Ligand raffinose

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

Basic Ligand Information

Molecular Structure
Picture of raffinose (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C18H32O16
raffinose
MUPFEKGTMRGPLJ-ZQSKZDJDSA-N
Synonyms:
D(+)-raffinose, D-raffinose

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (9 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
raffinose + O2 = ? + H2O2
-
show the reaction diagram
raffinose + H2O = sucrose + D-fructose
-

In Vivo Product in Enzyme-catalyzed Reactions (2 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE

Substrate in Enzyme-catalyzed Reactions (68 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
D-raffinose + NADH = ?
-
show the reaction diagram
D-raffinose + 2,6-dichlorophenolindophenol = ? + reduced 2,6-dichlorophenolindophenol
-
show the reaction diagram
raffinose + acceptor = ?
-
show the reaction diagram
raffinose + ferrocenium ion = ?
-
show the reaction diagram
sucrose + raffinose = ?
-
show the reaction diagram
beta-D-glucose 1-phosphate + raffinose = 2-alpha-D-glucopyranosyl-raffinose + 2G(2-alpha-D-glucopyranosyl)2-raffinose + 2G(2-alpha-D-glucopyranosyl)3-raffinose
-
show the reaction diagram
sucrose + raffinose = D-fructose + ?
-
show the reaction diagram
raffinose + H2O = ?
-
show the reaction diagram
raffinose + H2O = ?
-
show the reaction diagram
raffinose + H2O = ?
-
show the reaction diagram
raffinose + H2O = ?
-
show the reaction diagram
raffinose + H2O = ?
-
show the reaction diagram
raffinose + D-galactopyranose = ?
-

Product in Enzyme-catalyzed Reactions (16 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
stachyose + stachyose = raffinose + verbascose
-

Inhibitor in Enzyme-catalyzed Reactions (10 results)

COMMENTARY
EC NUMBER
LITERATURE
ENZYME 3D STRUCTURE
competitive inhibition when the enzyme is incubated with both raffinose and D-ononitol simultaneously
-
a 40fold excess of unlabeled raffinose reduces maltose uptake to about 60-70% of the control rate
-
80.60% activity at 10 mM
-
81.73% activity at 10 mM
-
93% activity at 10 mM
-
competitive with p-nitrophenyl alpha-D-galactopyranoside
-
with p-nitrophenyl alpha-D-galactopyranoside as substrate
-
53% residual activity at 200 mM and 95% residual activity at 20 mM (purified lactase)
-

3D Structure of Enzyme-Ligand-Complex (PDB) (1 result)

EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (27 results)

COMMENTARY
EC NUMBER
LITERATURE
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C
19.61
-
pH 4.5, 50°C
4.2
-
wild-type, pH 7.4, 30°C
46.8
-
wild type enzyme isoform AgaB, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C
1205
-
wild type enzyme isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C
180
-
pH not specified in the publication, temperature not specified in the publication
6946
-
pH 7, 98°C
157.3
-
pH 5.0, 55°C, intracellular enzyme
4.12
-
pH 5.0, 40°C
pH 5, 75°C
109
-
pH 5, 37°C
586
-
mutant enzyme W336S of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C
5.2
-
mutant enzyme W336N of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C
10.3
-
mutant enzyme W336F of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C
832
-
mutant enzyme W336A of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C
48.1
-
mutant A41Y, pH 7.4, 30°C
91
-
isoform Ag-II, at pH 7.0 and 36°C
1
-
isoform Ag-I, at pH 7.0 and 36°C
1.83
-
at 55°C, in 50 mM citrate buffer (pH 4.2)
6946
-
50°C, pH 4.5
32.4
-
-
140
-
pH 5.0, 37°C
16
-
in 0.2M sodium acetate buffer (pH 5.6), at 50°C
10.9
-
37°C, pH 7.5
118
-
pH 5.0, 60°C
0.092
-
-
0.5
-

KM Value (102 results)

COMMENTARY
EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
LITERATURE
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C
10.79
-
-
5.8
-
+ 1-alpha-D-galactosyl-myo-inositol
4.6
-
at pH 7.0 and 25°C
0.2592
-
pH 7.0, 25°C, recombinant His-tagged enzyme
0.2592
-
with 1-alpha-D-galactosyl-myo-inositol
9.7
-
with 1-alpha-D-galactosyl-myo-inositol at 1.1 mM
2.4
-
with 1-alpha-D-galactosyl-myo-inositol at nonsaturating level
38.6
-
with galactosylononitol at nonsaturating level
13.2
-
+ sucrose, exchange reaction
10
-
pH 7.0, 45°C
10.9
-
30°C, pH 5.0
4.4
-
30°C, pH 5.8
4.8
-
37°C
54.26
-
50°C, pH 4.5
11.3
-
alpha-galactosidase I
alpha-galactosidase II
alpha-galactosidase II1
5
-
alpha-galactosidase II2
1.6
-
alpha-galactosidase III
26.3
-
at 37°C, pH 8.6
18
-
at 55°C, in 50 mM citrate buffer (pH 4.2)
1.61
-
enzyme form P2
5.34
-
immobilized enzyme, at pH 5.0 and 40°C
20.1
-
larger form
6.5
-
mutant A41Y, pH 7.4, 30°C
87.9
-
mutant enzyme W336A of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C
252
-
mutant enzyme W336F of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C
250
-
mutant enzyme W336N of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C
205
-
mutant enzyme W336S of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C
36.6
-
pH 4.5, 50°C
47.9
-
pH 5, 37°C
15
-
pH 5, 40°C
pH 5, 75°C
2.1
-
pH 5.0, 40°C
pH 5.0, 55°C, intracellular enzyme
32.8
-
pH 5.0, 65°C
0.42
-
pH 5.5, 37°C
0.66
-
pH 7, 98°C
5.32
-
pH 7.5, temperature not specified in the publication
105
-
pH not specified in the publication, temperature not specified in the publication
1.61
-
smaller form
5
-
wild type enzyme isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C
3.8
-
wild type enzyme isoform AgaB, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C
200
-
wild-type, pH 7.4, 30°C
54.1
-
37°C, pH 7.5
141
-
40°C, pH 4.5
55.98
-
55°C, pH 4.6, invertase 2
16.4
-
beta-FFase E, pH 3.6
125
-
beta-FFase E, pH 5.1
20.4
-
beta-FFase L, pH 3.6
21.7
-
beta-FFase L, pH 5.1
10.6
-
enzyme form F-1
7.6
-
enzyme form F-2
16.7
-
exteracellular enzyme
150
-
extracellular and intracellular enzyme
150
-
in 0.2M sodium acetate buffer (pH 5.6), at 50°C
1.3
-
in sodium acetate buffer, 100 mM, pH 4.5, at 60°C
7.37
-
invertase I
invertase II
invertase III
13
-
pH 5.0, 37°C
pH 5.0, 40°C
4.5
-
pH 7.5, alkaline invertase
20.7
-
soluble enzyme
19.6
-
Km value 0.011 mg/ml, pH 5.0, 60°C
-999
-
-
5.75
-
pH 5.5, 50°C
2.7
-

Ki Value (7 results)

COMMENTARY
EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
LITERATURE
inhibition of sucrose hydrolysis, pH 6.0, 60°C
inhibition of sucrose hydrolysis, pH 6.0-6.6, 60°C
80.8
-
competitive against D-ononitol
17.9
-
30°C, pH 5.0, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate
13.1
-
30°C, pH 5.8, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate
9
-
competitive with p-nitrophenyl alpha-D-galactopyranoside
25.4
-

References & Links

Links to other databases for raffinose

ChEBI
PubChem
-
PubChem