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Ligand alpha-naphthylbutyrate

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (16 results)

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Show Substrate (16 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.1.1.1

1-naphthyl butanoate + H2O = 1-naphthol + butanoate

681338, 661545

-

3.1.1.1

1-naphthyl butyrate + H2O = 1-naphthol + butyrate

170987, 170979, 170982, 170981, 729255

-

3.1.1.1

alpha-naphthyl butanoate + H2O = alpha-naphthol + butanoate

680782

-

3.1.1.1

alpha-naphthyl butyrate + H2O = alpha-naphthol + butyrate

649399, 651506, 661545, 691913, 692622

-

3.1.1.1

4 entries

3.1.1.2

1-naphthyl butyrate + H2O = 1-naphthol + butyrate

750166

-

3.1.1.3

1-naphthyl butyrate + H2O = 1-naphthol + butyrate

730877

-

3.1.1.3

alpha-naphthyl butyrate + H2O = alpha-naphthol + butyrate

651516

-

3.1.1.3

2 entries

3.1.1.56

1-naphthyl butyrate + H2O = 1-naphthol + butyrate

646036

-

3.1.1.59

alpha-naphthyl butyrate + H2O = alpha-naphthol + butyrate

651510

-

3.1.1.6

1-naphthyl butyrate + H2O = 1-naphthol + butyrate

750541

-

3.1.1.72

1-naphthyl butanoate + H2O = 1-naphthol + butanoate

749664

-

3.1.1.73

1-naphthyl butyrate + H2O = 1-naphthol + butanoate

667790

-

3.1.1.73

1-naphthyl butyrate + H2O = 1-naphthol + butyrate

678785

-

3.1.1.73

1-naphthyl butyrate + H2O = butyric acid + 1-naphthol

208310, 708923, 699282

-

3.1.1.73

alpha-naphthyl butyrate + H2O = alpha-naphthol + butyrate

707256

-

3.1.1.73

4 entries

3.1.2.12

alpha-naphthyl butyrate + H2O = alpha-naphthol + butyrate

710495

-

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (16 results)

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Show Turnover Number (16 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

3.1.1.1

9

-

in 2.5 mM Bicine buffer, pH 8.3, at 30°C

729255

3.1.1.1

18.7

-

pH 7.0, 25°C

681338

3.1.1.1

48

-

-

170982

3.1.1.1

1600

-

-

661545

3.1.1.1

1600

-

-

661545

3.1.1.1

5 entries

3.1.1.2

9.8

-

immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C

750166

3.1.1.73

2.33

-

pH 5.0, 37°C, wild type enzyme

667790

3.1.1.73

2.7

-

pH 5.0, 37°C, D71I mutant enzyme

667790

3.1.1.73

3.38

-

pH 5.0, 37°C, Y80F mutant enzyme

667790

3.1.1.73

3.72

-

pH 5.0, 37°C, D77I mutant enzyme

667790

3.1.1.73

3.77

-

pH 5.0, 37°C, T72R mutant enzyme

667790

3.1.1.73

21

-

37°C, pH 5.0, mutant enzyme N79Q

678785

3.1.1.73

32.1

-

pH 5.0, 37°C, D77N mutant enzyme

667790

3.1.1.73

97

-

37°C, pH 5.0, mutant enzyme N79A

678785

3.1.1.73

153

-

37°C, pH 5.0, wild-type enzyme

678785

3.1.1.73

9 entries

3.1.2.12

1.73

-

in Tris-HCl buffer, pH 8.0

710495

K_M Value (17 results)

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Show KM Value (17 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

3.1.1.1

0.0076

-

-

170982

3.1.1.1

0.055

-

-

170981

3.1.1.1

0.18

-

in 2.5 mM Bicine buffer, pH 8.3, at 30°C

729255

3.1.1.1

0.33

-

-

170979

3.1.1.1

1.1

-

pH 7.0, 25°C

681338

3.1.1.1

5 entries

3.1.1.2

0.369

-

immobilized cross-linked enzyme aggregate, at pH 7.0 and 25°C

750166

3.1.1.3

0.08

-

pH 7.5, 30°C

651516

3.1.1.73

0.15

-

pH 5.0, 37°C, D77I mutant enzyme

667790

3.1.1.73

0.18

-

pH 5.0, 37°C, D77N mutant enzyme

667790

3.1.1.73

0.26

-

37°C, pH 5.0, wild-type enzyme

678785

3.1.1.73

0.28

-

37°C, pH 5.0, mutant enzyme N79Q

678785

3.1.1.73

0.29

-

pH 5.0, 37°C, D71I mutant enzyme

667790

3.1.1.73

0.33

-

pH 5.0, 37°C, wild type enzyme

667790

3.1.1.73

0.63

-

pH 5.0, 37°C, T72R mutant enzyme

667790

3.1.1.73

0.63

-

pH 5.0, 37°C, Y80F mutant enzyme

667790

3.1.1.73

0.66

-

37°C, pH 5.0, mutant enzyme N79A

678785

3.1.1.73

9 entries

3.1.2.12

0.44

-

in Tris-HCl buffer, pH 8.0

710495

References & Links

Literature References (26)

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Show Reference (26 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

170979

2246262

Human monocyte carboxylesterase. Purification and kinetics

1990

Saboori, A.M.; Newcombe, D.S.

J. Biol. Chem.

265

19792-19799

170981

-

Purification, characterization and properties of carboxylesterase from the midgut of the silkworm, Bombyx mori L

1996

Siddalinga Murthy, K.R.; Veerabhadrappa, P.S.

Insect Biochem. Mol. Biol.

26

287-296

170982

3341748

Molecular and catalytic properties of a butyrylesterase from human red cells and brain

1988

Hojring, N.; Svensmark, O.

Arch. Biochem. Biophys.

260

351-358

170987

6380369

Purification and properties of carboxylesterase B of Escherichia coli

1984

Goullet, P.; Picard, B.; Laget, P.F.

Ann. Microbiol.

135A

375-387

208310

9836439

Purification and characterization of a feruloylesterase from Aspergillus awamori

1998

Koseki, T.; Furuse, S.; Iwano, K.; Matsuzawa, H.

Biosci. Biotechnol. Biochem.

62

2032-2034

646036

-

Esterase D polymorphism and partial characterization of red blood cell esterases in cattle and buffalo populations

1996

Lama, S.N.D.; Lama, M.A.D.; Mestriner, M.A.; Mortari, N.

Braz. J. Genet.

19

243-248

649399

11152069

Cloning and characterization of EstC from Burkholderia gladioli, a novel-type esterase related to plant enzymes

2000

Reiter, B.; Glieder, A.; Talker, D.; Schwab, H.

Appl. Microbiol. Biotechnol.

54

778-785

651506

11267909

Purification and characterization of trans-permethrin metabolizing microsomal esterases from workers of the eastern subterranean termite, Reticulitermes flavipes (Kollar)

2001

Valles, S.M.; Oi, F.M.; Strong, C.A.

Insect Biochem. Mol. Biol.

31

715-725

651510

14599498

Juvenile hormone (JH) esterase: why are you so JH specific?

2003

Kamita, S.G.; Hinton, A.C.; Wheelock, C.E.; Wogulis, M.D.; Wilson, D.K.; Wolf, N.M.; Stok, J.E.; Hock, B.; Hammock, B.D.

Insect Biochem. Mol. Biol.

33

1261-1273

651516

100138638

Purification and characterization of an extracellular tributyrin esterase produced by a cheese isolate, Micrococcus sp. INIA 528

2004

Fernandez, J.; Mohedano, A.F.; Fernandez-Garcia, E.; Medina, M.; Nunez, M.

Int. Dairy J.

14

135-142

661545

15717224

Isolation, characterization, and heterologous expression of a carboxylesterase of Pseudomonas aeruginosa PAO1

2005

Pesaresi, A.; Devescovi, G.; Lamba, D.; Venturi, V.; Degrassi, G.

Curr. Microbiol.

50

102-109

667790

15716038

Mutational analysis of a feruloyl esterase from Aspergillus awamori involved in substrate discrimination and pH dependence

2005

Koseki, T.; Takahashi, K.; Fushinobu, S.; Iefuji, H.; Iwano, K.; Hashizume, K.; Matsuzawa, H.

Biochim. Biophys. Acta

1722

200-208

678785

17031032

N-linked oligosaccharides of Aspergillus awamori feruloyl esterase are important for thermostability and catalysis

2006

Koseki, T.; Takahashi, K.; Handa, T.; Yamane, Y.; Fushinobu, S.; Hashizume, K.

Biosci. Biotechnol. Biochem.

70

2476-2480

680782

17428787

Characterization of a novel cell wall-anchored protein with carboxylesterase activity required for virulence in Mycobacterium tuberculosis

2007

Lun, S.; Bishai, W.R.

J. Biol. Chem.

282

18348-18356

681338

-

Expression and characterization of a new esterase cloned directly from Agrobacterium tumefaciens genome

2006

Park, H.; Kim, Y.; Kim, H.

J. Microbiol. Biotechnol.

16

145-148

691913

19110065

Carboxylesterase activity, cDNA sequence, and gene expression in malathion susceptible and resistant strains of the cotton aphid, Aphis gossypii

2009

Pan, Y.; Guo, H.; Gao, X.

Comp. Biochem. Physiol. B

152

266-270

692622

-

Differential mRNA expression levels and gene sequences of carboxylesterase in both deltamethrin resistant and susceptible strains of the cotton aphid, Aphis gossypii

2008

Cao, C.; Zhang, J.; Gao, X.; Liang, P.; Guo, H.

Insect Sci.

15

209-216

699282

19241534

Purification and characteristics of feruloyl esterase from Aspergillus awamori G-2 strain

2008

Kancuchi, M.; Watanabe, S.; Tsukada, T.; Atta, K.; Kakuta, T.; Koizumi, T.

J. Food Sci.

73

C458-C463

707256

19756576

Characterization of a chimeric enzyme comprising feruloyl esterase and family 42 carbohydrate-binding module

2010

Koseki, T.; Mochizuki, K.; Kisara, H.; Miyanaga, A.; Fushinobu, S.; Murayama, T.; Shiono, Y.

Appl. Microbiol. Biotechnol.

86

155-161

708923

19304844

Biochemical analysis of a beta-D-xylosidase and a bifunctional xylanase-ferulic acid esterase from a xylanolytic gene cluster in Prevotella ruminicola 23

2009

Dodd, D.; Kocherginskaya, S.A.; Spies, M.A.; Beery, K.E.; Abbas, C.A.; Mackie, R.I.; Cann, I.K.

J. Bacteriol.

191

3328-3338

710495

19653299

The structure of a putative S-formylglutathione hydrolase from Agrobacterium tumefaciens

2009

van Straaten, K.E.; Gonzalez, C.F.; Valladares, R.B.; Xu, X.; Savchenko, A.V.; Sanders, D.A.

Protein Sci.

18

2196-2202

729255

24832101

Structural characterization and function determination of a nonspecific carboxylate esterase from the amidohydrolase superfamily with a promiscuous ability to hydrolyze methylphosphonate esters

2014

Xiang, D.F.; Kumaran, D.; Swaminathan, S.; Raushel, F.M.

Biochemistry

53

3476-3485

730877

23202292

Rice (Oryza sativa) lipase: molecular cloning, functional expression and substrate specificity

2013

Vijayakumar, K.R.; Gowda, L.R.

Protein Expr. Purif.

88

67-79

749664

28802264

Crystal structure and substrate specificity modification of acetyl xylan esterase from Aspergillus luchuensis

2017

Komiya, D.; Hori, A.; Ishida, T.; Igarashi, K.; Samejima, M.; Koseki, T.; Fushinobu, S.

Appl. Environ. Microbiol.

83

e01251

750166

26443058

Characterization of cross-linked immobilized arylesterase from Gluconobacter oxydans 621H with activity toward cephalosporin C and 7-aminocephalosporanic acid

2016

Inmaculada, N.; Francisco, G.

Biotechnol. Prog.

32

36-42

750541

26991446

Structural and biochemical characterization of an octameric carbohydrate acetylesterase from Sinorhizobium meliloti

2016

Oh, C.; Ryu, B.H.; An, D.R.; Nguyen, D.D.; Yoo, W.; Kim, T.; Ngo, T.D.; Kim, H.S.; Kim, K.K.; Kim, T.D.

FEBS Lett.

590

1242-1252

Links to other databases for alpha-naphthylbutyrate

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Show Links (1 entry)

ChEBI

PubChem

-