 715878, 654253, 696169, 680458, 705088, 714491, 715537, 656949, 655041, 655258, 656943, 678818

-

3.2.1.22

raffinose + H2O = sucrose + D-galactose

171187, 171232, 171241, 171252, 682601, 171236, 171253, 679949, 171230, 171213, 171203, 171244, 171224, 749435, 681719, 656702, 171215, 171188, 171195, 171255, 678427, 171205, 171219, 171207, 171211, 171212, 171201, 171190, 171198, 171210, 171223, 696169, 663733, 696825, 700666, 695837, 750624, 749778, 750575, 730176, 680270, 698429, 749609

-

3.2.1.22

raffinose + melibiose = stachyose + H2O

171187

-

3.2.1.22

raffinose + methanol = methyl alpha-D-galactoside + verbascose + ajugose

171187

-

3.2.1.22

raffinose + raffinose = ?

171187

-

3.2.1.22

sucrose + raffinose = planteose + D-glucose

171187

-

3.2.1.26

raffinose + H2O = alpha-D-galactosyl-1,6-alpha-D-glucose + D-fructose

663979, 665273, 665023

-

3.2.1.26

raffinose + H2O = beta-D-fructose + alpha-D-melibiose

655104, 750234

-

3.2.1.26

raffinose + H2O = beta-D-fructose + melibiose

656948, 750987, 750854, 655768, 656953, 655084, 715828, 751229, 754303

-

3.2.1.26

raffinose + H2O = D-fructose + ?

708454

-

3.2.1.26

raffinose + H2O = D-fructose + melibiose

681476, 731028, 680989, 681151, 714490, 665943, 716998, 730373, 730730, 730034, 700678, 715225

-

3.2.1.26

raffinose + H2O = fructose + melibiose

136091, 136098, 136118, 136066, 136072, 136077, 136138, 136110, 136074, 136104, 136105, 136093, 136062, 136117, 679587, 136067, 136139, 136115, 136116, 136100, 136120, 136095, 136109, 136079, 136087, 136097, 136088, 136101, 136099, 136127, 136119

-

3.2.1.26

raffinose = beta-D-fructose + melibiose

655704

-

3.2.1.22

12 entries

3.2.1.26

7 entries

3.2.1.28

raffinose + H2O = ?

666654

-

3.2.1.4

raffinose + H2O = ?

744076

-

3.2.1.48

raffinose + H2O = ?

136519

-

3.2.1.65

D(+)-raffinose + H2O = beta-D-fructose + alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranoside

655383

-

3.2.1.65

D(+)-raffinose + H2O = D-fructose + ?

655294

-

3.2.1.65

raffinose + H2O = ?

136767

-

3.2.1.7

raffinose + H2O = ?

729183

-

3.2.1.7

raffinose + H2O = D-fructose + ?

695685

-

3.2.1.7

raffinose + H2O = D-fructose + D-galactopyranosyl-(1-6)-D-glucopyranoside

656437

-

3.2.1.80

raffinose + H2O = beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside

657429, 655019, 681357

-

3.2.1.80

raffinose + H2O = beta-D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranose

655714

-

3.2.1.80

raffinose + H2O = beta-D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside

654218

-

3.2.1.80

raffinose + H2O = D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside

208817, 208820, 208818, 136519

-

3.2.1.65

3 entries

3.2.1.7

3 entries

3.2.1.80

4 entries

4.2.2.16

raffinose + D-galactopyranose = ?

665777

-

Product in Enzyme-catalyzed Reactions (16 results)

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Show Product (16 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.10

sucrose + melibiose = D-glucose + raffinose

0

-

2.4.1.10

sucrose + melibiose = raffinose + D-glucose

0

-

2.4.1.10

2 entries

2.4.1.67

stachyose + stachyose = raffinose + verbascose

638268

-

2.4.1.82

1-alpha-D-galactosyl-myo-inositol + sucrose = myo-inositol + raffinose

638270, 638269, 638271, 0

-

2.4.1.82

4-nitrophenol-alpha-D-galactopyranoside + sucrose = 4-nitrophenol + raffinose

0

-

2.4.1.82

alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose

0

-

2.4.1.82

alpha-D-galactosyl-D-ononitol + sucrose = D-ononitol + raffinose

638271

-

2.4.1.82

galactinol + sucrose = myo-inositol + raffinose

0

-

2.4.1.82

galactinol + sucrose = raffinose + ?

0

-

2.4.1.82

galactinol + sucrose = raffinose + myo-inositol

0

-

2.4.1.82

raffinose + sucrose = sucrose + raffinose

638270, 638269

-

3.2.1.22

stachyose + H2O = raffinose + D-galactose

0

-

3.2.1.22

sucrose + galactinol = raffinose + H2O

0

-

3.2.1.22

sucrose + melibiose = raffinose + D-glucose

0

-

3.2.1.22

sucrose + melibiose = raffinose + stachyose + planteose

0

-

3.2.1.22

sucrose + phenyl alpha-D-galactoside = raffinose + stachyose + planteose

0

-

2.4.1.82

8 entries

3.2.1.22

5 entries

Inhibitor in Enzyme-catalyzed Reactions (10 results)

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Show Inhibitors (10 entries)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.10

-

736891

-

2.4.1.67

competitive inhibition when the enzyme is incubated with both raffinose and D-ononitol simultaneously

638265

-

2.7.1.208

a 40fold excess of unlabeled raffinose reduces maltose uptake to about 60-70% of the control rate

674325

-

3.2.1.22

80.60% activity at 10 mM

680270

-

3.2.1.22

81.73% activity at 10 mM

680270

-

3.2.1.22

93% activity at 10 mM

680270

-

3.2.1.22

competitive with p-nitrophenyl alpha-D-galactopyranoside

656418

-

3.2.1.22

with p-nitrophenyl alpha-D-galactopyranoside as substrate

655258

-

3.2.1.23

-

171310

-

3.2.1.23

53% residual activity at 200 mM and 95% residual activity at 20 mM (purified lactase)

713677

-

3.2.1.22

5 entries

3.2.1.23

2 entries

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (28 results)

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Show Turnover Number (28 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

1.1.99.29

19.61

-

with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C

741362

3.2.1.22

0.89

-

pH 5.0, 40°C

696169

3.2.1.22

1

-

isoform Ag-II, at pH 7.0 and 36°C

749435

3.2.1.22

1.83

-

isoform Ag-I, at pH 7.0 and 36°C

749435

3.2.1.22

4.12

-

pH 5.0, 55°C, intracellular enzyme

698429

3.2.1.22

4.2

-

pH 4.5, 50°C

742095

3.2.1.22

5.2

-

mutant enzyme W336S of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C

732077

3.2.1.22

10.3

-

mutant enzyme W336N of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C

732077

3.2.1.22

32.4

-

50°C, pH 4.5

654803

3.2.1.22

46.8

-

wild-type, pH 7.4, 30°C

715537

3.2.1.22

48.1

-

mutant enzyme W336A of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C

732077

3.2.1.22

91

-

mutant A41Y, pH 7.4, 30°C

715537

3.2.1.22

109

-

pH 5, 75°C

656702

3.2.1.22

157.3

-

pH 7, 98°C

656702

3.2.1.22

180

-

wild type enzyme isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C

732077

3.2.1.22

270

-

at pH 6.0 and 40°C

763192

3.2.1.22

586

-

pH 5, 37°C

714959

3.2.1.22

832

-

mutant enzyme W336F of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C

732077

3.2.1.22

1100

-

pH 5.0, 40°C

696169

3.2.1.22

1205

-

wild type enzyme isoform AgaB, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C

732077

3.2.1.22

6946

-

at 55°C, in 50 mM citrate buffer (pH 4.2)

678427

3.2.1.22

6946

-

pH not specified in the publication, temperature not specified in the publication

741951

3.2.1.26

10.9

-

in 0.2M sodium acetate buffer (pH 5.6), at 50°C

680989

3.2.1.26

16

-

pH 5.0, 37°C

714490

3.2.1.26

118

-

37°C, pH 7.5

750234

3.2.1.26

140

-

-

136117

3.2.1.22

21 entries

3.2.1.26

4 entries

3.2.1.4

0.092

-

pH 5.0, 60°C

744076

3.2.1.7

0.5

-

-

695685

K_M Value (103 results)

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Show KM Value (103 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.1.99.29

10.79

-

with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C

741362

2.4.1.67

0.2592

-

at pH 7.0 and 25°C

736162

2.4.1.67

0.2592

-

pH 7.0, 25°C, recombinant His-tagged enzyme

736162

2.4.1.67

2.4

-

with 1-alpha-D-galactosyl-myo-inositol at 1.1 mM

638265

2.4.1.67

4.6

-

+ 1-alpha-D-galactosyl-myo-inositol

638261

2.4.1.67

5.8

-

-

638263

2.4.1.67

9.7

-

with 1-alpha-D-galactosyl-myo-inositol

638266

2.4.1.67

13.2

-

with galactosylononitol at nonsaturating level

638265

2.4.1.67

38.6

-

with 1-alpha-D-galactosyl-myo-inositol at nonsaturating level

638265

2.4.1.82

10

-

+ sucrose, exchange reaction

638269

2.4.1.82

10.9

-

pH 7.0, 45°C

756752

3.2.1.22

0.42

-

pH 5.0, 65°C

695676

3.2.1.22

0.49

-

pH 5.0, 40°C

696169

3.2.1.22

0.5

-

-

171236

3.2.1.22

0.66

-

pH 5.5, 37°C

655085

3.2.1.22

1.6

-

alpha-galactosidase II2

171195

3.2.1.22

1.61

-

at 55°C, in 50 mM citrate buffer (pH 4.2)

678427

3.2.1.22

1.61

-

pH not specified in the publication, temperature not specified in the publication

741951

3.2.1.22

1.8

-

alpha-galactosidase I

171213

3.2.1.22

2

3

pH 5.0, 40°C

696169

3.2.1.22

2.1

-

-

171259

3.2.1.22

2.1

-

pH 5, 75°C

656702

3.2.1.22

2.16

-

-

171210

3.2.1.22

2.7

-

alpha-galactosidase II

171213

3.2.1.22

3.65

-

-

171241

3.2.1.22

3.8

-

-

171255

3.2.1.22

3.8

-

wild type enzyme isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C

732077

3.2.1.22

4

-

alpha-galactosidase I

171187

3.2.1.22

4.4

-

30°C, pH 5.0

655258

3.2.1.22

4.6

-

alpha-galactosidase I

171195

3.2.1.22

4.8

-

30°C, pH 5.8

655258

3.2.1.22

5

-

alpha-galactosidase II

171187

3.2.1.22

5

-

alpha-galactosidase II1

171195

3.2.1.22

5

-

smaller form

171224

3.2.1.22

5.32

-

pH 7, 98°C

656702

3.2.1.22

5.34

-

enzyme form P2

656943

3.2.1.22

5.9

-

alpha-galactosidase II

171207

3.2.1.22

6.5

-

larger form

171224

3.2.1.22

11.3

-

50°C, pH 4.5

654803

3.2.1.22

11.8

-

-

171223

3.2.1.22

12.5

-

-

171187

3.2.1.22

12.6

-

-

171239

3.2.1.22

15

-

pH 5, 37°C

714959

3.2.1.22

17.4

-

-

171252

3.2.1.22

18

-

at 37°C, pH 8.6

705951

3.2.1.22

20.1

-

immobilized enzyme, at pH 5.0 and 40°C

750575

3.2.1.22

24.1

-

-

171239

3.2.1.22

25.9

-

-

171219

3.2.1.22

26.3

-

alpha-galactosidase III

171211

3.2.1.22

27.7

-

alpha-galactosidase I

171212

3.2.1.22

27.93

-

pH 5, 40°C

713795

3.2.1.22

29.5

-

at pH 6.0 and 40°C

763192

3.2.1.22

32.8

-

pH 5.0, 55°C, intracellular enzyme

698429

3.2.1.22

32.99

-

pH 5, 40°C

713795

3.2.1.22

36.4

-

-

171215

3.2.1.22

36.6

-

mutant enzyme W336S of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C

732077

3.2.1.22

39.62

-

pH 5.0, 40°C

700666

3.2.1.22

40

-

-

171244

3.2.1.22

47.9

-

pH 4.5, 50°C

742095

3.2.1.22

54.1

-

wild-type, pH 7.4, 30°C

715537

3.2.1.22

54.26

-

37°C

695837

3.2.1.22

71.4

-

alpha-galactosidase II

171212

3.2.1.22

80

-

-

656418

3.2.1.22

87.9

-

mutant A41Y, pH 7.4, 30°C

715537

3.2.1.22

98.1

-

alpha-galactosidase I

171207

3.2.1.22

105

-

pH 7.5, temperature not specified in the publication

716494

3.2.1.22

200

-

wild type enzyme isoform AgaB, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C

732077

3.2.1.22

205

-

mutant enzyme W336N of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C

732077

3.2.1.22

250

-

mutant enzyme W336F of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C

732077

3.2.1.22

252

-

mutant enzyme W336A of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C

732077

3.2.1.26

1.3

-

in 0.2M sodium acetate buffer (pH 5.6), at 50°C

680989

3.2.1.26

1.6

-

invertase I

136127

3.2.1.26

2.9

-

invertase I

136110

3.2.1.26

4

-

-

136091

3.2.1.26

4.5

-

pH 5.0, 40°C

655704

3.2.1.26

7.37

-

in sodium acetate buffer, 100 mM, pH 4.5, at 60°C

679587

3.2.1.26

7.6

-

enzyme form F-1

136139

3.2.1.26

9.3

-

-

136115

3.2.1.26

10.6

-

beta-FFase L, pH 5.1

136074

3.2.1.26

10.8

-

invertase II

136127

3.2.1.26

13

-

invertase III

136127

3.2.1.26

14.2

-

-

136120

3.2.1.26

15

-

-

136117

3.2.1.26

16.4

-

55°C, pH 4.6, invertase 2

751229, 754303

3.2.1.26

16.7

-

enzyme form F-2

136139

3.2.1.26

17

-

-

136100

3.2.1.26

17

-

invertase II

136110

3.2.1.26

19.6

-

soluble enzyme

136087

3.2.1.26

20.4

-

beta-FFase E, pH 5.1

136074

3.2.1.26

20.7

-

pH 7.5, alkaline invertase

136119

3.2.1.26

21.7

-

beta-FFase L, pH 3.6

136074

3.2.1.26

32.19

-

pH 5.0, 37°C

655768

3.2.1.26

46.1

-

-

136104

3.2.1.26

55.98

-

40°C, pH 4.5

750854

3.2.1.26

125

-

beta-FFase E, pH 3.6

136074

3.2.1.26

141

-

37°C, pH 7.5

750234

3.2.1.26

150

-

-

136118

3.2.1.26

150

-

exteracellular enzyme

136066

3.2.1.26

150

-

extracellular and intracellular enzyme

136072

3.2.1.26

392

-

pH 5.0, 37°C

714490

2.4.1.67

8 entries

2.4.1.82

2 entries

3.2.1.22

59 entries

3.2.1.26

30 entries

3.2.1.4

-999

-

Km value 0.011 mg/ml, pH 5.0, 60°C

744076

3.2.1.7

5.75

-

-

695685

3.2.1.80

2.7

-

pH 5.5, 50°C

681357

K_i Value (7 results)

top print hide

Show KI Value (7 entries)

EC NUMBER

KI VALUE [MM]

KI VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.4.1.10

39.9

-

inhibition of sucrose hydrolysis, pH 6.0, 60°C

736891

2.4.1.10

49

-

inhibition of sucrose hydrolysis, pH 6.0, 60°C

736891

2.4.1.10

80.8

-

inhibition of sucrose hydrolysis, pH 6.0-6.6, 60°C

736891

2.4.1.10

3 entries

2.4.1.67

17.9

-

competitive against D-ononitol

638265

3.2.1.22

9

-

30°C, pH 5.8, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate

655258

3.2.1.22

13.1

-

30°C, pH 5.0, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate

655258

3.2.1.22

25.4

-

competitive with p-nitrophenyl alpha-D-galactopyranoside

656418

3.2.1.22

3 entries

References & Links

Literature References (208)

top print hide

Show Reference (208 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

136062

7764333

Purification and characterization of beta-fructofuranosidase from Aspergillus japonicus TIT-KJ1

1993

Duan, K.J.; Sheu, D.C.; Chen, J.S.

Biosci. Biotechnol. Biochem.

57

1811-1815

136066

237205

beta-D-Fructofuranoside fructohydrolase from yeast

1975

Goldstein, A.; Lampen, J.O.

Methods Enzymol.

42C

504-511

136067

-

Partial purification and properties of invertase from Carica papaya fruits

1988

Lopez, M.E.; Vattuone, M.A.; Sampietro, A.R.

Phytochemistry

27

3077-3081

136072

4967422

Comparative study of the properties of the purified internal and external invertases from yeast

1968

Gascon, S.; Neumann, N.P.; Lampen, J.O.

J. Biol. Chem.

243

1573-1577

136074

-

Purification and some properties of two types of beta-fructofuranosidase from tomato fruit

1971

Nakagawa, H.; Kawasaki, Y.; Ogura, N.; Takehana, H.

Agric. Biol. Chem.

36

18-26

136077

8358833

Purification and characterization of an Aspergillus niger invertase and its DNA sequence

1993

Boddy, L.M.; Berges, T.; Barreau, C.; Vainstein, M.H.; Dobson, M.J.; Ballance, D.J.; Peberdy, J.F.

Curr. Genet.

24

60-66

136079

-

The occurence and properties of alkaline invertase in mature roots of sugar beets

1987

Masuda, H.; Takahashi, T.; Sugawara, S.

Agric. Biol. Chem.

51

2309-2314

136087

16664223

Wheat invertases. Characterization of cell wall-bound and soluble forms

1985

Krishnan, H.B.; Blanchette, J.T.; Okita, T.W.

Plant Physiol.

78

241-245

136088

16663453

Invertases of lilium pollen. Characterization and activity during in vitro germination

1984

Singh, M.B.; Knox, R.B.

Plant Physiol.

74

510-515

136091

16661402

Purification and some properties of cell wall-bound invertases from sugar beet seedlings and aged slices of mature roots

1980

Masuda, H.; Sugawara, S.

Plant Physiol.

66

93-96

136093

-

Purification and characterization of invertase isoenzymes from Fusarium oxysporum

1980

Nishizawa, M.; Maruyama, Y.; Nakamura, M.

Agric. Biol. Chem.

44

489-498

136095

-

An acid- and heat-stable beta-fructofuranosidase from Corticium rolfsii

1976

Sato, M.; Kaji, A.

Agric. Biol. Chem.

40

2107-2108

136097

-

Production and some properties of an extracellular invertase from Neurospora sitophila

1974

Dixon, M.M.; Fogarty, W.M.

Biochem. Soc. Trans.

2

1339-1341

136098

-

Some physocochemical properties of invertase of Candida utilis

1974

Iizuka, M.; Tsuji, Y.; Yamamoto, T.

Agric. Biol. Chem.

38

213-215

136099

-

Purification and characterization of acid invertase from cultured tobacco cells

1988

Nakamura, M.; Hagimori, M.; Matsumoto, T.

Agric. Biol. Chem.

52

3157-3158

136100

-

Acid invertase from Tropaeolum leaves

1988

Isla, M.I.; Vattuone, M.A.; Gutierrez, M.I.; Sampietro, A.R.

Phytochemistry

27

1993-1998

136101

-

Purification and properties of invertase from a glutamate-producing bacterium

1986

Yamamoto, K.; Kitamoto, Y.; Ohata, N.; Isshiki, S.; Ichikawa, Y.

J. Ferment. Technol.

64

285-291

136104

6884506

Purification and properties of the beta-fructofuranosidase from Kluyveromyces fragilis

1983

Workman, W.E.; Day, D.F.

FEBS Lett.

160

16-20

136105

-

Studies on beta-fructosidase from radish seedlings. I. Purification and partial characterization

1981

Faye, L.; Berjonneau, C.; Rollin, P.

Plant Sci. Lett.

22

77-87

136109

7396503

Purification and properties of invertases extractable from Phytophthora megasperma var. sojae mycelia

1980

West, C.; Wade, M.; McMillan III, C.; Albersheim, P.

Arch. Biochem. Biophys.

201

25-35

136110

-

Invertases in oat seedlings

1980

Pressey, R.; Avants, J.K.

Plant Physiol.

65

135-140

136115

7669814

Purification and characterization of the invertase from Pycnoporus sanguineus

1995

Quiroga, E.N.; Vattuone, M.A.; Sampietro, A.R.

Biochim. Biophys. Acta

1251

75-80

136116

10679050

Purification and characterization of invertase from Lactobacillus reuteri CRL 1100

2000

Cuezzo de Gines, S.; Maldonado, M.C.; Font de Valdez, G.

Curr. Microbiol.

40

181-184

136117

9720201

Analysis of the gene for beta-fructosidase (invertase, inulinase) of the hyperthermophilic bacterium Thermotoga maritima, and characterization of the enzyme expressed in Escherichia coli

1998

Liebl, W.; Brem, D.; Gotschlich, A.

Appl. Microbiol. Biotechnol.

50

55-64

136118

9165761

Purification and characterization of an invertase from Candida utilis: comparison with natural and recombinant yeast invertases

1997

Chavez, F.P.; Rodriguez, L.; Diaz, J.; Delgado, J.M.; Cremata, J.A.

J. Biotechnol.

53

67-74

136119

8972597

Purification and characterization of neutral and alkaline invertase from carrot

1996

Lee, H.S.; Sturm, A.

Plant Physiol.

112

1513-1522

136120

-

Properties of acid invertase purified from peach fruits

1991

Moriguchi, T.; Sanada, T.; Yamaki, S.

Phytochemistry

30

95-97

136127

-

Characterization and localization of three soluble invertase forms from Lilium longiflorum flower buds

1994

Miller, W.B.; Ranwala, A.P.

Physiol. Plant.

92

247-253

136138

-

Purification and characterization of invertase from Aspergillus niger

1995

Rubio, M.C.; Maldonado, M.C.

Curr. Microbiol.

31

80-83

136139

-

Production and characterization of raffinose-hydrolysing and invertase activities of Aspergillus fumigatus

1999

De Rezende, S.T.; Felix, C.R.

Folia Microbiol. (Praha)

44

191-195

136519

7828936

Characterization of levJ, a sucrase/fructanase-encoding gene from Actinomyces naeslundii T14V, and comparison of its product with other sucrose-cleaving enzymes

1995

Norman, J.M.; Bunny, K.L.; Giffard, P.M.

Gene

152

93-98

136767

9569612

Purification and characterization of a novel levanoctaoase-producing levanase from Pseudomonas strain K-52

1998

Kang, S.K.; Lee, S.O.; Lim, Y.S.; Jang, K.L.; Lee, T.H.

Biotechnol. Appl. Biochem.

27

159-166

171187

4561015

Biochemistry of alpha-galactosidases

1972

Dey, P.M.; Pridham, J.B.

Adv. Enzymol. Relat. Areas Mol. Biol.

36

91-130

171188

-

Raffinose

1989

Beutler, H.O.

Methods Enzym. Anal. , 3rd Ed. (Bergmeyer, H. U. , ed. )

6

90-96

171190

-

Carbohydrates

1984

Crueger, A.; Crueger, W.

Biotechnology (Kieslich, K. , ed. ) Verlag Chemie, Weinheim

6A

421-457

171195

-

alpha-Galactosidases of Vigna unguiculata

1989

Alani, S.R.; Smith, D.M.; Markakis, P.

Phytochemistry

28

2047-2051

171198

9145516

Purification, characterization and cDNA cloning of a novel alpha-Galactosidase from Mortierella vinacea

1997

Shibuya, H.; Kobayashi, H.; Sato, T.; Kim, W.S.; Yoshida, S.; Kaneko, S.; Kasamo, K.; Kusakabe, I.

Biosci. Biotechnol. Biochem.

61

592-598

171201

16347214

Production, purification and characterization of alpha-galactosidase from Monascus pilosus

1986

Wong, H.C.; Hu, C.A.; Yeh, H.L.; Su, W.; Lu, H.C.; Lin, C.F.

Appl. Environ. Microbiol.

52

1147-152

171203

6296089

Characterization of a glycoprotein alpha-Galactosidase from lentil seeds (Lens culinaris)

1983

Dey, P.M.; Campillo, E.M.D.; Lezica R.P.

J. Biol. Chem.

258

923-929

171205

3007446

Purification and characterization of alpha-galactosidase from watermelon

1986

Itoh, T.; Uda, Y.; Nakagawa, H.

J. Biochem.

99

243-250

171207

2981815

Purification and characterization of two alpha-galactosidases associated with catabolism of guar gum and other alpha-galactosides by Bacteroides ovatus

1985

Gheradini, F.; Babcock, M.; Salyers, A.A.

J. Bacteriol.

161

500-506

171210

-

Purification and enzymatic properties of alpha-galactosidase from Pycnoporus cinnabarinus

1984

Ohtakara, A.; Mitsutomi, M.; Uchida, Y.

Agric. Biol. Chem.

48

1319-1327

171211

6331399

Glycosidases induced in Aspergillus tamarii. Secreted alpha-D-galactosidase and beta-D-mannase

1984

Civas, A.; Eberhard, R.; Le Dizet, P.; Petek, F.

Biochem. J.

219

857-863

171212

6331398

Glycosidases induced in Aspergillus tamarii. Mycelial alpha-D-galactosidass

1984

Civas, A.; Eberhard, R.; Le Dizet, P.; Petek, F.

Biochem. J.

219

849-855

171213

-

Tomato alpha-Galactosidases: conversion of human type B erythrocytes to type O

1984

Pressey, R.

Phytochemistry

23

55-58

171215

16662884

Partial purification and properties of an alkaline alpha-galactosidase from mature leaves of Cucurbita pepo

1983

Gaudreault, P.R.; Webb, J.A.

Plant Physiol.

71

662-668

171219

6279579

Purification and properties of alpha-galactosidase from immature stalks of Saccharum officinarum (sugar cane)

1981

Chinen, I.; Nakamura, T.; Fukuda, N.

J. Biochem.

90

1453-1461

171223

-

Purification and some properties of alpha-galactosidase from tubers of Stachys affinis

1980

Ueno, Y.; Ikami, T.; Yamauchi, R.; Kato, K.

Agric. Biol. Chem.

44

2623-2629

171224

16661511

Characterization of alpha-Galactosidas from cucumber leaves

1980

Smart, E.L.; Pharr, D.M.

Plant Physiol.

66

731-734

171230

-

alpha-galactosidase from alfalfa seeds (Medicago, sativa L.)

1979

Itoh, T.; Shimura, S.; Adachi, S.

Agric. Biol. Chem.

43

1499-1504

171232

216315

alpha-Galactosidase (melibiase) from Saccharomyces carlsbergensis: structural and kinetic properties

1978

Lazo, P.S.; Ochoa, A.G.; Gascon, S.

Arch. Biochem. Biophys.

191

316-324

171236

14112

Glycoprotein enzymes secreted by Aspergillus niger: purification and properties of alpha-galactosidase

1977

Adya, S.; Elbein, A.D.

J. Bacteriol.

129

850-856

171239

-

Properties of alpha-galactosidases of alkalophilic bacteria

1976

Akiba, T.; Horikoshi, K.

Agric. Biol. Chem.

40

1851-1855

171241

-

Purification ans properties of alpha-galactosidase from Escherichia coli subsp. communior IAM 1272

1976

Kawamura, S.; Kasai, T.; Tanusi, S.

Agric. Biol. Chem.

40

641-648

171244

-

Isolation and characterization of alpha-Galactosidas from Lens esculanta

1994

Dey, P.M.; Wallenfels, K.

Eur. J. Biochem.

50

107-112

171252

10418141

Thermostable alpha-Galactosidas from Bacillus stearothermophilus NUB3621: cloning, sequencing and characterization

1999

Fridjonsson, O.; Watzlawick, H.; Gehweiler, A.; Mattes, R.

FEMS Microbiol. Lett.

176

147-153

171253

10613841

Regulation of the alpha-galactosidase activity in Streptococcus pneumoniae: characterization of the raffinose utilization system

1999

Rosenow, C.; Maniar, M.; Trias, J.

Genome Res.

9

1189-1197

171255

8390816

Conditions of formation, purification, and characterization of an alpha-galactosidase of Trichoderma reesei RUT C-30

1993

Zeilinger, S.; Kristufek, D.; Arisan-Atac, I.; Hodits, R.; Kubicek, C.P.

Appl. Biochem. Biotechnol.

59

1347-1353

171259

9741105

Properties of an alpha-Galactosidas, and structure of its gene galA, within an alpha- and beta-galactoside utilization gene cluster of the hyperthermophilic bacterium Thermotoga maritima

1998

Liebl, W.; Wagner, B.; Schellhase, J.

Syst. Appl. Microbiol.

21

1-11

171310

6787981

Purification and characterization of beta-galactosidase from a strain of Bacillus coagulans

1981

Levin, R.E.; Mahoney, R.R.

Antonie van Leeuwenhoek

47

53-64

208817

3308844

Expression, purification, and characterization of an exo-beta-D-fructosidase of Streptococcus mutans

1987

Burne, R.A.; Schilling, K.; Bowen, W.H.; Yasbin, R.E.

J. Bacteriol.

169

4507-4517

208818

3965399

Purification and preliminary characterization of exo-beta-D-fructosidase in Streptococcus salivarius KTA-19

1985

Takahashi, N.; Mizuno, F.; Takamori, K.

Infect. Immun.

47

271-276

208820

1522814

Characterization of an exo-beta-D-fructosidase from Streptococcus mutants ingbritt

1992

Igarashi, T.; Yakamoto, A.; Goto, N.

Microbiol. Immunol.

36

643-647

285900

-

A method for the assay of glucose isomerase activity in complex fermentation mixtures

1980

Boguslawski, G.; Bertch, S.W.

J. Appl. Biochem.

2

367-372

389851

-

Expression and characterization of a recombinant Fusarium spp. galactose oxidase

2000

Xu, F.; Golightly, E.J.; Schneider, P.; Berka, R.M.; Brown, K.M.; Johnstone, J.A.; Baker, D.H.; Fuglsang, C.C.; Brown, S.H.; Svendsen, A.; Klotz, A.V.

Appl. Biochem. Biotechnol.

88

2332

389862

3813549

Purification and characterization of intracellular galactose oxidase from Dactylium dendroides

1987

Mendoca, M.H.; Zancan, G.T.

Arch. Biochem. Biophys.

252

507-514

389863

3733747

Re-examination of the products of the action of galactose oxidase. Evidence for the conversion of raffinose to 6''-carboxyraffinose

1986

Kelleher, F.M.; Bhavanandan, V.P.

J. Biol. Chem.

261

11045-11048

389864

-

Catalytic properties of Gibberella galactose oxidase

1985

Aisaka, K.; Uwajima, T.; Terada, O.

Agric. Biol. Chem.

49

1201-1202

389866

-

Kinetic properties of galactose oxidase from Gibberella fujikuroi

1984

Aisaka, K.; Uwajima, T.; Terada, O.

Agric. Biol. Chem.

48

1425-1431

389881

-

Galactose oxidase of Polyporus circinatus

1966

Amaral, D.; Kelly-Falcoz, F.; Horecker, B.L.

Methods Enzymol.

9

87-92

389882

13863403

The D-galactose oxidase of Polyporus circinatus

1962

Avigad, G.; Amaral, D.; Asensio, C.; Horecker, B.L.

J. Biol. Chem.

237

2736-2743

389892

-

Purification und properties of 3-ketosucrose-forming enzyme from the cells of Agrobacterium tumefaciens

1967

Hayano, K.; Fukui, S.

J. Biol. Chem.

242

3665-3672

389893

165382

Hexopyranoside: cytochrome c oxidoreductase from Agrobacterium

1975

Van Beeumen, J.; De Ley, J.

Methods Enzymol.

41

153-158

488316

-

The mode of synthesis of levan by Bacillus subtilis levansucrase

1985

Yamamoto, S.; Iizuka, M.; Tanaka, T.; Yamamoto, T.

Agric. Biol. Chem.

49

343-349

488324

3236222

Detection and characterization of a levansucrase and a sucrase in Pseudomonas syringae pv. phaseolicola

1988

Sauerstein, J.; Reuter, G.

J. Basic Microbiol.

28

667-672

488343

12359071

Identification of functionally important amino acid residues in Zymomonas mobilis levansucrase

2002

Yanase, H.; Maeda, M.; Hagiwara, E.; Yagi, H.; Taniguchi, K.; Okamoto, K.

J. Biochem.

132

565-572

488677

-

Trihalo-trideoxy galactosucrose compounds

1985

Rathbone, E.B.; Hacking, A.J.; Cheetham, P.S.J.

UK Patent Application

GB21

45080 A

488685

24253101

Physiology and biosynthesis of lychnose in Cerastium arvense

1984

Hopf, H.; Gruber, G.; Zinn, A.; Kandler, O.

Planta

162

283-288

638260

-

Die Biosynthese der Stachyose

1967

Tanner, W.

Ber. Dtsch. Bot. Ges.

80

111

638261

-

Stachyose synthesis in leaves of Cucurbita pepo

1981

Gaudreault, P.R.; Webb, J.A.

Phytochemistry

20

2629-2633

638262

24232761

Are sucrosyl-oligosaccharides synthesized in mesophyll protoplasts of mature leaves of Cucurbita melo ?

1986

Schmitz, K.; Holthaus, U.

Planta

169

529-535

638263

12232288

Metabolism of the raffinose family oligosaccharides in leaves of Ajuga reptans L.. Cold acclimation, translocation, and sink to source transition: discovery of chain elongation enzyme

1994

Bachmann, M.; Matile, P.; Keller, F.

Plant Physiol.

105

1335-1345

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