Ligand nitrate

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Basic Ligand Information

Molecular Structure
Picture of nitrate (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
NO3
nitrate
NHNBFGGVMKEFGY-UHFFFAOYSA-N
Synonyms:
nitrate[side 2], NO3-


Show all pahtways known for Show all BRENDA pathways known for nitrate

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (20 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
nitrate + NADH = nitrite + NAD+ + H2O
show the reaction diagram
-
4-cresol + nitrate + H2O = 4-hydroxybenzaldehyde + ?
show the reaction diagram
-
nitrate + reduced acceptor = nitrite + acceptor
show the reaction diagram
-
NAD(P)H + nitrate = ?
show the reaction diagram
-
nitrate + quinol = nitrite + quinone + H2O
show the reaction diagram
-

In Vivo Product in Enzyme-catalyzed Reactions (12 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
3 propionate-3-nitronate + O2 = 3 malonic semialdehyde + nitrite + 2 nitrate + H2O2
show the reaction diagram
-
-
3 nitrite + 2 H+ = 2 nitric oxide + nitrate + H2O
show the reaction diagram
-
-

Substrate in Enzyme-catalyzed Reactions (70 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
4-cresol + nitrate + H2O = 4-hydroxybenzaldehyde + ?
show the reaction diagram
-
nitrate + reduced electron acceptor = nitrite + H2O + oxidized electron acceptor
show the reaction diagram
-

Product in Enzyme-catalyzed Reactions (26 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
3 propionate-3-nitronate + O2 = 3 malonic semialdehyde + nitrite + 2 nitrate + H2O2
show the reaction diagram
-
-
Ngamma-hydroxy-L-arginine + H2O2 = citrulline + Ndelta-cyanoornithine + NO2- + NO3-
show the reaction diagram
-
3 nitrite + 2 H+ = 2 nitric oxide + nitrate + H2O
show the reaction diagram
-
-
nitrite + acceptor = nitrate + reduced acceptor
show the reaction diagram
-
-
nitric oxide + NADH = nitrate + NAD+
show the reaction diagram
-
-
nitrite + methyl viologen = nitrate + oxidized methyl viologen
show the reaction diagram
-

Activator in Enzyme-catalyzed Reactions (16 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
stimulates
-
2fold enhanced induction independently of the NH4+ concentration, growth stimulation is higher on nitrate than on nitrite
-
50% activation at 3-5 mM, exogenous nitrate at 14.2 mM absorbed in the form of KNO3 and Ca(NO3)2 during 10-20 days exponentially activates the enzyme in the roots by 22-100% as compared with plants grown on nitrogen-free medium. Under low light, nitrate can not activate sucrose synthase
-
stimulation at low concentrations, inhibition above 15 mM
-
50 mM 1.6fold activation of cytosolic PFK
-
50 mM, activates
-
activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate
-
exposure to 0.2 mM NO3- brings about an increase in ATP hydrolytic activity after 8 h (31%) and 24 h (33%) compared to the control
-

Inhibitor in Enzyme-catalyzed Reactions (165 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
100 mM
-
100 mM, 87% inhibition of D-glycerate oxidation
-
pH 5.5: competitive inhibition of the chlorination reaction with respect to chloride, uncompetitive with respect to H2O2
-
45% inactivation at 0.1 M
-
50% inhibition at 0.035 M
-
39% inhibition at 1 mM
-
2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide suppresses the inhibition by nitrate
-
uncompetitive inhibitor with NAD+, dead end inhibitor
-
moderate inhibitor
-
mM concentration
-
weak inhibition, inhibition mode, overview
37% electrochemical activity at 100 mM inhibitor concentration, at 3 mM 50% inhibition
-
at high substrate concentration, substrate inhibition occurs, mechanism of substrate inhibition in Nap, and kinetics, overview
inhibition above 150 mM, stimulation below
-
not at 5 mM, only at 10 mM
-
10 mM, 9% inhibition
-
slight inhibition
-
inhibits the nezyme, overview
-
nitrate completely inhibits atrazine degradation activity in strain KU001
-
inhibitory at 20 mM
-
12 mM for 50% inhibition
100-200 mM
-
10 mM, 20% decrease of activity
-
noncompetitive
-
0.7 M, 50% inhibition of ATP-diphosphate exchange
-
1.0 M, 50% inhibition of ATP-diphosphate exchange
-

Metals and Ions (15 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
activates plastidic isozymes by 10fold by direct action on the enzyme
-
stimulates
-
activates
-
18% enhanced activity at 0.8 mM
-
2 mM , activation
-
stimulation by anions with the following decreasing order of effectiveness: SCN-, I-, NO3-, Br-, Cl-, membrane-associated enzyme. The effect of NO3- is maximal at pH 7-8, it is negligible at pH 6.5
-
stimulates Asn synthesis and Gln hydrolysis
-

3D Structure of Enzyme-Ligand-Complex (PDB) (365 results)

EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (7 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE

KM Value (61 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE

Ki Value (25 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.4
-
-
1.6
-
wild type enzyme, at pH 9.0 and 25°C
8
-
with L-arginine as substrate
37
-
-

IC50 Value (5 results)

EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE
8
-
pH 6.0, 70°C

References & Links

Links to other databases for nitrate

ChEBI
PubChem
ChEBI
PubChem