Ligand tRNA

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Basic Ligand Information

Molecular Structure
Picture of tRNA (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C38H48N15O29P4R
tRNA
WFCSZNSYAGQHAH-GQHWQGICSA-N
Synonyms:
t-RNA, tRNA-guanine, tRNA1, tRNA guanine, [tRNA]-guanine
Pathway Source
Pathways


Show all pahtways known for Show all BRENDA pathways known for tRNA

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (3 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
L-threonylcarbamoyl-AMP + tRNA = t6A37-tRNA
show the reaction diagram
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In Vivo Product in Enzyme-catalyzed Reactions (14 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
L-phenylalanyl-tRNA + protein = tRNA + L-phenylalanyl-protein
show the reaction diagram
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tRNAn+1 + phosphate = tRNA + a nucleoside diphosphate
show the reaction diagram
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tRNA containing extra residues at the 3'-terminus = 5'-mononucleotide + tRNA
show the reaction diagram
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Substrate in Enzyme-catalyzed Reactions (17 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
L-threonylcarbamoyl-AMP + tRNA = t6A37-tRNA
show the reaction diagram
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tRNA + H2O = tRNA fragments + nucleoside 3'-phosphates
show the reaction diagram
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tRNA + H2O = 5'-phosphooligonucleotides
show the reaction diagram
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tRNA + H2O = 5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
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tRNA + H2O = ?
show the reaction diagram
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ATP + L-cysteine + tRNA = AMP + diphosphate + L-cysteinyl-tRNA
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ATP + L-glutamate + tRNA = AMP + diphosphate + L-glutamyl-tRNA
show the reaction diagram
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Product in Enzyme-catalyzed Reactions (107 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
tRNA-1-methylguanine + 2-oxoglutarate + O2 = tRNA-guanine + formaldehyde + succinate + CO2
show the reaction diagram
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L-seryl-tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine = tRNA + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-seryl)-L-lysyl-D-alanyl-D-alanine
show the reaction diagram
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N-acetylmuramoyl-L-alanyl-D-isoglutaminyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine + 2 glycyl-tRNA = N-acetylmuramoyl-L-alanyl-D-isoglutaminyl-L-lysyl-(N6-triglycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine + 2 tRNA
show the reaction diagram
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N-acetylmuramoyl-L-alanyl-D-isoglutaminyl-L-lysyl-(N6-triglycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine + 2 glycyl-tRNA = N-acetylmuramoyl-L-alanyl-D-isoglutaminyl-L-lysyl-(N6-pentaglycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine + 2 tRNA
show the reaction diagram
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L-arginyl-tRNA + L-Asp-beta-galactosidase = tRNA + L-arginyl-L-Asp-beta-galactosidase
show the reaction diagram
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tRNAn+1 + phosphate = tRNA + a nucleoside diphosphate
show the reaction diagram
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tRNA containing extra residues at the 3'-terminus = 5'-mononucleotide + tRNA
show the reaction diagram
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precursor tRNATyr + H2O = tRNA + 3' trailer
show the reaction diagram
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Activator in Enzyme-catalyzed Reactions (4 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
uncharged or charged, stimulation, level of stimulation is greater in presence of RNA and poly(U) together than with either RNA alone
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stimulates
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stimulates l-cysteine activation 2-3fold
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GluRS is one of the aminoacyl-tRNA synthetases that require the cognate tRNA for specific amino acid recognition and activation, tRNA serves as the enzyme activator in the first step, and as the substrate in the second step of aminoacylation, overview, On the other hand, the main chain of the glutamate is immature glutamate-binding site in the absence of tRNA
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Inhibitor in Enzyme-catalyzed Reactions (17 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
half-maximal inhibition rate at 0.08 mM
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inhibits enzyme activity and inhibition by ethidium bromide
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10 nM, strong competitive inhibition of 2',3'-cyclic phosphodiesterase activity, inhibition is abolished by addition of Mg2+, Mn2+ or Ca2+, but not of Ni2+
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wild-type RNA, 50% inhibition at a concentration of 0.005 mM
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activity is inhibited by single tRNAs or bulk tRNA from all sources tested, and by ribosomal RNA
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above 0.4 mg/ml
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above 0.6 mg/ml
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partly inhibits the binding of diphosphate
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Enzyme Kinetic Parameters

kcat Value (Turnover Number) (13 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE

KM Value (22 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.013
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from Lactobacillus
0.0027
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pH 7.5, 37°C, sulfur deficient tRNA
0.00025
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pH 7.5, 77°C
0.023
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mutant AlaRS(K73E), aminoacylation

Ki Value (9 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.000095
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pH 7.5, 50°C, unfractionated Escherichia coli tRNA; unfractionated tRNA from Escherichia coli, 50°C, pH 7.5
0.005
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wild-type RNA

References & Links

Links to other databases for tRNA

ChEBI
PubChem
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PubChem