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Ligand beta-mannobiose

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (3 results)

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Show Natural Substrate (3 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

5.1.3.11

4-O-beta-D-mannopyranosyl-beta-D-mannopyranose = 4-O-beta-D-mannopyranosyl-D-glucose

747317

-

5.1.3.11

beta-1,4-mannobiose = 4-O-beta-D-mannopyranosyl-D-glucose

748593

-

5.1.3.11

beta-mannobiose = 4-O-beta-D-mannopyranosyl-D-glucose

702762

-

5.1.3.11

3 entries

In Vivo Product in Enzyme-catalyzed Reactions (2 results)

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Show Natural Product (2 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.2.1.21

(1-4)-beta-D-glucosyl-D-mannobiose + H2O = beta-D-glucose + D-mannobiose

326316

-

3.2.1.24

Manalpha(1-4)Manbeta(1-4)Man + H2O = alpha-D-mannopyranose + Manbeta(1-4)Man

135994

-

Substrate in Enzyme-catalyzed Reactions (11 results)

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Show Substrate (11 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.319

beta-D-mannopyranosyl-(1->4)-D-mannopyranose + alpha-D-mannose 1-phosphate = beta-D-mannopyranosyl-(1->4)-beta-D-mannopyranosyl-(1->4)-D-mannopyranose + phosphate

725502

-

2.4.1.319

beta-D-mannopyranosyl-(1->4)-D-mannopyranose + phosphate = D-mannose + alpha-D-mannose 1-phosphate

725502

-

2.4.1.319

[(1->4)-beta-D-mannosyl]2 + alpha-D-mannose 1-phosphate = [(1->4)-beta-D-mannosyl]3 + phosphate

725502

-

2.4.1.319

[(1->4)-beta-D-mannosyl]2 + phosphate = D-mannose + alpha-D-mannose 1-phosphate

725502

-

2.4.1.319

4 entries

3.1.1.72

(mannose)2 + triacetin = ?

650392

-

3.2.1.25

beta1-4-mannobiose + H2O = ?

136038, 136041

-

3.2.1.25

Manbeta1-4Man + H2O = D-mannose

136028

-

5.1.3.11

4-O-beta-D-mannopyranosyl-beta-D-mannopyranose = 4-O-beta-D-mannopyranosyl-D-glucose

747317

-

5.1.3.11

4-O-beta-D-mannopyranosyl-beta-D-mannopyranose = beta-D-mannopyranosyl-(1->4)-D-glucose

747318

-

5.1.3.11

beta-1,4-mannobiose = 4-O-beta-D-mannopyranosyl-D-glucose

748593, 748409

-

5.1.3.11

beta-mannobiose = 4-O-beta-D-mannopyranosyl-D-glucose

702762

-

3.2.1.25

2 entries

5.1.3.11

4 entries

Product in Enzyme-catalyzed Reactions (20 results)

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Show Product (20 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.319

beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate = beta-D-mannopyranosyl-(1->4)-D-mannopyranose + alpha-D-mannose 1-phosphate

0

-

2.4.1.319

beta-D-mannopyranosyl-(1->4)-beta-D-mannopyranosyl-(1->4)-D-mannopyranose + phosphate = beta-D-mannopyranosyl-(1->4)-D-mannopyranose + alpha-D-mannose 1-phosphate

0

-

2.4.1.319

[(1->4)-beta-D-mannosyl]3 + phosphate = [(1->4)-beta-D-mannosyl]2 + alpha-D-mannose 1-phosphate

0

-

2.4.1.319

3 entries

3.2.1.21

(1-4)-beta-D-glucosyl-D-mannobiose + H2O = beta-D-glucose + D-mannobiose

326316

-

3.2.1.24

Manalpha(1-4)Manbeta(1-4)Man + H2O = alpha-D-mannopyranose + Manbeta(1-4)Man

135994

-

3.2.1.78

Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O = 2 Man-beta-1,4-Man

0

1qnr, 3D-view

3.2.1.78

2,4-dinitrophenyl-beta-mannobioside + H2O = 2,4-dinitrophenol + beta-mannobiose

0

1qnr, 3D-view

3.2.1.78

Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O = Man-beta-1,4-Man-beta-1,4-Man + Man-beta-1,4-Man

0

1qnr, 3D-view

3.2.1.78

Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O = Man-beta-1,4-Man + Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man

0

1qnr, 3D-view

3.2.1.78

Manbeta(1-4)Manbeta(1-4)Man + H2O = mannose + Manbeta(1-4)Man

0

1qnr, 3D-view

3.2.1.78

Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O = Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + mannose

0

1qnr, 3D-view

3.2.1.78

Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O = Manbeta(1-4)Manbeta(1-4)Man + mannose + Manbeta(1-4)Man

0

1qnr, 3D-view

3.2.1.78

Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O = mannose + Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Man

0

1qnr, 3D-view

3.2.1.78

Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O = Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Man

0

1qnr, 3D-view

3.2.1.78

Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O = Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + mannose

0

1qnr, 3D-view

3.2.1.78

Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man-ol + H2O = D-mannitol + Manbeta(1-4)Man-ol + D-mannose + Manbeta(1-4)Manbeta(1-4)Man-ol + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man-ol + Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man-ol + Manbeta(1-4)Manbeta(1-4)Man

0

1qnr, 3D-view

3.2.1.78

Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man-ol + H2O = Manbeta(1-4)Man-ol + Manbeta(1-4)Manbeta(1-4)Man + mannose + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man-ol + Manbeta(1-4)Man

0

1qnr, 3D-view

3.2.1.78

Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O = Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Man

0

1qnr, 3D-view

3.2.1.78

Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O = Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Man + mannose + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man

0

1qnr, 3D-view

3.2.1.78

Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man-ol + H2O = mannose + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man-ol + Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Man-ol + Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man

0

1qnr, 3D-view

3.2.1.78

15 entries

3D Structure of Enzyme-Ligand-Complex (PDB) (1 result)

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Show 3D Structure of Enzyme-Ligand-Complex (PDB) (1 entry)

EC NUMBER

ENZYME 3D STRUCTURE

3.2.1.78

1qnr, 3D-view

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (9 results)

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Show Turnover Number (9 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

2.4.1.319

7.06

-

pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate

725502

2.4.1.319

7.06

-

pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction

725502

2.4.1.319

45.1

-

pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate

725502

2.4.1.319

45.1

-

pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction

725502

5.1.3.11

35.8

-

pH 7.5, 30°C, recombinant enzyme

747318

5.1.3.11

102

-

pH 7.5, 30°C, recombinant enzyme

747318

5.1.3.11

102

-

pH 8.0, 40°C, recombinant enzyme

747317

5.1.3.11

104

-

pH 7.5, 30°C, recombinant enzyme

747318

5.1.3.11

201

-

pH 7.5, 30°C, recombinant enzyme

747318

2.4.1.319

4 entries

5.1.3.11

5 entries

K_M Value (10 results)

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Show KM Value (10 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.4.1.319

30.7

-

pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate

725502

2.4.1.319

30.7

-

pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction

725502

2.4.1.319

44.5

-

pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate

725502

2.4.1.319

44.5

-

pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction

725502

5.1.3.11

3.5

-

pH 7.5, 30°C, recombinant enzyme

747318

5.1.3.11

4.03

-

pH 7.5, 30°C, recombinant enzyme

747318

5.1.3.11

4.03

-

pH 8.0, 40°C, recombinant enzyme

747317

5.1.3.11

5.25

-

pH 7.5, 30°C, recombinant enzyme

747318

5.1.3.11

5.51

-

pH 7.5, 30°C, recombinant enzyme

747318

5.1.3.11

8.74

-

pH 7.5, 30°C, recombinant enzyme

747318

2.4.1.319

4 entries

5.1.3.11

6 entries

References & Links

Literature References (12)

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Show Reference (12 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

135994

4690228

Further purification and characterization of alpha-mannosidase from hog kidney

1973

Okumura, T.; Yamashina, I.

J. Biochem.

73

131-138

136028

-

Action of beta-mannosidases on O-beta-D-mannopyranosyl-(1->2)-D-mannose

1989

Takegawa, K.; Miki, S.; Osaka, F.; Jikibara, T.; Iwahara, S.

Agric. Biol. Chem.

53

1179-1180

136038

26672

Purification and characterization of beta-D-mannosidase and beta-N-acetyl-D-hexosaminidase of Tremella fuciformis

1978

Sone, Y.; Misaki, A.

J. Biochem.

83

1135-1144

136041

6478

beta-Mannosidase from the mushroom Polyporus sulfureus

1976

Wan, C.C.; Muldrey, J.E.; Li, S.C.; Li, Y.T.

J. Biol. Chem.

251

4384-4388

326316

-

Purification of beta-D-glucosidase from Aspergillus niger

1988

McCleary, B.V.; Harrington, J.

Methods Enzymol.

160

575-583

650392

14572903

Transacetylations to carbohydrates catalyzed by acetylxylan esterase in the presence of organic solvent

2003

Biely, P.; Wong, K.K.; Suckling, I.D.; Spanikova, S.

Biochim. Biophys. Acta

1623

62-71

702762

19202279

Identification of the cellobiose 2-epimerase gene in the genome of Bacteroides fragilis NCTC 9343

2009

Senoura, T.; Taguchi, H.; Ito, S.; Hamada, S.; Matsui, H.; Fukiya, S.; Yokota, A.; Watanabe, J.; Wasaki, J.; Ito, S.

Biosci. Biotechnol. Biochem.

73

400-406

725502

23093406

Metabolic mechanism of mannan in a ruminal bacterium, Ruminococcus albus, involving two mannoside phosphorylases and cellobiose 2-epimerase: discovery of a new carbohydrate phosphorylase, beta-1,4-mannooligosaccharide phosphorylase

2012

Kawahara, R.; Saburi, W.; Odaka, R.; Taguchi, H.; Ito, S.; Mori, H.; Matsui, H.

J. Biol. Chem.

287

42389-42399

747317

25704402

Functional reassignment of Cellvibrio vulgaris EpiA to cellobiose 2-epimerase and an evaluation of the biochemical functions of the 4-O-beta-D-mannosyl-D-glucose phosphorylase-like protein, UnkA

2015

Saburi, W.; Tanaka, Y.; Muto, H.; Inoue, S.; Odaka, R.; Nishimoto, M.; Kitaoka, M.; Mori, H.

Biosci. Biotechnol. Biochem.

79

969-977

747318

27031293

Functions, structures, and applications of cellobiose 2-epimerase and glycoside hydrolase family 130 mannoside phosphorylases

2016

Saburi, W.

Biosci. Biotechnol. Biochem.

80

1294-1305

748409

-

Characterization of an epilactose-producing cellobiose 2-epimerase from Thermoanaerobacterium saccharolyticum

2015

Chen, Q.; Zhang, W.; Zhang, T.; Jiang, B.; Mu, W.

J. Mol. Catal. B

116

39-44

748593

29233137

Rational modification of substrate binding site by structure-based engineering of a cellobiose 2-epimerase in Caldicellulosiruptor saccharolyticus

2017

Park, A.R.; Kim, J.S.; Jang, S.W.; Park, Y.G.; Koo, B.S.; Lee, H.C.

Microb. Cell Fact.

16

224

Links to other databases for beta-mannobiose

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