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Basic Ligand Information
Roles as Enzyme Ligand
Enzyme Kinetic Parameters
References & Links
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Ligand 5alpha-cholesta-8,14-dien-3beta-ol

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Basic Ligand Information

Molecular Structure

Picture of 5alpha-cholesta-8,14-dien-3beta-ol (click for magnification)

Molecular Formula

BRENDA Name

InChIKey

C₂₇H₄₂O

5alpha-cholesta-8,14-dien-3beta-ol

PYVGNKOAUJOCLS-CXDHQSPESA-N

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)

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Show Natural Substrate (1 entry)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.3.1.70

5alpha-cholesta-8,14-dien-3beta-ol + NADPH = 5alpha-cholesta-8-en-3beta-ol + NADP+

654166

Substrate in Enzyme-catalyzed Reactions (2 results)

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EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.3.1.70

5alpha-cholesta-8,14-dien-3beta-ol + NADPH + H+ = 5alpha-cholesta-8-en-3beta-ol + NADP+

745882, 672378

1.3.1.70

5alpha-cholesta-8,14-dien-3beta-ol + NADPH = 5alpha-cholesta-8-en-3beta-ol + NADP+

654166, 288490, 288492, 288493

1.3.1.70

2 entries

Enzyme Kinetic Parameters

K_M Value (1 result)

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Show KM Value (1 entry)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.3.1.70

0.45

288493

References & Links

Literature References (6)

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Show Reference (6 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

288490

6444198

Microsomal enzymes of cholesterol biosynthesis from lanosterol. Characterization, solubilization, and partial purification of NADPH-dependent DELTA8,14-steroid 14-reductase

1984

Paik, Y.K.; Trzaskos, J.M.; Shafiee, A.; Gaylor, J.L.

J. Biol. Chem.

259

13413-13423

288492

11784322

Cloning and expression of sterol DELTA14-reductase from bovine liver

2002

Roberti, R.; Bennati, A.M.; Galli, G.; Caruso, D.; Maras, B.; Aisa, C.; Beccari, T.; Della Fazia, M.A.; Servillo, G.

Eur. J. Biochem.

269

283-290

288493

2591378

Microsomal DELTA8,14-sterol DELTA14-reductase in higher plants. Characterization and inhibition by analogues of a presumptive carbocationic intermediate of the reduction reaction

1989

Taton, M.; Benveniste, P.; Rahier, A.

Eur. J. Biochem.

185

605-614

654166

12618959

Autosomal recessive HEM/Greenberg skeletal dysplasia is caused by 3beta-hydroxysterol DELTA14-reductase deficiency due to mutations in the lamin B receptor gene

2003

Waterham, H.R.; Koster, J.; Mooyer, P.; Noort Gv, G.; Kelley, R.I.; Wilcox, W.R.; Wanders, R.J.; Hennekam, R.C.; Oosterwijk, J.C.

Am. J. Hum. Genet.

1013-1017

672378

16784888

Sterol dependent regulation of human TM7SF2 gene expression: role of the encoded 3beta-hydroxysterol DELTA14-reductase in human cholesterol biosynthesis

2006

Bennati, A.M.; Castelli, M.; Della Fazia, M.A.; Beccari, T.; Caruso, D.; Servillo, G.; Roberti, R.

Biochim. Biophys. Acta

1761

677-685

745882

25307054

Structure of an integral membrane sterol reductase from Methylomicrobium alcaliphilum

2015

Li, X.; Roberti, R.; Blobel, G.

Nature

517