Ligand raffinose

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Basic Ligand Information

Molecular Structure
Picture of raffinose (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C18H32O16
raffinose
MUPFEKGTMRGPLJ-ZQSKZDJDSA-N
Synonyms:
D(+)-raffinose, D-raffinose

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (10 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
raffinose + H2O = sucrose + D-fructose
show the reaction diagram
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In Vivo Product in Enzyme-catalyzed Reactions (2 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE

Substrate in Enzyme-catalyzed Reactions (70 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
D-raffinose + NADH = ?
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D-raffinose + 2,6-dichlorophenolindophenol = ? + reduced 2,6-dichlorophenolindophenol
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raffinose + acceptor = ?
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raffinose + ferrocenium ion = ?
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sucrose + raffinose = ?
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beta-D-glucose 1-phosphate + raffinose = 2-alpha-D-glucopyranosyl-raffinose + 2G(2-alpha-D-glucopyranosyl)2-raffinose + 2G(2-alpha-D-glucopyranosyl)3-raffinose
show the reaction diagram
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sucrose + raffinose = D-fructose + ?
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raffinose + H2O = ?
show the reaction diagram
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raffinose + H2O = ?
show the reaction diagram
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raffinose + H2O = ?
show the reaction diagram
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raffinose + H2O = ?
show the reaction diagram
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raffinose + H2O = ?
show the reaction diagram
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raffinose + D-galactopyranose = ?
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Product in Enzyme-catalyzed Reactions (16 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
stachyose + stachyose = raffinose + verbascose
show the reaction diagram
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Inhibitor in Enzyme-catalyzed Reactions (10 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
competitive inhibition when the enzyme is incubated with both raffinose and D-ononitol simultaneously
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a 40fold excess of unlabeled raffinose reduces maltose uptake to about 60-70% of the control rate
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3D Structure of Enzyme-Ligand-Complex (PDB) (1 result)

EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (27 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
19.61
-
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C
0.092
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pH 5.0, 60°C
0.5
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KM Value (102 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
10.79
-
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C
-999
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Km value 0.011 mg/ml, pH 5.0, 60°C
5.75
-
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2.7
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pH 5.5, 50°C

Ki Value (7 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
17.9
-
competitive against D-ononitol

References & Links

Links to other databases for raffinose

ChEBI
PubChem
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PubChem