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Ligand Pyrazinamide

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (4 results)

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Show Natural Substrate (4 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.5.1.19

pyrazinamide + H2O = NH3 + pyrazinoic acid

677824, 679806

-

3.5.1.19

pyrazinamide + H2O = pyrazine-2-carboxylic acid + NH3

720499, 718885, 718859

-

3.5.1.19

pyrazinamide + H2O = pyrazinoic acid + NH3

710862, 735084, 735054, 735324, 773014

-

3.5.1.19

3 entries

3.5.1.B15

pyrazinamide + H2O = pyrazinoic acid + NH3

710862, 735054, 735324, 757448, 758283, 758499, 756295, 753679, 757008, 757293, 757291, 756516, 757007, 757258, 755800, 752683, 756937, 757305, 757425, 757445

-

Substrate in Enzyme-catalyzed Reactions (7 results)

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Show Substrate (7 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.5.1.19

pyrazinamide + H2O = NH3 + pyrazinoic acid

677824, 679806

-

3.5.1.19

pyrazinamide + H2O = pyrazine-2-carboxylic acid + NH3

720499, 718885, 718859, 718924

-

3.5.1.19

pyrazinamide + H2O = pyrazinoic acid + NH3

246582, 246584, 246585, 246588, 246583, 698815, 710862, 735084, 735054, 735324, 734741, 755150, 753679, 773014

-

3.5.1.4

pyrazinamide + H2O = ? + NH3

667278

-

3.5.1.4

pyrazinamide + H2O = pyrazinate + NH3

718672, 733190

-

3.5.1.4

pyrazinamide + H2O = pyrazinoic acid + NH3

719432

-

3.5.1.19

3 entries

3.5.1.4

3 entries

3.5.1.B15

pyrazinamide + H2O = pyrazinoic acid + NH3

710862, 727053, 735054, 735324, 744296, 757448, 758283, 758499, 756295, 753679, 757008, 757293, 757291, 756516, 757007, 757258, 755800, 752683, 756937, 757305, 757425, 757445, 772685

-

Product in Enzyme-catalyzed Reactions (2 results)

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Show Product (2 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

4.2.1.84

2-cyanopyrazine + H2O = pyrazincarbamide

-

-

4.2.1.84

cyanopyrazine + H2O = pyrazincarbamide

-

-

4.2.1.84

2 entries

Inhibitor in Enzyme-catalyzed Reactions (10 results)

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Show Inhibitors (10 entries)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

1.3.1.9

-

764624

-

2.1.1.7

-

441221

-

2.3.1.286

1% inhibition at 5 mM

749878

-

2.3.1.286

4% inhibition at 5 mM

749878

-

2.3.1.286

2 entries

2.3.1.37

about 85% residual activity at 0.01 mM

756091

-

2.3.1.85

IC50: 8.9 mM

671378

-

2.4.2.19

-

737126

-

2.4.2.30

IC50: 0.13 mM

638001

-

3.5.1.19

moderate

246574

-

3.5.1.19

the natural resistance of Mycobacterium kansasii to pyrazinamide is due to a deficient pyrazinamidase activity of nicotinamidase

246583

-

3.5.1.19

2 entries

3D Structure of Enzyme-Ligand-Complex (PDB) (5 results)

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Show 3D Structure of Enzyme-Ligand-Complex (PDB) (5 entries)

EC NUMBER

ENZYME 3D STRUCTURE

1.11.1.7

3R4X, 3D-view

1.11.1.7

3R55, 3D-view

1.11.1.7

3R4X, 3D-view

1.11.1.7

3R55, 3D-view

1.11.1.7

4 entries

3.1.3.84

7H8M, 3D-view

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (22 results)

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Show Turnover Number (22 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

3.5.1.19

0.1

-

mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C

718885

3.5.1.19

0.43

-

recombinant mutant E65H, pH 7.3, 37°C

735084

3.5.1.19

0.45

-

recombinant mutant Q96K, pH 7.3, 37°C

735084

3.5.1.19

1.3

-

37°C, pH 7.3, mutant enzyme H75S

755150

3.5.1.19

1.61

-

recombinant mutant Q96A, pH 7.3, 37°C

735084

3.5.1.19

2.56

-

wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C

718924

3.5.1.19

2.6

-

recombinant wild-type enzyme, pH 7.3, 37°C

735084

3.5.1.19

3.1

-

37°C, pH 7.3, mutant enzyme W83F

755150

3.5.1.19

3.31

-

recombinant mutant C133A, pH 7.3, 37°C

735084

3.5.1.19

3.5

-

37°C, pH 7.3, mutant enzyme H75E

755150

3.5.1.19

3.8

-

wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C

718885

3.5.1.19

3.94

-

recombinant mutant F68W, pH 7.3, 37°C

735084

3.5.1.19

5.21

-

recombinant mutant F68W/C133A, pH 7.3, 37°C

735084

3.5.1.19

7.2

-

pH 7.3, temperature not specified in the publication

753679

3.5.1.19

7.5

-

37°C, pH 7.3, mutant enzyme A151C

755150

3.5.1.19

18.7

-

37°C, pH 7.3, wild-type enzyme

755150

3.5.1.B15

0.725

-

pH 6.5, 37°C, recombinant His-tagged mutant L151S

758283

3.5.1.B15

1.08

-

pH 6.5, 37°C, recombinant His-tagged mutant A143T

758283

3.5.1.B15

1.67

-

pH 6.5, 37°C, recombinant His-tagged mutant A143T/T168A/E173K

758283

3.5.1.B15

2

-

pH 6.5, 37°C, recombinant His-tagged wild-type enzyme

758283

3.5.1.B15

14.55

-

pH 6.5, temperature not specified in the publication

772685

3.5.1.B15

135.7

-

pH 7.5, 60°C

727053

3.5.1.19

16 entries

3.5.1.B15

6 entries

K_M Value (24 results)

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Show KM Value (24 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

3.5.1.19

0.016

-

mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C

718885

3.5.1.19

0.056

-

-

246588

3.5.1.19

0.103

-

pH 7.3, temperature not specified in the publication

753679

3.5.1.19

0.157

-

wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C

718924

3.5.1.19

0.22

-

37°C, pH 7.3, mutant enzyme A151C

755150

3.5.1.19

0.3

-

-

246585

3.5.1.19

0.3

-

wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C

718885

3.5.1.19

0.33

-

37°C, pH 7.3, wild-type enzyme

755150

3.5.1.19

0.36

-

recombinant mutant C133A, pH 7.3, 37°C

735084

3.5.1.19

0.5

-

recombinant mutant F68W, pH 7.3, 37°C

735084

3.5.1.19

0.59

-

37°C, pH 7.3, mutant enzyme H75S

755150

3.5.1.19

0.67

-

37°C, pH 7.3, mutant enzyme H75E

755150

3.5.1.19

0.81

-

recombinant wild-type enzyme, pH 7.3, 37°C

735084

3.5.1.19

0.82

-

recombinant mutant E65H, pH 7.3, 37°C

735084

3.5.1.19

0.83

-

recombinant mutant F68W/C133A, pH 7.3, 37°C

735084

3.5.1.19

1.15

-

recombinant mutant Q96K, pH 7.3, 37°C

735084

3.5.1.19

1.2

-

recombinant mutant Q96A, pH 7.3, 37°C

735084

3.5.1.19

2.89

-

37°C, pH 7.3, mutant enzyme W83F

755150

3.5.1.B15

0.36

-

pH 6.5, temperature not specified in the publication

772685

3.5.1.B15

0.41

-

pH 7.5, 60°C

727053

3.5.1.B15

0.431

-

pH 6.5, 37°C, recombinant His-tagged mutant L151S

758283

3.5.1.B15

0.46

-

pH 6.5, 37°C, recombinant His-tagged mutant A143T

758283

3.5.1.B15

0.68

-

pH 6.5, 37°C, recombinant His-tagged mutant A143T/T168A/E173K

758283

3.5.1.B15

0.79

-

pH 6.5, 37°C, recombinant His-tagged wild-type enzyme

758283

3.5.1.19

18 entries

3.5.1.B15

6 entries

K_i Value (1 result)

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Show KI Value (1 entry)

EC NUMBER

KI VALUE [MM]

KI VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.3.1.85

2.6

-

37°C, pH 7.4

671378

IC₅₀ Value (4 results)

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Show IC50 Value (4 entries)

EC NUMBER

IC50 VALUE

IC50 VALUE MAXIMUM

COMMENTARY

LITERATURE

2.3.1.85

8.9

-

IC50: 8.9 mM

671378

2.4.2.19

0.38

-

at pH 7.2 and 37°C

737126

2.4.2.19

1.37

-

at pH 6.2 and 37°C

737126

2.4.2.19

2 entries

2.4.2.30

0.13

-

IC50: 0.13 mM

638001

References & Links

Literature References (71)

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Show Reference (71 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

246574

-

Inhibition of nicotinamide deaminase from Micrococcus lysodeikticus by analogues of nicotinamide

1974

Johnson, W.J.; Gadd, R.E.A.

Int. J. Biochem.

5

633-641

246582

8726014

Identification, cloning, and expression of the Escherichia coli pyrazinamidase and nicotinamidase gene, pncA

1996

Frothingham, R.; Meeker-OæConnell, W.A.; Talbot, E.A.S.; George, J.W.; Kreuzer K.N.

Antimicrob. Agents Chemother.

40

1426-1431

246583

10049264

Reduced pyrazinamidase activity and the natural resistance of Mycobacterium kansasii to the antituberculois drug pyrazinamide

1999

Sun, Z.; Zhang, Y.

Antimicrob. Agents Chemother.

43

537-542

246584

10851013

Mycobacterium smegmatis has two pyrazinamidase enzymes PncA and PzaA

2000

Guo, M.; Sun, Z.; Zhang, Y.

J. Bacteriol.

182

3881-3884

246585

9811635

Purification, gene cloning, targeted knockout, overexpression, and biochemical characterization of the major pyrazinamidase from Mycobacterium smegmatis

1998

Boshoff, H.I.; Mizrahi, V.

J. Bacteriol.

180

5809-5814

246588

-

Purification and properties of an aromatic amidase from Pseudomonas sp. GDI 211

1990

Kgayama, T.; Ohe, T.

Agric. Biol. Chem.

54

2565-2571

441221

-

Biosynthesis and metabolism of trigonelline in Lemna paucicostata 151

1989

Taguchi, H.; Nishitani, H.; Okumura, K.; Shimabayashi, Y.; Iwai, K.

Agric. Biol. Chem.

53

2867-2871

638001

2538435

Quantitative studies of inhibitors of ADP-ribosylation in vitro and in vivo

1989

Rankin, P.W.; Jacobson, E.L.; benjamin, R.C.; Moss, J.; Jacobson, M.K.

J. Biol. Chem.

264

4312-4317

667278

15103424

Purification and properties of an enantioselective and thermoactive amidase from the thermophilic actinomycete Pseudonocardia thermophila

2004

Egorova, K.; Trauthwein, H.; Verseck, S.; Antranikian, G.

Appl. Microbiol. Biotechnol.

65

38-45

671378

17485499

Inhibition of isolated Mycobacterium tuberculosis fatty acid synthase I by pyrazinamide analogs

2007

Ngo, S.C.; Zimhony, O.; Chung, W.J.; Sayahi, H.; Jacobs, W.R.; Welch, J.T.

Antimicrob. Agents Chemother.

51

2430-2435

677824

17382284

Crystal structure of the yeast nicotinamidase Pnc1p

2007

Hu, G.; Taylor, A.B.; McAlister-Henn, L.; Hart, P.J.

Arch. Biochem. Biophys.

461

66-75

679806

18201201

Characterization of Mycobacterium tuberculosis nicotinamidase/pyrazinamidase

2008

Zhang, H.; Deng, J.Y.; Bi, L.J.; Zhou, Y.F.; Zhang, Z.P.; Zhang, C.G.; Zhang, Y.; Zhang, X.E.

FEBS J.

275

753-762

698815

18678867

Life span extension and neuronal cell protection by Drosophila nicotinamidase

2008

Balan, V.; Miller, G.S.; Kaplun, L.; Balan, K.; Chong, Z.Z.; Li, F.; Kaplun, A.; VanBerkum, M.F.; Arking, R.; Freeman, D.C.; Maiese, K.; Tzivion, G.

J. Biol. Chem.

283

27810-27819

710862

19859929

Specificity and mechanism of Acinetobacter baumanii nicotinamidase: Implications for activation of the front-line tuberculosis drug pyrazinamide

2009

Fyfe, P.; Rao, V.; Zemla, A.; Cameron, S.; Hunter, W.

Angew. Chem. Int. Ed. Engl.

48

9176-9179

718672

22101784

Cloning and characterization of a novel amidase from Paracoccus sp. M-1, showing aryl acylamidase and acyl transferase activities

2012

Shen, W.; Chen, H.; Jia, K.; Ni, J.; Yan, X.; Li, S.

Appl. Microbiol. Biotechnol.

94

1007-1018

718859

20979384

Characterization of nicotinamidases: steady state kinetic parameters, classwide inhibition by nicotinaldehydes, and catalytic mechanism

2010

French, J.B.; Cen, Y.; Vrablik, T.L.; Xu, P.; Allen, E.; Hanna-Rose, W.; Sauve, A.A.

Biochemistry

49

10421-10439

718885

20879713

Kinetics and inhibition of nicotinamidase from Mycobacterium tuberculosis

2010

Seiner, D.R.; Hegde, S.S.; Blanchard, J.S.

Biochemistry

49

9613-9619

718924

22229411

Structural and kinetic isotope effect studies of nicotinamidase (Pnc1) from Saccharomyces cerevisiae

2012

Smith, B.C.; Anderson, M.A.; Hoadley, K.A.; Keck, J.L.; Cleland, W.W.; Denu, J.M.

Biochemistry

51

243-256

719432

11453462

Molecular and biochemical characterization of the recombinant amidase from hyperthermophilic archaeon Sulfolobus solfataricus

2001

dAbusco, A.S.; Ammendola, S.; Scandurra, R.; Politi, L.

Extremophiles

5

183-192

720499

21819459

The Leishmania nicotinamidase is essential for NAD+ production and parasite proliferation

2011

Gazanion, E.; Garcia, D.; Silvestre, R.; Gerard, C.; Guichou, J.F.; Labesse, G.; Seveno, M.; Cordeiro-Da-Silva, A.; Ouaissi, A.; Sereno, D.; Vergnes, B.

Mol. Microbiol.

82

21-38

727053

24512664

Nicotinamidase from the thermophilic archaeon Acidilobus saccharovorans: structural and functional characteristics

2014

Stekhanova, T.N.; Bezsudnova, E.Y.; Mardanov, A.V.; Osipov, E.M.; Ravin, N.V.; Skryabin, K.G.; Popov, V.O.

Biochemistry

79

54-61

727053

24512664

Nicotinamidase from the thermophilic archaeon Acidilobus saccharovorans: structural and functional characteristics

2014

Stekhanova, T.N.; Bezsudnova, E.Y.; Mardanov, A.V.; Osipov, E.M.; Ravin, N.V.; Skryabin, K.G.; Popov, V.O.

Biochemistry (Moscow)

79

54-61

727053

2 entries

733190

24752846

Nitrilase superfamily aryl acylamidase from the halotolerant mangrove Streptomyces sp. 211726

2014

Ma, Y.; Xu, W.; Zhang, J.; Zhang, S.; Hong, K.; Deng, Z.; Sun, Y.

Appl. Microbiol. Biotechnol.

98

8583-8590

734741

25268724

A multi-scale computational study on the mechanism of Streptococcus pneumoniae nicotinamidase (SpNic)

2014

Ion, B.F.; Kazim, E.; Gauld, J.W.

Molecules

19

15735-15753

735054

22761821

Molecular dynamics simulations suggest ligands binding to nicotinamidase/pyrazinamidase

2012

Zhang, J.L.; Zheng, Q.C.; Li, Z.Q.; Zhang, H.X.

PLoS ONE

7

e39546

735084

23451075

Biochemical and mutational analysis of a novel nicotinamidase from Oceanobacillus iheyensis HTE831

2013

Sanchez-Carron, G.; Garcia-Garcia, M.I.; Zapata-Perez, R.; Takami, H.; Garcia-Carmona, F.; Sanchez-Ferrer, A.

PLoS ONE

8

e56727

735324

25199451

Nicotinamidase/pyrazinamidase of Mycobacterium tuberculosis forms homo-dimers stabilized by disulfide bonds

2014

Rueda, D.; Sheen, P.; Gilman, R.H.; Bueno, C.; Santos, M.; Pando-Robles, V.; Batista, C.V.; Zimic, M.

Tuberculosis

94

644-648

737126

24949952

Biochemical characterization of quinolinic acid phosphoribosyltransferase from Mycobacterium tuberculosis H37Rv and inhibition of its activity by pyrazinamide

2014

Kim, H.; Shibayama, K.; Rimbara, E.; Mori, S.

PLoS ONE

9

e100062

744296

11714269

Crystal structure and mechanism of catalysis of a pyrazinamidase from Pyrococcus horikoshii

2001

Du, X.; Wang, W.; Kim, R.; Yakota, H.; Nguyen, H.; Kim, S.H.

Biochemistry

40

14166-14172

744296

11714269

Crystal structure and mechanism of catalysis of a pyrazinamidase from Pyrococcus horikoshii

2001

Du, X.; Wang, W.; Kim, R.; Yakota, H.; Nguyen, H.; Kim, S.H.

Biochemistry

40

14166-14172

744296

2 entries

749878

12899610

Sir2 regulation by nicotinamide results from switching between base exchange and deacetylation chemistry

2003

Sauve, A.A.; Schramm, V.L.

Biochemistry

42

9249-9256

752683

30107912

Crystal structure of the nicotinamidase/pyrazinamidase PncA from Bacillus subtilis

2018

Shang, F.; Chen, J.; Wang, L.; Jin, L.; Zou, L.; Bu, T.; Dong, Y.; Ha, N.C.; Nam, K.H.; Quan, C.; Xu, Y.

Biochem. Biophys. Res. Commun.

503

2906-2911

752683

30107912

Crystal structure of the nicotinamidase/pyrazinamidase PncA from Bacillus subtilis

2018

Shang, F.; Chen, J.; Wang, L.; Jin, L.; Zou, L.; Bu, T.; Dong, Y.; Ha, N.C.; Nam, K.H.; Quan, C.; Xu, Y.

Biochem. Biophys. Res. Commun.

503

2906-2911

753679

28018295

Combined whole-cell high-throughput functional screening for identification of new nicotinamidases/pyrazinamidases in metagenomic/polygenomic libraries

2016

Zapata-Perez, R.; Garcia-Saura, A.G.; Jebbar, M.; Golyshin, P.N.; Sanchez-Ferrer, A.

Front. Microbiol.

7

1915

753679

28018295

Combined whole-cell high-throughput functional screening for identification of new nicotinamidases/pyrazinamidases in metagenomic/polygenomic libraries

2016

Zapata-Perez, R.; Garcia-Saura, A.G.; Jebbar, M.; Golyshin, P.N.; Sanchez-Ferrer, A.

Front. Microbiol.

7

1915

755150

28750065

Biochemical characterization of a new nicotinamidase from an unclassified bacterium thriving in a geothermal water stream microbial mat community

2017

Zapata-Perez, R.; Martinez-Monino, A.B.; Garcia-Saura, A.G.; Cabanes, J.; Takami, H.; Sanchez-Ferrer, A.

PLoS ONE

12

e0181561

755150

28750065

Biochemical characterization of a new nicotinamidase from an unclassified bacterium thriving in a geothermal water stream microbial mat community

2017

Zapata-Perez, R.; Martinez-Monino, A.B.; Garcia-Saura, A.G.; Cabanes, J.; Takami, H.; Sanchez-Ferrer, A.

PLoS ONE

12

e0181561

755800

26077261

Systematic review of mutations in pyrazinamidase associated with pyrazinamide resistance in Mycobacterium tuberculosis clinical isolates

2015

Ramirez-Busby, S.M.; Valafar, F.

Antimicrob. Agents Chemother.

59

5267-5277

755800

26077261

Systematic review of mutations in pyrazinamidase associated with pyrazinamide resistance in Mycobacterium tuberculosis clinical isolates

2015

Ramirez-Busby, S.M.; Valafar, F.

Antimicrob. Agents Chemother.

59

5267-5277

756091

27838491

Isoniazid inhibits human erythroid 5-aminolevulinate synthase Molecular mechanism and tolerance study with four X-linked protoporphyria patients

2017

Fratz-Berilla, E.J.; Breydo, L.; Gouya, L.; Puy, H.; Uversky, V.N.; Ferreira, G.C.

Biochim. Biophys. Acta

1863

428-439

756091

27838491

Isoniazid inhibits human erythroid 5-aminolevulinate synthase Molecular mechanism and tolerance study with four X-linked protoporphyria patients

2017

Fratz-Berilla, E.J.; Breydo, L.; Gouya, L.; Puy, H.; Uversky, V.N.; Ferreira, G.C.

Biochim. Biophys. Acta

1863

428-439

756295

25708048

Mechanistic analysis elucidating the relationship between Lys96 mutation in Mycobacterium tuberculosis pyrazinamidase enzyme and pyrazinamide susceptibility

2015

Vats, C.; Dhanjal, J.; Goyal, S.; Gupta, A.; Bharadvaja, N.; Grover, A.

BMC Genomics

16(Suppl 2)

S14

756295

25708048

Mechanistic analysis elucidating the relationship between Lys96 mutation in Mycobacterium tuberculosis pyrazinamidase enzyme and pyrazinamide susceptibility

2015

Vats, C.; Dhanjal, J.; Goyal, S.; Gupta, A.; Bharadvaja, N.; Grover, A.

BMC Genomics

16(Suppl 2)

S14

756516

31442707

Revelation of enzyme activity of mutant pyrazinamidases from Mycobacterium tuberculosis upon binding with various metals using quantum mechanical approach

2019

Rasool, N.; Husssain, W.; Khan, Y.D.

Comput. Biol. Chem.

83

107108

756516

31442707

Revelation of enzyme activity of mutant pyrazinamidases from Mycobacterium tuberculosis upon binding with various metals using quantum mechanical approach

2019

Rasool, N.; Husssain, W.; Khan, Y.D.

Comput. Biol. Chem.

83

107108

756937

29129628

The combinatorial effects of osmolytes and alcohols on the stability of pyrazinamidase methanol affects the enzyme stability through hydrophobic interactions and hydrogen bonds

2018

Pazhang, M.; Mardi, N.; Mehrnejad, F.; Chaparzadeh, N.

Int. J. Biol. Macromol.

108

1339-1347

756937

29129628

The combinatorial effects of osmolytes and alcohols on the stability of pyrazinamidase methanol affects the enzyme stability through hydrophobic interactions and hydrogen bonds

2018

Pazhang, M.; Mardi, N.; Mehrnejad, F.; Chaparzadeh, N.

Int. J. Biol. Macromol.

108

1339-1347

757007

28043510

Effects of sorbitol and glycerol on the structure, dynamics, and stability of Mycobacterium tuberculosis pyrazinamidase

2016

Khajehzadeh, M.; Mehrnejad, F.; Pazhang, M.; Doustdar, F.

Int. J. Mycobacteriol.

5 Suppl 1

S138-S139

757007

28043510

Effects of sorbitol and glycerol on the structure, dynamics, and stability of Mycobacterium tuberculosis pyrazinamidase

2016

Khajehzadeh, M.; Mehrnejad, F.; Pazhang, M.; Doustdar, F.

Int. J. Mycobacteriol.

5 Suppl 1

S138-S139

757008

29516881

Estimation of pyrazinamidase activity using a cell-free in vitro synthesis of pnca and its association with pyrazinamide susceptibility in Mycobacterium tuberculosis

2018

Rueda, D.; Bernard, C.; Gandy, L.; Capton, E.; Boudjelloul, R.; Brossier, F.; Veziris, N.; Zimic, M.; Sougakoff, W.

Int. J. Mycobacteriol.

7

16-25

757008

29516881

Estimation of pyrazinamidase activity using a cell-free in vitro synthesis of pnca and its association with pyrazinamide susceptibility in Mycobacterium tuberculosis

2018

Rueda, D.; Bernard, C.; Gandy, L.; Capton, E.; Boudjelloul, R.; Brossier, F.; Veziris, N.; Zimic, M.; Sougakoff, W.

Int. J. Mycobacteriol.

7

16-25

757258

31357912

Structural dynamics behind variants in pyrazinamidase and pyrazinamide resistance

2020

Khan, M.T.; Malik, S.I.

J. Biomol. Struct. Dyn.

38

3003-3017

757258

31357912

Structural dynamics behind variants in pyrazinamidase and pyrazinamide resistance

2020

Khan, M.T.; Malik, S.I.

J. Biomol. Struct. Dyn.

38

3003-3017

752683

2 entries

753679

2 entries

755150

2 entries

755800

2 entries

756091

2 entries

756295

2 entries

756516

2 entries

756937

2 entries

757007

2 entries

757008

2 entries

757258

2 entries

757291

30304542

Computational insights into pH-dependence of structure and dynamics of pyrazinamidase A comparison of wild type and mutants

2018

Esmaeeli, R.; Mehrnejad, F.; Mir-Derikvand, M.; Gopalpoor, N.

J. Cell. Biochem.

120

2502-2514

757293

30485476

Pyrazinamide resistance and mutations L19R, R140H, and E144K in pyrazinamidase of Mycobacterium tuberculosis

2019

Khan, M.; Junaid, M.; Mao, X.; Wang, Y.; Hussain, A.; Malik, S.; Wei, D.

J. Cell. Biochem.

120

7154-7166

757293

30485476

Pyrazinamide resistance and mutations L19R, R140H, and E144K in pyrazinamidase of Mycobacterium tuberculosis

2019

Khan, M.; Junaid, M.; Mao, X.; Wang, Y.; Hussain, A.; Malik, S.; Wei, D.

J. Cell. Biochem.

120

7154-7166

757305

30481017

Insights into the mechanisms of the pyrazinamide resistance of three pyrazinamidase mutants N11K, P69T, and D126N

2019

Junaid, M.; Khan, M.T.; Malik, S.I.; Wei, D.Q.

J. Chem. Inf. Model.

59

498-508

757305

30481017

Insights into the mechanisms of the pyrazinamide resistance of three pyrazinamidase mutants N11K, P69T, and D126N

2019

Junaid, M.; Khan, M.T.; Malik, S.I.; Wei, D.Q.

J. Chem. Inf. Model.

59

498-508

757425

30316980

A simplified pyrazinamidase test for pyrazinamide drug susceptibility in Mycobacterium tuberculosis

2018

Aono, A.; Chikamatsu, K.; Yamada, H.; Igarashi, Y.; Murase, Y.; Takaki, A.; Mitarai, S.

J. Microbiol. Methods

154

52-54

757425

30316980

A simplified pyrazinamidase test for pyrazinamide drug susceptibility in Mycobacterium tuberculosis

2018

Aono, A.; Chikamatsu, K.; Yamada, H.; Igarashi, Y.; Murase, Y.; Takaki, A.; Mitarai, S.

J. Microbiol. Methods

154

52-54

757293

2 entries

757305

2 entries

757425

2 entries

757445

28214629

Effects of metal-ion replacement on pyrazinamidase activity a quantum mechanical study

2017

Khadem-Maaref, M.; Mehrnejad, F.; Phirouznia, A.

J. Mol. Graph. Model.

73

24-29

757448

29331879

Structural and quantum mechanical computations to elucidate the altered binding mechanism of metal and drug with pyrazinamidase from Mycobacterium tuberculosis due to mutagenicity

2018

Rasool, N.; Iftikhar, S.; Amir, A.; Hussain, W.

J. Mol. Graph. Model.

80

126-131

757448

29331879

Structural and quantum mechanical computations to elucidate the altered binding mechanism of metal and drug with pyrazinamidase from Mycobacterium tuberculosis due to mutagenicity

2018

Rasool, N.; Iftikhar, S.; Amir, A.; Hussain, W.

J. Mol. Graph. Model.

80

126-131

758283

25972249

Biochemical characterization and computational identification of Mycobacterium tuberculosis pyrazinamidase in some pyrazinamide-resistant isolates of Iran

2015

Doustdar, F.; Pazhang, M.; Mehrnejad, F.; Safarzadeh, M.; Rabiei, D.; Chaparzadeh, N.; Falahati, H.; Mir-Derikvand, M.

Protein J.

34

181-192

758283

25972249

Biochemical characterization and computational identification of Mycobacterium tuberculosis pyrazinamidase in some pyrazinamide-resistant isolates of Iran

2015

Doustdar, F.; Pazhang, M.; Mehrnejad, F.; Safarzadeh, M.; Rabiei, D.; Chaparzadeh, N.; Falahati, H.; Mir-Derikvand, M.

Protein J.

34

181-192

758499

28210279

Insights into pyrazinamidase and DNA gyrase protein structures in resistant and susceptible clinical isolates of Mycobacterium tuberculosis

2016

Ahmadi, A.; Nazari, R.; Arjomandzadegan, M.; Zolfaghari, M.R.; Vahidi, V.; Poolad, T.; Kahbazi, M.; Sadrnia, M.; Tousheh, M.; Rafiee, P.

Tanaffos

15

147-153

758499

28210279

Insights into pyrazinamidase and DNA gyrase protein structures in resistant and susceptible clinical isolates of Mycobacterium tuberculosis

2016

Ahmadi, A.; Nazari, R.; Arjomandzadegan, M.; Zolfaghari, M.R.; Vahidi, V.; Poolad, T.; Kahbazi, M.; Sadrnia, M.; Tousheh, M.; Rafiee, P.

Tanaffos

15

147-153

764624

31951850

Discovery of hydrazone containing thiadiazoles as Mycobacterium tuberculosis growth and enoyl acyl carrier protein reductase (InhA) inhibitors

2020

Dogan, H.; Dogan, S.D.; Guenduez, M.G.; Krishna, V.S.; Lherbet, C.; Sriram, D.; Sahin, O.; Saripinar, E.

Eur. J. Med. Chem.

188

112035

764624

31951850

Discovery of hydrazone containing thiadiazoles as Mycobacterium tuberculosis growth and enoyl acyl carrier protein reductase (InhA) inhibitors

2020

Dogan, H.; Dogan, S.D.; Guenduez, M.G.; Krishna, V.S.; Lherbet, C.; Sriram, D.; Sahin, O.; Saripinar, E.

Eur. J. Med. Chem.

188

112035

757448

2 entries

758283

2 entries

758499

2 entries

764624

2 entries

772685

31636108

Metallochaperones are needed for Mycobacterium tuberculosis and Escherichia coli nicotinamidase-pyrazinamidase activity

2020

Sheen, P.; Monsalve, A.; Campos, J.; Huerta, R.; Antiparra, R.; Arteaga, H.; Duran, P.; Bueno, C.; Kirwan, D.E.; Gilman, R.H.; Zimic, M.

J. Bacteriol.

202

e00331

773014

32340563

Structural insights of catalytic mechanism in mutant pyrazinamidase of Mycobacterium tuberculosis

2020

Junaid, M.; Li, C.-D.; Li, J.; Khan, A.; Shujait Ali, S.; Jamal, S.B.; Saud, S.; Ali, A.; Wei, D.

J. Biomol. Struct. Dyn.

39

3172-3185

Links to other databases for Pyrazinamide

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