Ligand ppGpp

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Basic Ligand Information

Molecular Structure
Picture of ppGpp (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C10H17N5O17P4
ppGpp
BUFLLCUFNHESEH-UUOKFMHZSA-N
Synonyms:
Guanosine-3',5'-bis(diphosphate), guanosine-5',3'-dibisphosphate, Guanosine-5'-diphosphate-3'-diphosphate, guanosine 3',5'-bis(diphosphate), guanosine 3',5'-bis(trihydrogen diphosphate), guanosine 3',5'-bis-diphosphate, guanosine 3',5'-bisdiphosphate, guanosine 3'-diphosphate 5'-diphosphate, Guanosine 3,5'-bis(diphosphate), Guanosine 5'-diphosphate,3'-diphosphate, Guanosine 5'-diphosphate-3'-diphosphate, guanosine 5'-diphosphate 3'-diphosphate


Show all pahtways known for Show all BRENDA pathways known for ppGpp

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (3 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
guanosine 3'-diphosphate 5'-diphosphate + H2O = GDP + diphosphate
show the reaction diagram

In Vivo Product in Enzyme-catalyzed Reactions (3 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
ATP + GDP = AMP + guanosine 3'-diphosphate 5'-diphosphate
show the reaction diagram
-

Substrate in Enzyme-catalyzed Reactions (4 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
guanosine 3'-diphosphate 5'-diphosphate + H2O = GDP + diphosphate
show the reaction diagram
guanosine 3',5'-bis(diphosphate) + 2 H2O = guanosine 3',5'-bisphoshate + 2 phosphate
show the reaction diagram
-

Product in Enzyme-catalyzed Reactions (7 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE

Activator in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
ppGpp, potential allosteric regulator, activates the enzyme with an EC50 of 0.060 mM

Inhibitor in Enzyme-catalyzed Reactions (14 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
in presence of partially inhibiting concentrations of histidine guanosine 5'-diphosphate-3'-diphosphate becomes a potent inhibitor of the residual activity of ATP phosphoribosyltransferase, no inhibition in absence of histidine, inhibition is slowly reversible
-
competitive inhibitor
inhibitory, impedes primase activity by blocking entry of an incoming NTP, and interfering with the binding of either an initiating 5'-NTP, or the extensible 3'-end of an RNA-DNA heteroduplex
-

3D Structure of Enzyme-Ligand-Complex (PDB) (149 results)

EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (3 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.49
-
pH 8.0, 37C, presence of 5 mM Mg2+

KM Value (3 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
3
-
pH 7.5, 37C, recombinant enzyme
0.41
-
pH 8.0, 30C, KM is increased 3fold in the presence of the complex containing tRNA, ribosomes, and mRNA
0.135
-
pH 8.0, 37C, presence of 5 mM Mg2+

Ki Value (9 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.0028
-
versus GMP, pH 7.5, 30C
0.25
-
30C

IC50 Value (2 results)

EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE
0.0033
-
at pH 7.6 and 25C
0.01
-
versus GMP, pH 7.5, 30C

References & Links

Links to other databases for ppGpp

ChEBI
PubChem
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PubChem