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Ligand 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)

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Show Natural Substrate (1 entry)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.7.1.9

2-hydroxy-6-oxo-7-methylocta-2,4-dienoate + H2O = ?

751149

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Substrate in Enzyme-catalyzed Reactions (2 results)

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Show Substrate (2 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.7.1.9

2-hydroxy-6-oxo-7-methylocta-2,4-dienoate + H2O = ?

672388, 674958, 749943, 751149

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3.7.1.9

2-hydroxy-7-methyl-6-oxoocta-2,4-dienoate + H2O = ?

247051

-

3.7.1.9

2 entries

Product in Enzyme-catalyzed Reactions (3 results)

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Show Product (3 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.13.11.39

3-isoporopylcatechol + O2 = 2-hydroxy-7-methyl-6-oxo-octa-2,4-dienoate + ?

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-

1.13.11.39

3-isopropylcatechol + O2 = 2-hydroxy-7-methyl-6-oxo-octa-2,4-dienoate + ?

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-

1.13.11.39

3-ethylcatechol + O2 = 2-hydroxy-7-methyl-6-oxoocta-2,4-dienoate

-

-

1.13.11.39

3 entries

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (13 results)

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Show Turnover Number (13 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

3.7.1.9

0.41

-

-

672388

3.7.1.9

0.73

-

mutant A129/V227I

672388

3.7.1.9

3.3

-

mutant A129/I199V

672388

3.7.1.9

4.55

-

mutant A129/I199V

672388

3.7.1.9

6.4

-

mutant F104M

672388

3.7.1.9

6.7

-

mutant S34A

672388

3.7.1.9

8.1

-

mutant S34G

672388

3.7.1.9

20

-

mutant V227I

672388

3.7.1.9

21

-

150 mM phosphate buffer, at pH 7.5 and 25°C

751149

3.7.1.9

21

-

150 mM phosphate, 25°C, pH 7.5

674958

3.7.1.9

23.1

-

wild-type

672388

3.7.1.9

25

-

mutant A129V

672388

3.7.1.9

29

-

mutant I199V

672388

3.7.1.9

13 entries

K_M Value (12 results)

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Show KM Value (12 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

3.7.1.9

0.0008

-

mutant S34G

672388

3.7.1.9

0.0014

-

mutant I199V

672388

3.7.1.9

0.0035

-

mutant A129/I199V

672388

3.7.1.9

0.004

-

mutant F104M

672388

3.7.1.9

0.0049

-

mutant V227I

672388

3.7.1.9

0.005

-

mutant S34A

672388

3.7.1.9

0.0056

-

mutant A129V

672388

3.7.1.9

0.0073

-

150 mM phosphate buffer, at pH 7.5 and 25°C

751149

3.7.1.9

0.0073

-

150 mM phosphate, 25°C, pH 7.5

674958

3.7.1.9

0.0078

-

wild-type

672388

3.7.1.9

0.01

-

mutant A129/V227I

672388

3.7.1.9

0.022

-

-

672388

3.7.1.9

12 entries

References & Links

Literature References (5)

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Show Reference (5 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

247051

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The purification and properties of two isofunctional 2-hydroxy-6-oxohepta-2,4-dienoate hydrolase from Alcaligenes eutrophus strain 345

1987

Bayly, R.C.; Diberardino, D.; Hughes, E.J.L.; Skurray, R.A.

J. Gen. Microbiol.

133

815-822

672388

16844437

Improving the catalytic efficiency of a meta-cleavage product hydrolase (CumD) from Pseudomonas fluorescens IP01

2006

Jun, S.; Fushinobu, S.; Nojiri, H.; Omori, T.; Shoun, H.; Wakagi, T.

Biochim. Biophys. Acta

1764

1159-1166

674958

16233251

Purification, characterization, and steady-state kinetics of a meta-cleavage compound hydrolase from Pseudomonas fluorescens IPO1

2002

Saku, T.; Fushinobu, S.; Jun, J.S.; Ikeda, N.; Nojiri, H.; Yamane, H.; Omori, T.; Wakagi, T.

J. Biosci. Bioeng.

93

568-574

749943

16844437

Improving the catalytic efficiency of a meta-cleavage product hydrolase (CumD) from Pseudomonas fluorescens IP01

2006

Jun, S.Y.; Fushinobu, S.; Nojiri, H.; Omori, T.; Shoun, H.; Wakagi, T.

Biochim. Biophys. Acta

1764

1159-1166

751149

16233251

Purification, characterization, and steady-state kinetics of a meta-cleavage compound hydrolase from Pseudomonas fluorescens IP01

2002

Saku, T.; Fushinobu, S.; Jun, S.; Ikeda, N.; Nojiri, H.; Yamane, H.; Omori, T.; Wakagi, T.

J. Biosci. Bioeng.

93

568-574

Links to other databases for 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate

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Show Links (1 entry)

ChEBI

PubChem

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