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Ligand 2-Nitropropane

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (9 results)

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Show Substrate (9 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.13.12.16

2-nitropropane + O2 + H2O = acetone + HNO2 + H2O2

639225

-

1.13.12.16

2-nitropropane + O2 = ?

695891

-

1.13.12.16

2-nitropropane + O2 = ? + HNO2

639233

-

1.13.12.16

2-nitropropane + O2 = acetone + HNO2

639232, 728077, 639225, 639222, 639223, 639226, 639235, 656289, 674590, 724175, 639224, 639227

-

1.13.12.16

2-nitropropane + O2 = acetone + nitrite

656289

-

1.7.3.1

2-nitropropane + H2O + O2 = acetone + nitrite + H2O2

395013

-

1.7.3.1

2-nitropropane + H2O = propanone + nitrite + H2O2

724175

-

1.7.3.1

2-nitropropane + O2 = acetone + HNO2

639230

-

1.7.3.1

2-nitropropane + O2 = acetone + nitrite

654373

-

1.13.12.16

5 entries

1.7.3.1

4 entries

3D Structure of Enzyme-Ligand-Complex (PDB) (1 result)

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Show 3D Structure of Enzyme-Ligand-Complex (PDB) (1 entry)

EC NUMBER

ENZYME 3D STRUCTURE

1.13.11.32

2gjn, 3D-view

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (3 results)

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Show Turnover Number (3 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

1.13.12.16

0.82

-

pH 8.0, 30°C

656289

1.13.12.16

160

-

neutral form of substrate

639232

1.13.12.16

6400

-

anionic form of substrate

639232

1.13.12.16

3 entries

K_M Value (11 results)

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Show KM Value (11 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.13.12.16

1.61

-

anionic form of substrate

639222, 639227

1.13.12.16

1.61

-

anionic form of the substrate

639222

1.13.12.16

3.1

-

anionic form of substrate

639232

1.13.12.16

6

-

anionic form of substrate

639232

1.13.12.16

7.4

-

-

639225

1.13.12.16

19.2

-

-

639224

1.13.12.16

21.3

-

-

639223

1.13.12.16

21.3

-

neutral form of substrate

639222

1.13.12.16

21.3

-

neutral form of the substrate

639222

1.13.12.16

33

-

neutral form of substrate

639232

1.13.12.16

10 entries

1.7.3.1

83.5

-

pH 8.0, 28°C

724175

References & Links

Literature References (17)

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Show Reference (17 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

395013

14907722

Oxidation of nitroethane by extracts from Neurospora

1951

Little, H.N.

J. Biol. Chem.

193

347-358

639222

-

2-Nitropropane dioxygenase from Hansenula mrakii: re-characterization of the enzyme and oxidation of anionic nitroalkanes

1984

Kido, T.; Tanizawa, K.; Inagaki, K.; Yoshimura, T.; Ishida, M.; Hashizume, K.; Soda, K.

Agric. Biol. Chem.

48

2549-2554

639223

11214

A new oxygenase, 2-nitropropane dioxygenase of Hansenula mrakii. Enzymologic and spectrophotometric properties

1976

Kido, T.; Soda, K.; Suzuki, T.; Asada, K.

J. Biol. Chem.

251

6994-7000

639224

201619

Properties of 2-nitropropane dioxygenase of Hansenula mrakii. Formation and participation of superoxide

1978

Kido, T.; Soda, K.; Asada, K.

J. Biol. Chem.

253

226-232

639225

22538

Purification and properties of nitroalkane oxidase from Fusarium oxysporum

1978

Kido, T.; Hashizume, K.; Soda, K.

J. Bacteriol.

133

53-58

639226

-

Characterization of primary nitroalkane oxidation by 2-nitropropane dioxygenase

1984

Kido, T.; Tanizawa, K.; Ishida, M.; Inagaki, K.; Soda, K.

Agric. Biol. Chem.

48

1361-1362

639227

6149727

Oxidation of anionic nitroalkanes by flavoenzymes, and participation of superoxide anion in the catalysis

1984

Kido, T.; Soda, K.

Arch. Biochem. Biophys.

234

468-475

639230

10684620

Iso-mechanism of nitroalkane oxidase: 1. Inhibition studies and activation by imidazole

2000

Gadda, G.; Fitzpatrick, P.F.

Biochemistry

39

1400-1405

639232

9501443

Purification, characterization, and mechanism of a flavin mononucleotide-dependent 2-nitropropane dioxygenase from Neurospora crassa

1998

Gorlatova, N.; Tchorzewski, M.; Kurihara, T.; Soda, K.; Esaki, N.

Appl. Environ. Microbiol.

64

1029-1033

639233

10068453

Substrate specificity of a nitroalkane-oxidizing enzyme

1999

Gadda, G.; Fitzpatrick, P.F.

Arch. Biochem. Biophys.

363

309-313

639235

947888

Purification and properties of nitroalkane-oxidizing enzyme from Hansenula mrakii

1976

Kido, T.; Yamamoto, T.; Soda, K.

J. Bacteriol.

126

1261-1265

654373

15581574

Nitroalkane oxidase, a carbanion-forming flavoprotein homologous to acyl-CoA dehydrogenase

2005

Fitzpatrick, P.F.; Orville, A.M.; Nagpal, A.; Valley, M.P.

Arch. Biochem. Biophys.

433

157-165

656289

15582992

Involvement of a flavosemiquinone in the enzymatic oxidation of nitroalkanes catalyzes by 2-nitropropane dioxygenase

2005

Francis, K.; Russell, B.; Gadda, G.

J. Biol. Chem.

280

5195-5204

674590

16682407

Crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate. Identification of the catalytic base

2006

Ha, J.Y.; Min, J.Y.; Lee, S.K.; Kim, H.S.; Kim, d.o.J.; Kim, K.H.; Lee, H.H.; Kim, H.K.; Yoon, H.J.; Suh, S.W.

J. Biol. Chem.

281

18660-18667

695891

19577534

Nitronate monooxygenase, a model for anionic flavin semiquinone intermediates in oxidative catalysis

2009

Gadda, G.; Francis, K.

Arch. Biochem. Biophys.

493

53-61

724175

21147069

Crystal structure and site-directed mutagenesis of a nitroalkane oxidase from Streptomyces ansochromogenes

2011

Li, Y.; Gao, Z.; Hou, H.; Li, L.; Zhang, J.; Yang, H.; Dong, Y.; Tan, H.

Biochem. Biophys. Res. Commun.

405

344-348

728077

23408846

Rv1894c is a novel hypoxia-induced nitronate monooxygenase required for Mycobacterium tuberculosis virulence

2013

Klinkenberg, L.G.; Karakousis, P.C.

J. Infect. Dis.

207

1525-1534

Links to other databases for 2-Nitropropane

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ChEBI

PubChem

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