Ligand diisopropyl fluorophosphate

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Basic Ligand Information

Molecular Structure
Picture of diisopropyl fluorophosphate (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C6H14FO3P
diisopropyl fluorophosphate
RQASLOJQHHELIQ-QJSKOQLASA-N
Synonyms:
bis(1-methylethyl) fluorophosphate, DFP, di-isopropyl-phosphofluoridate, di-isopropylfluorophosphate, di-isopropyl fluorophosphate, di-isopropylphosphofluoridate, diisopropylfluorophosphate, diisopropyl fluorophosphates, diisopropyl fluorophosphonate, diisopropylfluorphosphate, diisopropyl flurophosphate, diisopropyl phosphofluoridate, diisopropylphosphofluoridate, diisopropyl phosphofluoride, diisopropylphosphofluoride, diisopropylphosphorofluoridate, diisopropyl phosphorofluoridate, diisopropylphosphoryl fluoride, dipropan-2-yl phosphorofluoridate, Isoflurophate, O,O-diisopropyl phosphofluoridate

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (2 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE

Substrate in Enzyme-catalyzed Reactions (9 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
diisopropyl phosphofluoridate + H2O = ?
show the reaction diagram
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Activator in Enzyme-catalyzed Reactions (4 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
1.0 mM, activation to 116.3% of control
-
weak activation
-
1 mM, 15% inhibition
-
17% activation at 1 mM
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Inhibitor in Enzyme-catalyzed Reactions (431 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
non-competitive
-
partial
-
complete inhibition of both enzyme activities at 20 mM
-
weak inhibition
-
to some extent
-
strong inhibitor, 50% inhibition at 0.1 mM
-
5 mM, 40% inhibition
-
0.3 mM, complete inhibition
-
0.1 mM, no residual activity
-
1 mM, complete inhibition
-
complete inhibition at 0.01 mM
-
complete inhibition at 1 mM, 80% inhibition at 0.1 mM
-
9% relative residual activity
-
the recombinant enzyme shows about 63% residual activity at 1 mM
-
bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase
-
IC50: 0.8 for 65 kDa BPP hydrolase
-
1 mM, 34% inhibition of the membrane-bound enzyme
-
1 mM, 18% inhibition
-
42% inhibition at 10 mM
-
1 mM, 48% inhibition
-
16% inhibition at 1 mM at pH 7.4
-
pretreatment, complete inhibition
-
strongly inhibitory at 1 mM
-
5 mM, 25% inhibition. 10 mM, 44% inhibition
-
87% inhibition at 1 mM
-
alone slightly and in combination with p-hydroxymercuribenzoate strong
-
1 mM, 28% inhibition
-
irreversible inhibition; mechansim-based inhibitor
-
complete inhibition at 5 mM
-
complete inhibition at 4 mM
-
complete inhibition at 5 mM
-
strongly inhibits
-
2 mM, 35% inhibition
-
0.05 mM, 37% inhibition. Rapid and irreverible
-
inhibits cleavage of synthetic tetradecapeptide renin substrate, irreversible inhibition of benzoyl-arginine 4-nitroanilide
-
inhibits esterase activity
-
causes total inhibition
-
partial inhibition
-
87% inhibition at 2 mM
-
complete inhibition at 0.1 mM
-
binds covalently to the enzyme
-
0.5 mM, complete inhibition
-
1 mM, 88% inhibition
-
0.01 mM, no residual activity
-
strong
-
gamma-renin only
-
1.0 mM, 30 min, 20°C, activity of protease I is reduced by 10%, activity of protease II by 30%
-
1 mM, weak inhibition at pH 5.5 and pH 8.0 with and without addition of 1 mM Co2+
-
1 mM, 81% inhibition
-
10 mM, about 70% inhibition
-
at 0.5 mM, 15.5% inhibition
-
strain PS-21 not inhibited
-
the substrate protects against inhibition
-
weak inhibition, Ki: 33 mM
-
5 mM, 18% inhibition
-
complete inhibition at 0.01 mM
-
2 mM, 90% inhibition
-
1 mM, 30% inhibition
-
0.3 mM
-
1 mM
-
2 mM, 80% inhibition
-
10 mM, 25% inhibition
-
0.05 mM, 100% inhibition
-

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (42 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
41
-
-

KM Value (52 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE

Ki Value (7 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.006
-
pH 7.5, 25°C
0.44
-
pH 8.5, 25°C, wild-type enzyme, substrate paraoxon
0.00000007
-
pH 7.2, 25°C
33
-
weak inhibition

IC50 Value (16 results)

EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE
0.00196
-
-
0.045
-
i.e. DFP, n-C8H17P(O)(OCH3)F, IC50 is 0.045 mM
8
-
IC50: 8 mM
0.8
-
IC50: 0.8 mM for 65 kDa BPP hydrolase
1.15
-
IC50: 1.15 mM
0.00005
-
IC50: 50 nM
0.01
-
-

References & Links

Links to other databases for diisopropyl fluorophosphate

ChEBI
PubChem
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PubChem