Ligand DNA

YabT possesses a DNA-binding motif essential for its activation: in the cytosolic part of YabT, partially overlapping with the catalytic domain, there is a region rich in lysine and arginine residues stretching from residues 230-315, which forms the DNA binding site. The entire region of amino acid residues 230-315 is required for DNA binding. YabT is capable of binding both ssDNA and dsDNA, binding of ssDNA seems to be more efficient. YabT binding of DNA is not sequence specific, and can be achieved with DNA fragments with minimal length of 15 bases. Also the activation of YabT is more efficient with ssDNA

743306

2.7.11.23

activation

640980

2.7.11.7

autophosphorylation activity is increased 5-10fold in a Ca2+-independent manner

640777

2.7.7.86

the cyclase activity is stimulated by DNA but not by RNA

ATPase activity is activated by primed DNA templates, such as poly(dA)-oligo(dT). ATPase activity of the SsoRFC-complex is substantially stimulated by the primed homopolymer, whereas no effect is detected in the presence of poly(dA)400. The maximal activation (about tenfold) of the ATP-hydrolyzing activity is measured in the presence of poly(dA)4000-oligo(dT)45 at 140 nM

stimulates

stability of the enzyme's closed clamp conformation may influence DNA-stimulated ATP hydrolysis

Inhibitor in Enzyme-catalyzed Reactions (27 results)

various DNA lesions significantly affect the efficiency and fidelity of RNA synthesis. DNA modifications that disrupt correct base-pairing can strongly inhibit transcription and increase nucleotide misincorporation by RNAP. The universal transcription factor GreA and Deinococcus-specific factor Gfh1 stimulate RNAP stalling at various DNA lesions, depending on the type of the lesion and the presence of Mn2+ ions

762379

2.7.99.B1

the enzyme activity is inhibited by DNA-binding

cleavage of DNA is inhibited by an increased degree of negative supercoiling

134241

3.1.22.1

inhibitory above 0.4 mg/ml

134308

3.1.26.4

single-stranded or double-stranded, very strong

markedly inhibited by low concentrations of native DNA

171777

3.2.2.17

depurinated DNA competitively inhibits the UV endonuclease activity, irradiated DNA inhibits the AP endonuclease activity

strong inhibition by denaturated DNA, slight inhibition by native DNA

209949

4.6.1.19

denatured

134496

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (21 results)

0.117

0.4

pH 7.6, 21°C, C/G matched nick DNA substrate

661248

K_M Value (51 results)

0.0000018

5'-OH blunt end double-stranded DNA

0.0142

0.000136

pH 7.6, 21°C, C/G matched nick DNA substrate

661248

References & Links

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Show Reference (736 entries)

Links to other databases for DNA

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Ligand DNA

Basic Ligand Information

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (35 results)

In Vivo Product in Enzyme-catalyzed Reactions (16 results)

Substrate in Enzyme-catalyzed Reactions (90 results)

Product in Enzyme-catalyzed Reactions (71 results)

Activator in Enzyme-catalyzed Reactions (28 results)

Inhibitor in Enzyme-catalyzed Reactions (27 results)

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (21 results)

KM Value (51 results)

References & Links

Literature References (736)

Links to other databases for DNA

k_cat Value (Turnover Number) (21 results)

K_M Value (51 results)