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Ligand 1-fluoro-2,4-dinitrobenzene

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Basic Ligand Information

Molecular Structure
Picture of 1-fluoro-2,4-dinitrobenzene (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C6H3FN2O4
1-fluoro-2,4-dinitrobenzene
LOTKRQAVGJMPNV-UHFFFAOYSA-N
Synonyms:
2,4-dinitro-1-fluorbenzene, 2,4-Dinitro-1-fluorobenzene, 2,4-dinitrofluorobenzene, Fluorodinitrobenzene

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
1-fluoro-2,4-dinitrobenzene + glutathione = ?
show the reaction diagram
-

Substrate in Enzyme-catalyzed Reactions (3 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
1-fluoro-2,4-dinitrobenzene + glutathione = ?
show the reaction diagram
-

Activator in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
up to 90% inhibition at pH 6.5-7.0, slight increase at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor
-

Inhibitor in Enzyme-catalyzed Reactions (31 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
0.1 mM, 71% inhibition
-
after 2 h at 2 mM, complete inactivation by dinitrophenylation of 2 mol of histidine residues per mol of enzyme-bound FMN, competitive inhibitors such as phosphate, nitrate and alpha-hydroxymalonate decrease the rate of inactivation
-
i.e. Sanger's reagent
-
11% residual activity at 2.5 mM
-
reversible
-
irreversible, with NADPH, alkylation of the active site cysteine disulfide, strong increase in oxidation activity of the enzyme against NADPH
-
1 mM, 2% activity
-
up to 90% inhibition at pH 6.5-7.0, slight increase at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor
-
6 mM
-
rate of inhibition is accelerated by 1 mM ascorbic acid
-
5 mM, no inactivation after 30 min and 15% inactivation after 120 min in Tris-HCl, pH 8.0
-

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (6 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE

KM Value (5 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE

References & Links

Links to other databases for 1-fluoro-2,4-dinitrobenzene

ChEBI
PubChem
ChEBI
PubChem