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Ligand hydrogen sulfide

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Basic Ligand Information

Molecular Structure
Picture of hydrogen sulfide (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
Molfile
H2S
hydrogen sulfide
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Synonyms:
bisulfide, H2S, HS-, hydrogen-sulfide, hydrogensulfide, hydrosulfide, SH-, SH2, Sulfide, sulphur hydride

Related pathways

Pathway Source
Pathways
BRENDA
cysteine metabolism, hydrogen production, sulfate reduction
KEGG
Carbon metabolism, Cysteine and methionine metabolism, Microbial metabolism in diverse environments, Sulfur metabolism
MetaCyc
4,4'-disulfanediyldibutanoate degradation, assimilatory sulfate reduction I, assimilatory sulfate reduction II, assimilatory sulfate reduction III, assimilatory sulfate reduction IV more


Show all pahtways known for Show all BRENDA pathways known for hydrogen sulfide

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (42 results)

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EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
hydrogen sulfide + ROOH = ?
show the reaction diagram
-
hydrogen sulfide + a [DsrC protein]-disulfide + acceptor + H2O = sulfite + a [DsrC protein]-dithiol + reduced acceptor + H+
show the reaction diagram
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hydrogen sulfide + 2 ferricytochrome c = sulfur + 2 ferrocytochrome c + 2 H+
show the reaction diagram
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hydrogen sulfide + a [DsrC protein]-dithiol + 2 quinone = a [DsrC protein]-trisulfide + 2 quinol
show the reaction diagram
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S-adenosyl-L-methionine + H2S = S-adenosyl-L-homocysteine + methanethiol
show the reaction diagram
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acetyl-CoA + hydrogen sulfide = CoA + thioacetate
show the reaction diagram
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NAD+ + glycine + sulfide = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H2O
show the reaction diagram
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O-acetyl-L-serine + sulfide = L-cysteine + acetate
show the reaction diagram
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O-phospho-L-seryl-tRNACys + sulfide = L-cysteinyl-tRNACys + phosphate
show the reaction diagram
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hydrogen sulfide + L-aspartate 4-semialdehyde + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ = L-homocysteine + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster
show the reaction diagram
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sulfide + tRNA-uridine = tRNA-4-thiouridine
show the reaction diagram
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In Vivo Product in Enzyme-catalyzed Reactions (53 results)

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EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
sulfur = H2S
show the reaction diagram
-
5 sulfur + 2 O2 + 2 H2O + 4 H+ = sulfite + thiosulfate + 2 sulfide
show the reaction diagram
-
-
cypemycin(1-18)-L-Cys-L-Leu-L-Val-L-Cys + acceptor = C3.19,S21-cyclocypemycin(1-18)-L-Ala-L-Leu-N-thioethenyl-L-valinamide + CO2 + H2S + reduced acceptor
show the reaction diagram
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sulfite + 6 ferrocytochrome c + 6 H+ = H2S + 3 H2O + 6 ferricytochrome c
show the reaction diagram
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sulfur + NAD(P)H + H+ = hydrogen sulfide + NAD(P)+
show the reaction diagram
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(sulfide)n+1 + NADPH + H+ = hydrogen sulfide + (sulfide)n + NADP+
show the reaction diagram
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sulfite + 6 ferrocytochrome c + 6 H+ = sulfide + 6 ferricytochrome c + 3 H2O
show the reaction diagram
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thiosulfate + 2 ferrocytochrome c3 = sulfite + hydrogen sulfide + 2 ferricytochrome c3
show the reaction diagram
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a [DsrC protein]-trisulfide + 2 quinol = hydrogen sulfide + a [DsrC protein]-dithiol + 2 quinone
show the reaction diagram
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sulfite + reduced coenzyme F420 = hydrogen sulfide + oxidized coenzyme F420
show the reaction diagram
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3-mercaptopyruvate + cyanide = H2S + ?
show the reaction diagram
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thiosulfate + glutathione = sulfite + glutathione disulfide + sulfide
show the reaction diagram
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thiosulfate + 2 dithioerythritol = sulfite + dithioerythritol disulfide + sulfide
show the reaction diagram
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carbonyl sulfide + H2O = hydrogen sulfide + CO2
show the reaction diagram
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D-cysteine + H2O = sulfide + NH3 + pyruvate
show the reaction diagram
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L-cysteine + sulfite = L-cysteate + hydrogen sulfide
show the reaction diagram
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L-cysteine + H2O = sulfide + NH3 + pyruvate
show the reaction diagram
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D-cysteine + H2O = sulfide + NH3 + pyruvate
show the reaction diagram
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Substrate in Enzyme-catalyzed Reactions (198 results)

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EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
hydrogen sulfide + ROOH = ?
show the reaction diagram
-
hydrogen sulfide + a [DsrC protein]-disulfide + acceptor + H2O = sulfite + a [DsrC protein]-dithiol + reduced acceptor + H+
show the reaction diagram
-
sulfide + O2 = sulfur + H2O2
show the reaction diagram
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hydrogen sulfide + a [DsrC protein]-dithiol + 2 quinone = a [DsrC protein]-trisulfide + 2 quinol
show the reaction diagram
-
sulfite + hydrogen sulfide + menaquinone = thiosulfate + menaquinol
show the reaction diagram
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peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 H2S = peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + HS-SH
show the reaction diagram
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S-adenosyl-L-methionine + H2S = S-adenosyl-L-homocysteine + methanethiol
show the reaction diagram
-
acetyl-CoA + hydrogen sulfide = CoA + thioacetate
show the reaction diagram
-
NAD+ + glycine + sulfide = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H2O
show the reaction diagram
-
O-succinyl-L-homoserine + H2S = L-homocysteine + succinate
show the reaction diagram
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hydrogen sulfide + L-aspartate 4-semialdehyde + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ = L-homocysteine + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster
show the reaction diagram
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sulfide + adenylated-tRNA-uridine(position8) = tRNA-4-thiouridine(position8) + ?
show the reaction diagram
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3-phospho-L-serine + sulfide = ?
show the reaction diagram
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L-Seryl-tRNASec + sulfide = ?
show the reaction diagram
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horse heart cytochrome c + sulfide = ?
show the reaction diagram
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O-Acetyl-L-serine + H2S = Cysteine + acetate
show the reaction diagram
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L-homoserine + H2S = 2-oxobutyrate + NH3
show the reaction diagram
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O-acetyl-D-serine + sulfide = D-cysteine + ?
show the reaction diagram
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3-chloro-D-alanine + H2S = D-cysteine + HCl
show the reaction diagram
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Product in Enzyme-catalyzed Reactions (240 results)

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EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
H2 + sulfur = H2S
show the reaction diagram
-
-
5 sulfur + 2 O2 + 2 H2O + 4 H+ = sulfite + thiosulfate + 2 sulfide
show the reaction diagram
-
-
cypemycin(1-18)-L-Cys-L-Leu-L-Val-L-Cys + acceptor = C3.19,S21-cyclocypemycin(1-18)-L-Ala-L-Leu-N-thioethenyl-L-valinamide + CO2 + H2S + reduced acceptor
show the reaction diagram
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(sulfide)n+1 + NADPH + H+ = hydrogen sulfide + (sulfide)n + NADP+
show the reaction diagram
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sulfur + reduced benzyl viologen + H+ = sulfide + oxidized benzyl viologen
show the reaction diagram
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thiosulfate + menaquinol = sulfite + hydrogen sulfide + menaquinone
show the reaction diagram
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sulfur + reduced methyl viologen + H2O = H2S + oxidized methyl viologen
show the reaction diagram
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L-homocysteine + KCN = gamma-cyano-alpha-aminobutyric acid + H2S
show the reaction diagram
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L-cysteine + dithiothreitol = S-(2,3-hydroxy-4-thiobutyl)-L-cysteine + sulfide
show the reaction diagram
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thiosulfate + glutathione = sulfite + glutathione disulfide + sulfide
show the reaction diagram
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L-cysteine = L-alanine + sulfide
show the reaction diagram
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[protein]-N6-(octanoyl)-L-lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = [protein]-N6-[(R)-dihydrolipoyl]-L-lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin
show the reaction diagram
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carbonyl sulfide + H2O = hydrogen sulfide + CO2
show the reaction diagram
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benzoyl-thiocarbamic acid + H2O = N-benzoyl-Gly + SH2
show the reaction diagram
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D-cysteine + H2O = sulfide + NH3 + pyruvate
show the reaction diagram
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D-cysteine + H2O = sulfide + NH3 + pyruvate
show the reaction diagram
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Activator in Enzyme-catalyzed Reactions (17 results)

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EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
activates at 0.01 M
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activating at 0.1 mM, inhibitory above 0.3 mM, in human colon cancer cell line HT-29
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Inhibitor in Enzyme-catalyzed Reactions (76 results)

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EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
at high sulfide concentrations, enzymic inhibition is observed above 0.25 mM (20% inhibition at 0.3 mM sulfide)
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sulfide, elemental sulfur, elemental selenium or hydroxylamine required in catalytic amount, inhibition when added over a critical concentration (with the exception of hydroxylamine)
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reduction of sulfite, nitrite, hydroxylamine by reduced methyl viologen
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the enzyme activity decreases when the ambient sulfide concentration exceeds 0.3 mM
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Na2S at 0.5 mM inactivates completely
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product inhibition, competitive versus both substrates
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optimal sulfide concentration for 4-thiouridine modification of tRNAPhe is about 50 mM. Higher sulfide concentrations are inhibitory
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Zn2+ but not Cd2+, Ca2+ or Mg2+ could counteract the inhibitory effect
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1-3 mM, complete inhibition
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Metals and Ions (47 results)

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EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
activation, in vitro
-

3D Structure of Enzyme-Ligand-Complex (PDB) (866 results)

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EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (73 results)

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EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.0381
-
pH 7.0, 30°C
96.65
-
pH 7.5, 25°C

KM Value (172 results)

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EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.06
-
-
0.6
-
pH and temperature not specified in the publication
3
-
pH 7.3, 30°C, gamma-replacement reaction
8.8
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20°C, pH 9.2
1
-
pH 7.3, 37°C
46
-
-

Ki Value (27 results)

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EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.09
-
-
1.35
-
pH 7.5, 22°C
0.025
-
pH 8
0.076
-
-

IC50 Value (3 results)

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EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE
1
-
pH 7.5, 20°C, inhibition of CO2 hydration activity

References & Links

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Links to other databases for hydrogen sulfide

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EXTERNAL LINKS
ChEBI