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Ligand Tetrapolyphosphate

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (14 results)

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Show Substrate (14 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.7.1.23

NAD+ + poly(P)4 = NADP+ + poly(P)3

662376

-

2.7.1.23

NADH + poly(P)4 = NADPH + poly(P)3

691390

-

2.7.1.23

tetraphosphate + NAD+ = triphosphate + NADP+

721366

-

2.7.1.23

tetrapolyphosphate + NAD+ = tripolyphosphate + NADP+

723257

-

2.7.1.23

4 entries

2.7.1.63

(phosphate)4 + D-glucose = (phosphate)3 + D-glucose 6-phosphate

721367

-

2.7.1.86

tetrapolyphosphate + NADH = tripolyphosphate + NADPH

662376

-

2.7.7.9

UDP-glucose + tetrapolyphosphate = uridine-5'-pentaphosphate + D-glucose-1-phosphate

661732

-

2.7.99.1

tetraphosphate + [microsomal membrane protein] = triphosphate + phosphorylated [microsomal membrane protein]

662083

-

3.1.3.2

tetrapolyphosphate + H2O = ?

653570, 651143

-

3.6.1.1

tetrapolyphosphate + H2O = ?

209796, 209768, 728748

-

3.6.1.11

(phosphate)4 + H2O = (phosphate)3 + phosphate

696296

-

3.6.1.11

tetraphosphate + H2O = triphosphate + phosphate

701387

-

3.6.1.11

2 entries

6.2.1.1

ATP + tetrapolyphosphate = adenosine 5'-pentaphosphate

577

-

6.2.1.12

4-coumarate + MgATP2- + tetrapolyphosphate = adenosine 5'-pentaphosphate + ?

653536

-

Product in Enzyme-catalyzed Reactions (1 result)

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Show Product (1 entry)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.7.1.63

(phosphate)5 + D-glucose = (phosphate)4 + D-glucose 6-phosphate

-

-

Activator in Enzyme-catalyzed Reactions (2 results)

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Show Activating Compound (2 entries)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

2.4.2.31

10 mM, stimulates

638099

-

2.7.4.1

activation, removes lag-phase in synthesis at low ATP-levels, not phosphate, diphosphate or tripolyphosphate

645115

-

Inhibitor in Enzyme-catalyzed Reactions (8 results)

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Show Inhibitors (8 entries)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

2.5.1.6

-

639050

-

2.5.1.6

10 mM, 40% inhibition, S-adenosylmethionine synthetase B; 10 mM, 50% inhibition, S-adenosylmethionine synthetase A

639048

-

2.5.1.6

2 entries

2.7.1.63

inhibits reaction with ATP

641750

-

2.7.1.90

-

642081

-

2.7.4.33

-

719681

-

2.7.4.33

inhibits the hydrolysis of high-molecular polyphosphate

721769

-

2.7.4.33

mixed type inhibition with polyphosphate as substrate

719681

-

2.7.4.33

3 entries

3.6.1.11

50% inhibition at 2000fold polymer molar excess over the long-chain polyP substrate

209844

-

Metals and Ions (1 result)

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Show Metals Ions (1 entry)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

3.4.21.53

activation, only peptide hydrolysis, in the presence of Mg2+

29343

-

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (5 results)

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Show Turnover Number (5 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

2.7.1.23

8.7

-

in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C

721366

2.7.1.63

2.5

-

pH 7.0, temperature not specified in the publication

721367

3.6.1.11

0.9

-

in 50 mM PIPES, pH 6.8 containing 25 mM KCl and 2 mM MgCl2

701387

3.6.1.11

3.4

-

2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA

696296

3.6.1.11

7.1

-

2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA

696296

3.6.1.11

3 entries

K_M Value (13 results)

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Show KM Value (13 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.7.1.23

0.21

-

pH 7.0, 37°C, mutant G183R

662376

2.7.1.23

0.33

-

pH 7.0, 37°C, wild-type

662376

2.7.1.23

2.1

-

in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C

721366

2.7.1.23

12

-

in 100 mM Tris-HCl (pH 8.0), at 37°C

723257

2.7.1.23

4 entries

2.7.1.63

0.5

-

pH 7.0, temperature not specified in the publication

721367

2.7.1.86

0.58

-

wild type enzyme

662376

2.7.1.86

0.68

-

mutant enzyme G183R

662376

3.1.3.2

1.14

-

pH 5.8, 25°C

653570

3.1.3.2

1.9

-

pH 5.3, 25°C, SAP1

651143

3.1.3.2

2.9

-

pH 5.3, 25°C, SAP2

651143

3.6.1.11

0.019

-

2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA

696296

3.6.1.11

0.041

-

2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA

696296

3.6.1.11

0.11

-

in 50 mM PIPES, pH 6.8 containing 25 mM KCl and 2 mM MgCl2

701387

2.7.1.86

2 entries

3.1.3.2

3 entries

3.6.1.11

3 entries

K_i Value (1 result)

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Show KI Value (1 entry)

EC NUMBER

KI VALUE [MM]

KI VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.5.1.6

0.18

-

-

639050

References & Links

Literature References (26)

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Show Reference (26 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

577

7910605

Adenosine 5'-tetraphosphate and adenosine 5'-pentaphosphate are synthesized by yeast acetyl coenzyme A synthetase

1994

Guranowski, A.; Gunther Sillero, M.A.; Sillero, A.

J. Bacteriol.

176

2986-2990

29343

2931432

The role of ATP hydrolysis in the breakdown of proteins and peptides by protease La from Escherichia coli

1985

Goldberg, A.L.; Waxman, L.

J. Biol. Chem.

260

12029-12034

209768

2829731

Properties and regulation of Mg2+-dependent chloroplast inorganic pyrophosphatase from Sorghum vulgare leaves

1988

Krishnan, V.A.; Gnanam, A.

Arch. Biochem. Biophys.

260

277-284

209796

-

Inorganic Pyrophosphatase of Escherichia coli

1971

Josse, J.; Wong, S.C.K.

The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )

4

499-527

209844

8380170

An exopolyphosphatase of Escherichia coli. The enzyme and its ppx gene in a polyphosphate operon

1993

Akiyama, M.; Crooke, E.; Kornberg, A.

J. Biol. Chem.

268

633-639

638099

6285835

Effects of nucleotides on activity of a purified ADP-ribosyltransferase from turkey erythrocytes

1982

Watkins, P.A.; Moss, J.

Arch. Biochem. Biophys.

216

74-80

639048

3142771

S-adenosylmethionine synthetase in the thermophilic archaebacterium Sulfolobus solfataricus. Purification and characterization of two isoforms

1988

Porcelli, M.; Cacciapuoti, G.; Carteni-Farina, M.; Gambacorta, A.

Eur. J. Biochem.

177

273-280

639050

4586937

Inhibition of ATP: L-methionine S-adenosyltransferase of bakers yeast by structural analogues of ATP

1973

Chou, T.C.; Talalay, P.

Biochim. Biophys. Acta

321

467-474

641750

8639629

Initial rate and equilibrium isotope exchange studies on the ATP-dependent activity of polyphosphate Glucokinase from Propionibacterium shermanii

1996

Kowalczyk, T.H.; Horn, P.J.; Pan, W.H.; Phillips, N.F.

Biochemistry

35

6777-6785

642081

6091737

Kinetic mechanism of pyrophosphate-dependent phosphofructokinase from Propionibacterium freudenreichii

1984

Bertagnolli, B.L.; Cook, P.F.

Biochemistry

23

4101-4108

645115

2164013

Polyphosphate kinase from Escherichia coli. Purification and demonstration of a phosphoenzyme intermediate

1990

Ahn, K.; Kornberg, A.

J. Biol. Chem.

265

11734-11739

651143

12473124

Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures

2002

Bozzo, G.G.; Raghothama, K.G.; Plaxton, W.C.

Eur. J. Biochem.

269

6278-6286

653536

12644689

4-Coumarate:coenzyme A ligase has the catalytic capacity to synthesize and reuse various (di)adenosine polyphosphates

2003

Pietrowska-Borek, M.; Stuible, H.P.; Kombrink, E.; Guranowski, A.

Plant Physiol.

131

1401-1410

653570

11800388

Purification and characterization of banana fruit acid phosphatase

2001

Turner, W.L.; Plaxton, W.C.

Planta

214

243-249

661732

15013755

Uridine 5'-polyphosphates (p4U and p5U) and uridine(5')polyphospho(5')nucleosides (Up(n)Ns) can be synthesized by UTP:glucose-1-phosphate uridylyltransferase from Saccharomyces cerevisiae

2004

Guranowski, A.; de Diego, A.; Sillero, A.; Gunther Sillero, M.A.

FEBS Lett.

561

83-88

662083

3949741

Tripolyphosphate is an alternative phosphodonor of the selective protein phosphorylation of liver microsomal membrane

1986

Tsutsui, K.

J. Biol. Chem.

261

2645-2653

662376

15855156

Molecular conversion of NAD Kinase to NADH kinase through single amino acid residue substitution

2005

Mori, S.; Kawai, S.; Shi, F.; Mikami, B.; Murata, K.

J. Biol. Chem.

280

24104-24112

691390

18391451

Structure and function of NAD kinase and NADP phosphatase: key enzymes that regulate the intracellular balance of NAD(H) and NADP(H)

2008

Kawai, S.; Murata, K.

Biosci. Biotechnol. Biochem.

72

919-930

696296

18700747

Human metastasis regulator protein H-prune is a short-chain exopolyphosphatase

2008

Tammenkoski, M.; Koivula, K.; Cusanelli, E.; Zollo, M.; Steegborn, C.; Baykov, A.A.; Lahti, R.

Biochemistry

47

9707-9713

701387

19304823

Exopolyphosphatases PPX1 and PPX2 from Corynebacterium glutamicum[down-pointing small open triangle]

2009

Lindner, S.N.; Knebel, S:; Wesseling,H.; Schoberth, S.M.; Wendisch, V.F.

Appl. Environ. Microbiol.

75

3161-3170

719681

1655714

Properties of polyphosphate:AMP phosphotransferase of Acinetobacter strain 210A

1991

Bonting, C.F.C.; Kortstee, G.J.J.; Zehnder, A.J.B.

J. Bacteriol.

173

6484-6488

721366

20180116

Polyphosphate/ATP-dependent NAD kinase of Corynebacterium glutamicum: biochemical properties and impact of ppnK overexpression on lysine production

2010

Lindner, S.N.; Niederholtmeyer, H.; Schmitz, K.; Schoberth, S.M.; Wendisch, V.F.

Appl. Microbiol. Biotechnol.

87

583-593

721367

20379711

Cg2091 encodes a polyphosphate/ATP-dependent glucokinase of Corynebacterium glutamicum

2010

Lindner, S.N.; Knebel, S.; Pallerla, S.R.; Schoberth, S.M.; Wendisch, V.F.

Appl. Microbiol. Biotechnol.

87

703-713

721769

11068824

Purification and properties of pyrophosphatase of Acinetobacter johnsonii 210A and its involvement in the degradation of polyphosphate

1999

Bonting, C.; Gerards, R.; Zehnder, A.; Kortstee, G.

Biodegradation

10

393-398

723257

23212377

Identification and characterization of a human mitochondrial NAD kinase

2012

Ohashi, K.; Kawai, S.; Murata, K.

Nat. Commun.

3

1248

728748

24755063

Cloning, purification, and characterization of inorganic pyrophosphatase from the hyperthermophilic archaea Pyrococcus horikoshii

2014

Lu, D.; Xie, G.; Gao, R.

Protein Expr. Purif.

99

94-98

Links to other databases for Tetrapolyphosphate

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ChEBI

PubChem

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