Ligand O2-

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Basic Ligand Information

Molecular Structure
Picture of O2- (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
O2
O2-
MXDZWXWHPVATGF-UHFFFAOYSA-N
Synonyms:
O(2), O2-., O2.-, superoxide, superoxide anion, superoxide anion radical, superoxide ion, superoxide radical


Show all pahtways known for Show all BRENDA pathways known for O2-

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (14 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
L-tryptophan + O2- = N-formyl-L-kynurenine
show the reaction diagram
-
hypoxanthine + NAD+ + H+ + O2- + H2O = xanthine + NADH + H2O2
show the reaction diagram
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a [sulfatase]-L-cysteine + O(2) + a thiol = a [sulfatase]-3-oxo-L-alanine + hydrogen sulfide + a disulfide + H2O
show the reaction diagram
-

In Vivo Product in Enzyme-catalyzed Reactions (3 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
NADPH + O2 = NADP+ + O2-
show the reaction diagram
-

Substrate in Enzyme-catalyzed Reactions (29 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
serotonin + superoxide = tryptamine-4,5-dione + H2O
show the reaction diagram
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hypoxanthine + NAD+ + H+ + O2- + H2O = xanthine + NADH + H2O2
show the reaction diagram
-
O2-. + H+ = H2O2 + O2
show the reaction diagram
-
a [sulfatase]-L-cysteine + O(2) + a thiol = a [sulfatase]-3-oxo-L-alanine + hydrogen sulfide + a disulfide + H2O
show the reaction diagram
-

Product in Enzyme-catalyzed Reactions (11 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
L-lactate + O2 = ? + superoxide anion
show the reaction diagram
-
-
xanthine + H2O + O2 = superoxide + urate + ?
show the reaction diagram
-
-
NADH + O2 = NAD+ + O2-
show the reaction diagram
-
-
NADPH + O2 = NADP+ + O2-
show the reaction diagram
-
NADPH + O2 = NADP+ + O2-
show the reaction diagram
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paraquat radical + O2 = paraquat + O2-
show the reaction diagram
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-
NADH + O2 = NAD+ + superoxide radical
show the reaction diagram
-

Activator in Enzyme-catalyzed Reactions (4 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
stimulation of O2 uptake
-
activation of the enzyme is stimulated by superoxide and H2O2
-

Inhibitor in Enzyme-catalyzed Reactions (12 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
substrate inhibition for mutant C140S/Q143A
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superoxide generated in the chloroplast stroma causes photoinhibition of photosystem I, superoxide accelerates photoinhibition of PSI
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generated by a Fe3+/ascorbate system, inhibits cytosolic GSTs and microsomal GSTs by 30% and 65%, respectively. Addition of 2 mM DTT fails to completely reverse microsomal GST inhibition elicited by Fe3+/ascorbate, recovering only about 10% of this activity, but it completely reverses the inhibitory effect of Fe3+/ascorbate on cytosolic GST activity
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displaces the iron atom from the enzyme, only the Fe2+-metalloform of the enzyme can be inactivated by hydrogen peroxide or superoxide. Superoxide stress promotes the mismetallation of DAHP synthase
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superoxide production decreases the cellular redox potential and specifically inhibites caspase-1 by reversible oxidation and glutathionylation of the redox-sensitive cysteine residues Cys397 and Cys362
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sensitivity of glyoxalase III is special and might relate to the thiol group that is essential for its activity and possibly to the binding of iron adjacent to the active site thiol
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Enzyme Kinetic Parameters

kcat Value (Turnover Number) (6 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE

KM Value (2 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE

Ki Value (1 result)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.06
-
mutant C140S/Q143A, pH 9.0, 25°C

References & Links

Links to other databases for O2-

ChEBI
PubChem
ChEBI
PubChem