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Ligand 2-deoxy-scyllo-inosamine Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Basic Ligand Information Molecular Structure
C6 H1 3 NO4
2-deoxy-scyllo-inosamine
QXQNRSUOYNMXDL-KGJVWPDLSA-N
Roles as Enzyme Ligand
In Vivo Product in Enzyme-catalyzed Reactions (2 results)Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
L-glutamine + 2-deoxy-scyllo-inosose = 2-oxoglutaramate + 2-deoxy-scyllo-inosamine
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2-deoxy-scyllo-inosose + L-glutamine = 2-deoxy-scyllo-inosamine + 2-ketoglutaramate
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Substrate in Enzyme-catalyzed Reactions (4 results)
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2-deoxy-scyllo-inosamine + NAD+ = 3-amino-2,3-dideoxy-scyllo-inosose + NADH + H+
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2-deoxy-scyllo-inosamine + NADP+ = 3-amino-2,3-dideoxy-scyllo-inosose + NADPH + H+
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2-deoxy-scyllo-inosamine + S-adenosyl-L-methionine = 3-amino-2,3-dideoxy-scyllo-inosose + 5'-deoxyadenosine + L-methionine
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2-deoxy-scyllo-inosamine + S-adenosyl-L-methionine = 3-amino-2,3-dideoxy-scyllo-inosose + CO2 + L-methionine + 5'-deoxyadenosine
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Product in Enzyme-catalyzed Reactions (3 results)
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L-glutamine + 2-deoxy-scyllo-inosose = 2-oxoglutaramate + 2-deoxy-scyllo-inosamine
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2-deoxy-scyllo-inosose + L-glutamine = 2-deoxy-scyllo-inosamine + 2-ketoglutaramate
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2-deoxy-scyllo-inosose + L-glutamine = 2-deoxy-scyllo-inosamine + 2-oxoglutaramate
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Inhibitor in Enzyme-catalyzed Reactions (2 results)
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uncompetitive substrate inhibition
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Enzyme Kinetic Parameters
kcat Value (Turnover Number) (2 results)
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0.038
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in 50 mM HEPES-NaOH (pH 8.0) in the presence of 10 mM sodium dithionite at 28°C
KM Value (2 results)
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0.022
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in 50 mM HEPES-NaOH (pH 8.0) in the presence of 10 mM sodium dithionite at 28°C
References & Links Literature References (4)
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Mechanistic study on the reaction of a radical SAM dehydrogenase BtrN by electron paramagnetic resonance spectroscopy
2008
Yokoyama, K.; Ohmori, D.; Kudo, F.; Eguchi, T.
Biochemistry
47
8950-8960
Characterization and mechanistic study of a radical SAM dehydrogenase in the biosynthesis of butirosin
2007
Yokoyama, K.; Numakura, M.; Kudo, F.; Ohmori, D.; Eguchi, T.
J. Am. Chem. Soc.
129
15147-15155
Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773
2005
Kudo, F.; Yamamoto, Y.; Yokoyama, K.; Eguchi, T.; Kakinuma, K.
J. Antibiot.
58
766-774
Electrochemical resolution of the [4Fe-4S] centers of the AdoMet radical enzyme BtrN: evidence of proton coupling and an unusual, low-potential auxiliary cluster
2015
Maiocco, S.J.; Grove, T.L.; Booker, S.J.; Elliott, S.J.
J. Am. Chem. Soc.
137
8664-8667
Links to other databases for 2-deoxy-scyllo-inosamine
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