Ligand ribothymidine

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Basic Ligand Information

Molecular Structure
Picture of ribothymidine (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C10H14N2O6
ribothymidine
DWRXFEITVBNRMK-JXOAFFINSA-N
Synonyms:
1-beta-D-ribofuranosylthymine, 2-hydroxy-thymidine, 5-C-methyluridine, 5-methyluridine, methyluridine, ribo-thymidine, ribofuranosyl thymine, Ribosylthymidine, ribosylthymine, thymine ribonucleoside, thymine riboside

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (2 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
5-methyluridine + phosphate = 5-methyluracil + alpha-D-ribose 1-phosphate
show the reaction diagram
-
5-methyluridine + phosphate = 5-methyluracil + alpha-D-ribose 1-phosphate
show the reaction diagram
-

In Vivo Product in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
thymidine + 2-oxoglutarate + O2 = 5-methyluridine + succinate + CO2
show the reaction diagram
-

Substrate in Enzyme-catalyzed Reactions (15 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
5-methyluridine + H2O = 5-methyluracil + D-ribose
show the reaction diagram
-
ribosylthymine + H2O = ?
show the reaction diagram
-
5-methyluridine + H2O = D-ribose + 5-methyluracil
show the reaction diagram
-
5-methyluridine + H2O = 5-methyluracil + D-ribose
show the reaction diagram
-

Product in Enzyme-catalyzed Reactions (4 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
thymidine + 2-oxoglutarate + O2 = 5-methyluridine + succinate + CO2
show the reaction diagram
-
inosine + thymine = hypoxanthine + 5-methyluridine
show the reaction diagram
-
-
ribo-TMP + H2O = ribo-thymidine + phosphate
show the reaction diagram
-
-
5-methylcytidine + H2O = 5-methyluridine + NH3
show the reaction diagram
-
-

Inhibitor in Enzyme-catalyzed Reactions (9 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
1 mM, about 95% inhibition
-

3D Structure of Enzyme-Ligand-Complex (PDB) (1 result)

EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (3 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
56
-
pH 7.0, 90°C
1.29
-
pH 7.2, 32°C, recombinant His-tagged enzyme
25.5
-
pH 7.3, 37°C

KM Value (16 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.2
-
-
0.44
-
pH and temperature not specified in the publication

Ki Value (3 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
2
-
pH 7.5, 37°C, wild-type enzyme, versus uridine

References & Links

Links to other databases for ribothymidine

ChEBI
PubChem
ChEBI
PubChem