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Ligand N-alpha-acetyl-L-lysine

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (11 results)

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Show Substrate (11 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.4.3.20

alpha-N-acetyl-L-lysine + H2O = N-acetyl-6-oxo-L-norleucine + H2O2 + NH3

672432

-

2.6.1.118

N2-acetyl-L-lysine + 2-oxoglutarate = N2-acetyl-L-aminoadipate semialdehyde + L-glutamate

753998

-

3.4.21.50

N-acetyl-L-lysine + H2O = N-acetyl-L-lysine + H2O

683817

-

3.5.1.114

N-acetyl-L-lysine + H2O = acetate + L-lysine

679474

-

3.5.1.14

N-acetyllysine + H2O = lysine + acetate

288861

-

3.5.1.14

N-alpha-acetyl-L-lysine + H2O = acetate + L-lysine

657692, 679474

-

3.5.1.14

2 entries

3.5.1.15

N-acetyllysine + H2O = acetate + lysine

209006

-

3.5.1.16

Nalpha-acetyl-L-lysine + H2O = L-lysine + acetate

171964

-

3.5.1.17

N2-acetyl-L-lysine + H2O = L-lysine + acetate

171966

-

6.1.1.26

ATP + N-acetyl-L-lysine + tRNAPyl = AMP + diphosphate + N-acetyl-L-lysyl-tRNAPyl

727522

-

6.1.1.26

ATP + N-alpha-acetyl-L-lysine + tRNAPyl = ?

690734

-

6.1.1.26

2 entries

Product in Enzyme-catalyzed Reactions (13 results)

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Show Product (13 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.4.3.5

N2-acetyl-N6-(5'-phosphopyridoxyl)-L-lysine + H2O + O2 = pyridoxal 5'-phosphate + N2-acetyl-L-lysine + H2O2

391882

-

3.1.1.29

N-acetyl-Lys-tRNA + H2O = N-acetyl-Lys + tRNA

-

-

3.4.19.12

N6-(N2-acetyl)Lys-ubiquitin + H2O = ubiquitin + N2-acetyl-Lys

36457

-

3.4.21.50

acetyl-Lys-p-nitroaniline + H2O = acetyl-Lys + p-nitroaniline

651374

-

3.4.21.50

N-acetyl-L-lysine + H2O = N-acetyl-L-lysine + H2O

-

-

3.4.21.50

2 entries

3.4.22.14

acetyl-Lys methyl ester + H2O = acetyl-Lys + methanol

-

-

3.4.22.47

acetyl-lysine-4-nitroanilide + H2O = acetyl-lysine + 4-nitroaniline

-

-

3.4.22.47

N-alpha-acetyl-L-lysine-4-nitroanilide + H2O = N-alpha-acetyl-L-lysine + 4-nitroaniline

-

-

3.4.22.47

N-alpha-acetyl-L-lysine-p-nitroanilide + H2O = N-alpha-acetyl-L-lysine + 4-nitroaniline

-

-

3.4.22.47

Nalpha-acetyl-L-lysine 4-nitroanilide + H2O = Nalpha-acetyl-L-lysine + 4-nitroaniline

-

-

3.4.22.47

4 entries

3.4.22.8

Ac-L-Lys-4-nitroanilide + H2O = N2-acetyl-L-Lys + 4-nitroaniline

-

-

3.5.1.124

N6-(1-hydroxy-2-oxopropyl)-N2-acetyl-L-lysine + H2O = N-acetyl-L-lysine + (R)-lactate

-

-

4.3.2.8

alpha-N-acetyl-epsilon-(L-gamma-glutamyl)-L-lysine methyl ester = alpha-N-acetyl-L-lysine + 5-oxo-L-proline

-

-

Inhibitor in Enzyme-catalyzed Reactions (2 results)

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Show Inhibitors (2 entries)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

3.4.17.3

with benzoyl-Gly-Lys as substrate

36405

-

7.4.2.5

-

720986

-

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (3 results)

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Show Turnover Number (3 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

2.6.1.118

0.8

-

pH 8.9, 45°C

753998

6.1.1.26

0.00731

-

pH and temperature not specified in the publication, mutant enzyme L301M/Y306L/C348S/A315V

727522

6.1.1.26

0.0323

-

pH and temperature not specified in the publication, mutant enzyme L301M/Y306L/L309A/C348F

727522

6.1.1.26

2 entries

K_M Value (8 results)

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Show KM Value (8 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.6.1.118

10

-

pH 8.9, 45°C

753998

3.4.21.50

1.59

-

pH 9, rate of carboxyl oxygen exchange reaction by Lys-C is accelerated at acidic pH conditions.

683817

3.5.1.114

1.3

-

pH 7.5, 37°C

679474

3.5.1.14

1.3

-

37°C, pH 7.5

679474

3.5.1.14

6.7

-

pH 7.0, 30°C

657692

3.5.1.14

2 entries

3.5.1.16

4.1

-

-

171964

6.1.1.26

7.8

-

pH and temperature not specified in the publication, mutant enzyme L301M/Y306L/C348S/A315V

727522

6.1.1.26

35.3

-

pH and temperature not specified in the publication, mutant enzyme L301M/Y306L/L309A/C348F

727522

6.1.1.26

2 entries

References & Links

Literature References (16)

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Show Reference (16 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

36405

7061043

Some properties of porcine carboxypeptidase N

1982

Juillerat-Jeanneret, L.; Roth, M.; Bargetzi, J.P.

Hoppe-Seyler's Z. Physiol. Chem.

363

51-58

36457

9521656

Substrate specificity of deubiquitinating enzymes: ubiquitin C-terminal hydrolases

1998

Larsen, C.N.; Krantz, B.A.; Wilkinson, K.D.

Biochemistry

37

3358-3368

171964

10684608

Mechanistic analysis of the argE-encoded N-acetylornithine deacetylase

2000

Javid-Majd, F.; Blanchard, J.S.

Biochemistry

39

1285-1293

171966

5658148

Further studies on epsilon-lysine acylase. The omega-N-acyl-diamino acid hydrolase activity of avian kidney

1968

Leclerc, J.; Benoiton, L.

Can. J. Biochem.

46

471-474

209006

10837925

Murine aspartoacylase: cloning, expression and comparison with the human enzyme

2000

Namboodiri, M.A.A.; Corigliano-Murphy, A.; Jiang, G.; Rollag, M.; Provencio, I.

Mol. Brain Res.

77

285-289

288861

-

Co2+-substituted acylamino acid amido hydrolase from Aspergillus oryzae

1981

Gilles, I.; Loeffler, H.G.; Schneider, F.

Z. Naturforsch. C

36

751-754

391882

6244277

Effects of epsilon-pyridoxyllysine and related compounds on liver and brain pyridoxal kinase and liver pyridoxamine (pyridoxine) 5-phosphate oxidase

1980

Gregory III, J.F.

J. Biol. Chem.

255

2355-2359

651374

12127482

Lysobacter strain with high lysyl endopeptidase production

2002

Chohnan, S.; Nonaka, J.; Teramoto, K.; Taniguchi, K.; Kameda, Y.; Tamura, H.; Kurusu, Y.; Norioka, S.; Masaki, T.; Sakiyama, F.

FEMS Microbiol. Lett.

213

13-20

657692

12682714

Purification and characterisation of a lactococcal aminoacylase

2003

Curley, P.; van der Does, C.; Driessen, A.J.; Kok, J.; van Sinderen, D.

Arch. Microbiol.

179

402-408

672432

17030025

A novel type of lysine oxidase: L-lysine-epsilon-oxidase

2006

Gomez, D.; Lucas-Elio, P.; Sanchez-Amat, A.; Solano, F.

Biochim. Biophys. Acta

247; 1764

1577-1585

679474

17012540

Specificity of aminoacylase III-mediated deacetylation of mercapturic acids

2007

Newman, D.; Abuladze, N.; Scholz, K.; Dekant, W.; Tsuprun, V.; Ryazantsev, S.; Bondar, G.; Sassani, P.; Kurtz, I.; Pushkin, A.

Drug Metab. Dispos.

35

43-50

683817

16823974

pH dependency of the carboxyl oxygen exchange reaction catalyzed by lysyl endopeptidase and trypsin

2006

Hajkova, D.; Rao, K.C.; Miyagi, M.

J. Proteome Res.

5

1667-1673

690734

18471995

Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases

2008

Mukai, T.; Kobayashi, T.; Hino, N.; Yanagisawa, T.; Sakamoto, K.; Yokoyama, S.

Biochem. Biophys. Res. Commun.

371

818-822

720986

21933132

Functional investigation of conserved membrane-embedded glutamate residues in the proton-coupled peptide transporter YjdL

2012

Jensen, J.; Ernst, H.; Wang, X.; Hald, H.; Ditta, A.; Ismat, F.; Rahman, M.; Mirza, O.

Protein Pept. Lett.

19

282-287

727522

22289181

N-Acetyl lysyl-tRNA synthetases evolved by a CcdB-based selection possess N-acetyl lysine specificity in vitro and in vivo

2012

Umehara, T.; Kim, J.; Lee, S.; Guo, L.T.; Söll, D.; Park, H.S.

FEBS Lett.

586

729-733

753998

11489859

Functional and evolutionary relationship between arginine biosynthesis and prokaryotic lysine biosynthesis through alpha-aminoadipate

2001

Miyazaki, J.; Kobashi, N.; Nishiyama, M.; Yamane, H.

J. Bacteriol.

183

5067-5073

Links to other databases for N-alpha-acetyl-L-lysine

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Show Links (1 entry)

ChEBI

PubChem

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