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Ligand 2-amino-5-formylamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Basic Ligand Information Molecular Structure
C1 0 H1 6 N5 O9 P
2-amino-5-formylamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine
VKMYTDDVUBGBDH-UUOKFMHZSA-N
2-amino-5-formylamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one, 2-amino-5-formylamino-6-(5-phosphoribosylamino)pyrimidin-4(3H)-one, 2-amino-5-formylamino-6-(D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate
Show all BRENDA pathways known for 2-amino-5-formylamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine
Roles as Enzyme Ligand
In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)
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2-amino-5-formylamino-6-(D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + H2O = 2,5-diamino-6-(D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + formate
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Substrate in Enzyme-catalyzed Reactions (1 result)
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2-amino-5-formylamino-6-(D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + H2O = 2,5-diamino-6-(D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + formate
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Product in Enzyme-catalyzed Reactions (4 results)
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GTP + 3 H2O = 2-amino-5-formylamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + 2 phosphate
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beta,gamma-methylene-GTP + H2O = 2-amino-5-formylamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + methyl phosphonate
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GTP + H2O = 2-amino-5-formylamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + phosphate
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GTP-gamma-S + H2O = 2-amino-5-formylamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + ?
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Enzyme Kinetic Parameters
KM Value (1 result)
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1
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pH 7.2, 70°C, apparent Km-value is about 1 mM at a concentration of 2 mM Fe2+, type of curve is typically indicative of homomeric cooperativity and suggests that ArfB may exhibit positive cooperative substrate binding
References & Links Literature References (2)
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A member of a new class of GTP cyclohydrolases produces formylaminopyrimidine nucleotide monophosphates
2002
Graham, D.E.; Xu, H.; White, R.H.
Biochemistry
41
15074-15084
An iron(II) dependent formamide hydrolase catalyzes the second step in the archaeal biosynthetic pathway to riboflavin and 7,8-didemethyl-8-hydroxy-5-deazariboflavin
2009
Grochowski, L.L.; Xu, H.; White, R.H.
Biochemistry
48
4181-4188
Links to other databases for 2-amino-5-formylamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine