Ligand resorufin

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Basic Ligand Information

Molecular Structure
Picture of resorufin (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C12H7NO3
resorufin
HSSLDCABUXLXKM-UHFFFAOYSA-N
Synonyms:
7-hydroxy-3H-phenoxazin-3-one, 7-hydroxyresorufin, resolufin

Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (3 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
resorufin + NADH = ? + NAD+
show the reaction diagram
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resorufin + beta-D-glucose + H2O = resorufin-beta-D-glucoside
show the reaction diagram
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ATP + H2O + resorufine/in = ADP + phosphate + resorufine/out
show the reaction diagram
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Product in Enzyme-catalyzed Reactions (10 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
trans,octacis-decaprenylphospho-beta-D-ribofuranose + resazurin + FAD = trans,octacis-decaprenylphospho-beta-D-erythro-pentofuranosid-2-ulose + resorufin + FADH2
show the reaction diagram
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10-acetyl-3,7-dihydroxyphenoxazine + H2O2 + H+ = resorufin + H2O
show the reaction diagram
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7-ethoxyresorufin + [reduced NADPH-hemoprotein reductase] + O2 = resorufin + ethanol + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
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Amplex red + H2O = resorufin + acetate
show the reaction diagram
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7-hydroxyresorufinyl arachidonate + H2O = 7-hydroxyresorufin + arachidonate
show the reaction diagram
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resorufin 3-O-beta-D-glucoside + H2O = D-glucose + resorufin
show the reaction diagram
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7-O-beta-D-galactosylresorufin + H2O = resorufin + beta-D-galactose
show the reaction diagram
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resolufin-labeled casein + H2O = resolufin + casein
show the reaction diagram
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amplex red + NADH + O2 = resorufin + NAD+ + H2O2
show the reaction diagram
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ATP + H2O + resorufine/in = ADP + phosphate + resorufine/out
show the reaction diagram
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3D Structure of Enzyme-Ligand-Complex (PDB) (1 result)

EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (1 result)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
21.9
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isoform EGCrP2, at pH 6.0 and 37°C

KM Value (1 result)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.0436
-
isoform EGCrP2, at pH 6.0 and 37°C

References & Links

Links to other databases for resorufin

ChEBI
PubChem
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PubChem