Ligand N-palmitoyl-phosphatidylethanolamine

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Basic Ligand Information

Molecular Structure

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Molecular Formula

BRENDA Name

InChIKey

C₂₃H₄₂NO₉PR₂

N-palmitoyl-phosphatidylethanolamine

IVLBHBFTRNVIAP-MEGGAXOGSA-N

Synonyms:

N-palmitoylphosphatidylethanolamine

Pathway Source

Pathways

MetaCyc

palmitoyl ethanolamide biosynthesis

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (3 results)

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EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.1.4.4

N-palmitoyl-phosphatidylethanolamine + H2O = phosphatidic acid + N-palmitoylethanolamine

680640, 650338, 682434

3.1.4.54

N-palmitoylphosphatidylethanolamine + H2O = N-palmitoylethanolamine + phosphatidate

666213, 710098

3.1.4.54

N-palmitoylphosphatidylethanolamine + H2O = N-palmitoylethanolamine + phosphatidic acid

709610

3.1.4.54

2 entries

Product in Enzyme-catalyzed Reactions (2 results)

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EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.1.4.54

1,2-dioleoyl-sn-glycero-3-phospho(N-palmitoyl)ethanolamine + H2O = 1,2-dioleoyl-sn-glycero-3-phosphate + N-palmitoyl-phosphatidylethanolamine

3.1.4.54

1-O-octadecenyl-2-oleoyl-sn-glycero-3-phospho(N-palmitoyl)ethanolamine + H2O = 1-O-octadecenyl-2-oleoyl-sn-glycerol-3-phosphate + N-palmitoyl-phosphatidylethanolamine

3.1.4.54

2 entries

Enzyme Kinetic Parameters

K_M Value (26 results)

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Show KM Value (26 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

3.1.4.4

0.0029

mutant D389N, 37°C, pH 7.4, presence of 10 mM MgCl2

680640

3.1.4.4

0.0029

mutant DELTAN55, 37°C, pH 7.4, presence of 10 mM MgCl2

680640

3.1.4.4

0.0036

mutant 387stop, 37°C, pH 7.4, presence of 10 mM MgCl2

680640

3.1.4.4

0.0037

mutant S152A, 37°C, pH 7.4, presence of 10 mM MgCl2

680640

3.1.4.4

0.0042

mutant C237S, 37°C, pH 7.4, presence of 10 mM MgCl2

680640

3.1.4.4

0.0043

mutant C222S, 37°C, pH 7.4, presence of 10 mM MgCl2

680640

3.1.4.4

0.0045

mutant C288S, 37°C, pH 7.4, presence of 10 mM MgCl2

680640

3.1.4.4

0.0046

wild-type, 37°C, pH 7.4, presence of 10 mM MgCl2

680640

3.1.4.4

0.0047

mutant C170S, 37°C, pH 7.4, presence of 10 mM MgCl2

680640

3.1.4.4

0.0047

wild-type, 37°C, pH 7.4, presence of 10 mM MgCl2

680640

3.1.4.4

0.0059

wild-type, 37°C, pH 7.4

680640

3.1.4.4

0.0061

mutant C255S, 37°C, pH 7.4, presence of 10 mM MgCl2

680640

3.1.4.4

0.008

pH 7.5, 37°C, in presence of Cacl2

650338

3.1.4.4

0.0143

mutant C224S, 37°C, pH 7.4, presence of 10 mM MgCl2

680640

3.1.4.4

0.0211

mutant H331N, 37°C, pH 7.4, presence of 10 mM MgCl2

680640

3.1.4.4

0.045

pH 7.5, 37°C, in presence of Triton X-100

650338

3.1.4.54

0.0017

pH 7.4, 37°C, 10 mM CaCl2, 0.1 mM 1,2-dioleoyl-phosphatidylethanolamine

710098

3.1.4.54

0.0022

pH 7.4, 37°C, 5 mM EDTA

710098

3.1.4.54

0.0038

pH 7.4, 37°C, 5 mM EDTA, 0.1 mM 1,2-dipalmitoyl-phosphatidylcholine

710098

3.1.4.54

0.004

pH 7.4, 37°C, 10 mM CaCl2

710098

3.1.4.54

0.0069

pH 7.4, 37°C, 10 mM CaCl2, 0.1 mM 1,2-dioleoyl-phosphatidylserine

710098

3.1.4.54

0.008

37°C, pH not specified in the publication, Ca2+ stimulated enzyme

666213

3.1.4.54

0.0116

pH 7.4, 37°C, 5 mM EDTA, 0.1 mM 1,2-dioleoyl-phosphatidylethanolamine

710098

3.1.4.54

0.0122

pH 7.4, 37°C, 10 mM CaCl2, 0.1 mM 1,2-dipalmitoyl-phosphatidylcholine

710098

3.1.4.54

0.0122

pH 7.4, 37°C, 5 mM EDTA, 0.1 mM 1,2-dioleoyl-phosphatidylserine

710098

3.1.4.54

0.045

37°C, pH not specified in the publication, Triton X-100 stimulated enzyme

References & Links

Literature References (6)

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BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

650338

11420181

Marked activation of the N-acylphosphatidylethanolamine-hydrolyzing phosphodiesterase by divalent cations

2001

Ueda, N.; Liu, Q.; Yamanaka, K.

Biochim. Biophys. Acta

1532

121-127

666213

15949819

N-acylphosphatidylethanolamine-hydrolyzing phospholipase D: a novel enzyme of the beta-lactamase fold family releasing anandamide and other N-acylethanolamines

2005

Ueda, N.; Okamoto, Y.; Morishita, J.

Life Sci.

1750-1758

680640

16527816

Functional analysis of the purified anandamide-generating phospholipase D as a member of the metallo-beta-lactamase family

2006

Wang, J.; Okamoto, Y.; Morishita, J.; Tsuboi, K.; Miyatake, A.; Ueda, N.

J. Biol. Chem.

281

12325-12335