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Ligand 2-methylacetoacetate

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (4 results)

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Show Substrate (4 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.280

2-methyl-3-oxobutanoate + NADPH + H+ = (2R,3S)-3-hydroxy-2-methylbutanoate + NADP+

639094

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1.1.1.86

2-methylacetoacetate + NADPH + H+ = 3-hydroxy-2-methylbutanoate + NADP+

738989

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2.3.1.247

DL-3-aminobutanoyl-CoA + 2-methylacetoacetate = (5S)-5-amino-2-methyl-3-oxohexanoate + acetyl-CoA

3378

-

2.8.3.9

butanoyl-CoA + 2-methylacetoacetate = butanoate + 2-methylacetoacetyl-CoA

643838

-

Product in Enzyme-catalyzed Reactions (2 results)

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Show Product (2 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.30

2-methyl-3-hydroxybutyrate + NAD+ = 2-methylacetoacetate + NADH

286537

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6.4.1.6

2-butanone + CO2 + ATP + H2O = 2-methyl-3-oxobutanoate + AMP + phosphate

-

-

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (9 results)

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Show Turnover Number (9 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

1.1.1.86

0.4

-

pH 8.0 25°C

738989

1.1.1.86

0.5

-

pH 8.0 25°C

738989

1.1.1.86

0.6

-

pH 8.0 25°C

738989

1.1.1.86

0.67

-

pH 8.0 25°C

738989

1.1.1.86

1

-

pH 8.0 25°C

738989

1.1.1.86

1.05

-

pH 8.0 25°C

738989

1.1.1.86

1.2

-

pH 8.0 25°C

738989

1.1.1.86

1.8

-

pH 8.0 25°C

738989

1.1.1.86

2

-

pH 8.0 25°C

738989

1.1.1.86

9 entries

K_M Value (7 results)

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Show KM Value (7 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.1.1.280

5.6

-

-

639094

1.1.1.86

30

-

pH 8.0 25°C

738989

1.1.1.86

35

-

pH 8.0 25°C

738989

1.1.1.86

40

-

pH 8.0 25°C

738989

1.1.1.86

45

-

pH 8.0 25°C

738989

1.1.1.86

50

-

pH 8.0 25°C

738989

1.1.1.86

60

-

pH 8.0 25°C

738989

1.1.1.86

6 entries

References & Links

Literature References (5)

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Show Reference (5 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

3378

6811551

Pathway of lysine degradation in Fusobacterium nucleatum

1982

Barker, H.A.; Kahn, J.M.; Hedrick, L.

J. Bacteriol.

152

201-207

286537

-

Substrate analogue studies of the specificity and catalytic mechanism of D-3-hydroxybutyrate dehydrogenase

1978

Kluger, R.; Nakaoka, K.; Tsui, W.C.

J. Am. Chem. Soc.

100

7388-7392

639094

-

Purification and characterization of ethyl 2-methyl-3-oxobutanoate reductase from Klebsiella pneumoniae IFO3319

1996

Miya, H.; Kawada, M.; Sugiyama, Y.

Biosci. Biotechnol. Biochem.

60

760-764

643838

624727

Butyryl-CoA:acetoacetate CoA-transferase from a lysine-fermenting Clostridium

1978

Barker, H.A.; Jemg, I.M.; Neff, N.; Robertson, J.M.; Tam, F.K.; Hosaka, S.

J. Biol. Chem.

253

1219-1225

738989

25296650

Molecular annotation of ketol-acid reductoisomerases from Streptomyces reveals a novel amino acid biosynthesis interlock mediated by enzyme promiscuity

2015

Verdel-Aranda, K.; Lopez-Cortina, S.T.; Hodgson, D.A.; Barona-Gomez, F.

Microb. Biotechnol.

8

239-252

Links to other databases for 2-methylacetoacetate

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Show Links (1 entry)