Ligand oxaloacetic acid

4.1.1.31

phosphate + oxaloacetate = phosphoenolpyruvate + HCO3-

728484, 728519, 727178, 727209, 727236, 727533

keto-Oxaloacetate = Enol-oxaloacetate

771053

4 entries

In Vivo Product in Enzyme-catalyzed Reactions (92 results)

top print hide

1.1.1.345

D-malate + NAD+ = oxaloacetate + NADH + H+

1.1.1.37

25 entries

1.1.1.375

(S)-malate + NAD+ = oxaloacetate + NADH + H+

1.1.1.40

Pyruvate + CO2 = Oxaloacetate

1.1.1.82

(S)-malate + NADP+ = oxaloacetate + NADPH + H+

D-aspartic acid + H2O + O2 = oxaloacetate + NH3 + H2O2

1.4.3.16

2 entries

1.4.3.2

L-Asp + H2O + O2 = oxaloacetate + NH3 + H2O2

1.4.3.3

2 entries

1.4.3.7

D-aspartate + H2O + O2 = oxaloacetate + NH3 + H2O2

395288, 395287, 395286

2.1.3.1

(S)-methylmalonyl-CoA + pyruvate = propanoyl-CoA + oxaloacetate

L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate

639822, 639824, 639840, 639856, 639865, 639875, 639859, 639872, 639815, 639823, 639844, 639868, 639886, 639816, 639817, 639821, 639831, 639850, 639830, 639834, 639828, 639845, 639836, 639851, 639832, 286649, 639862, 639838, 639849, 639854, 639820, 639861, 636732, 636660, 639883, 639833, 639878, 639881, 639871, 639873, 636643, 639841, 639842, 639843, 639855, 639870, 639879, 639884, 639869, 639829, 639880, 639839, 639864, 639876, 639814, 639885, 639846, 639818, 639819, 639837, 639863, 639835, 639867, 636718, 636719, 639874, 639860, 639858, 636716, 639826, 639825, 639882, 639866, 639848, -

2.6.1.21

D-aspartate + 2-oxoglutarate = 2-oxosuccinate + D-glutamate

2.6.1.35

glyoxylate + L-aspartate = glycine + oxaloacetate

639968, 639967, 639966

2.6.1.57

L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate

4RKD, 3D-view

2.6.1.70

L-aspartate + 3-phenylpyruvate = oxaloacetate + L-phenylalanine

640191

2.6.1.78

prephenate + L-aspartate = L-arogenate + oxaloacetate

phosphate + phosphoenolpyruvate + CO2 = diphosphate + oxaloacetate

citrate = acetate + oxaloacetate

441599, 492186, 653146, 649579, -

1Z6K, 3D-view

4.1.3.B3

4-carboxy-4-hydroxy-2-oxoadipate = oxaloacetate + pyruvate

4.2.1.32

L-tartrate = oxaloacetate + H2O

4.2.1.81

(S,S)-tartrate = oxaloacetate + H2O

648404, 648403

4.3.1.16

L-threo-3-hydroxyaspartate = oxaloacetate + NH3

Substrate in Enzyme-catalyzed Reactions (375 results)

oxalacetic acid + NADPH = ? + NADP+

655067

1.1.1.27

oxaloacetate + NADH + H+ = ? + NAD+

oxaloacetic acid + NAD+ = ?

oxaloacetate + NADH + H+ = D-malate + NAD+

oxaloacetate + NADH + H+ = ?

764063

1.1.1.79

oxaloacetate + NADPH = malate + NADP+

287328, 287321

1.1.1.81

oxaloacetate + NAD(P)H = malate + NAD(P)+

D-2-hydroxyglutarate + oxaloacetate = 2-oxoglutarate + malate

740757

1.1.99.7

(S)-lactate + oxaloacetate = malate + pyruvate

287848, 287849, 287846, 287847, 287844, 287850, 287845

1.2.1.51

oxaloacetate + CoA + NADP+ = ? + CO2 + NADPH

oxaloacetate + H2O + methyl viologen = ?

methylamine + oxaloacetate + NADPH = aspartic acid + H2O + NADP+

391526

1.4.1.2

oxaloacetate + NADPH + NH3 = L-aspartate + NADP+ + H2O

oxaloacetate + NH3 + NADH + H+ = L-aspartate + H2O + NAD+

391632

1.5.1.11

L-Arg + oxaloacetate + NADH = ?

13301, 13317, 13318, 13323, 13302

1.5.1.17

L-Ala + oxaloacetate + NADH = ?

13374, 13373, 13369

1.5.1.22

1.5.1.23

taurine + oxaloacetate + NADH = ?

13440, 13439, 13437

1.5.1.24

L-ornithine + oxaloacetic acid + NADPH + H+ = ? + NADP+ + H2O

712294

1.5.1.28

L-Met + oxaloacetate + NADH = ?

94167

1.5.1.52

(2S)-2-amino-4-[[(1R)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino]butanoate + oxaloacetate + NADPH + H+ = ? + NADP+ + H2O

(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ = ? + NADP+ + H2O

acetyl-CoA + H2O + oxalacetic acid = ? + CoA

(S)-1-phenylethylamine + oxaloacetate = acetophenone + ?

756287

2.6.1.12

L-alanine + oxaloacetate = pyruvate + L-aspartate

636593

2.6.1.120

beta-alanine + oxaloacetate = 3-oxopropanoate + ?

763265

2.6.1.13

ornithine + oxaloacetate = DELTA1-pyrroline-5-carboxylate + L-aspartate

637103, 637110, 637107, 637111, 637130

2.6.1.15

L-glutamine + oxaloacetate = 2-oxoglutaramate + L-aspartate

746952

2.6.1.18

beta-alanine + oxaloacetic acid = malonic semialdehyde + L-aspartate

636596

2.6.1.23

4-hydroxy-L-glutamate + oxaloacetate = 4-hydroxy-2-oxoglutarate + L-aspartate

639934, 639935

2.6.1.24

3,5-diiodo-L-tyrosine + oxaloacetate = 3,5-diiodo-4-hydroxyphenylpyruvate + L-aspartate

639936

2.6.1.26

L-3,5,3'-triiodothyronine + oxaloacetate = 3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + glycine

pyridoxamine + oxaloacetate = pyridoxal + L-aspartate

639961, 639962

2.6.1.36

L-lysine + oxaloacetate = 2-aminoadipate 6-semialdehyde + L-aspartate

637024, 637018, 637023, 637031

2.6.1.39

oxaloacetate + L-glutamate = 2-aminosuccinate + 2-oxoglutarate

659885

2.6.1.40

(R)-3-amino-2-methylpropanoate + oxaloacetate = 2-methyl-3-oxopropanoate + L-aspartate

644426, 644430, 636964

2.6.1.41

D-methionine + oxaloacetate = 4-methylthio-2-oxobutanoate + L-aspartate

3-(3,4-dihydroxyphenyl)-L-alanine + oxaloacetic acid = 3-(3,4-dihydroxyphenyl)pyruvate + L-aspartate

L-serine + oxaloacetate = 3-hydroxypyruvate + L-aspartate

L-leucine + oxaloacetate = 4-methyl-2-oxopentanoate + L-aspartate

639996

2.6.1.64

L-glutamine + oxaloacetate = 2-oxoglutaramate + L-aspartate

639901, 640171, 640172

L-methionine + oxaloacetate = 4-methylsulfanyl-2-oxobutanoate + L-aspartate

636989, 636990

2.6.1.76

L-lysine + oxaloacetic acid = ?

288731

2.6.1.77

taurine + oxaloacetate = ?

L-histidinol phosphate + oxaloacetate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-aspartate

637151

2.6.1.B16

(S)-1-phenylethylamine + oxaloacetate = acetophenone + L-aspartate

758672

2.6.1.B3

(2S,3S,5R,10S,12R,14R,15S,16S)-2-amino-12,16-dimethylicosane-3,5,10,14,15-pentol + oxaloacetate = (3S,5R,10S,12R,14R,15S,16S)-3,5,10,14,15-pentahydroxy-12,16-dimethylicosan-2-one + L-aspartate

724058

2.8.3.1

acetyl-CoA + oxaloacetate = acetate + oxaloacetyl-CoA

33928

2.8.3.18

succinyl-CoA + oxaloacetate = oxaloacetyl-CoA + succinate

721675

3.7.1.1

oxaloacetate + H2O = oxalate + acetate

210614, 210610, 210612, 210609, 210608, 668652, 670169, 670718, 687682, 686268, 693684, 689936, 719546, 719879, 734415, 734735, 735295, 757260, 776500, 776843

3.7.1.2

oxaloacetate + H2O = ?

289242

3.7.1.5

Oxaloacetate = Pyruvate + CO2

oxaloacetate = ?

oxaloacetate + NAD+ = ?

648380

4.1.3.1

oxalacetate = oxalate + acetate

664083

4.1.3.13

Oxaloacetate + glyoxylate = 3-Oxalomalate

5222

4.1.3.16

Oxaloacetate = CO2 + pyruvate

5243, 5241, 5255, 5252

Oxaloacetate = CO2 + pyruvate

acetate + oxaloacetate = citrate

33352, 33363

1Z6K, 3D-view

5.3.2.2

keto-Oxaloacetate = Enol-oxaloacetate

2875, 2874, 2878, 2880, 2877, 2879, 2876, 771053

6.2.1.9

ATP + oxaloacetate + CoA = ADP + phosphate + oxaloacetyl-CoA

743968

ADP + phosphate + oxaloacetate = ATP + pyruvate + HCO3-

774663

4 entries

Product in Enzyme-catalyzed Reactions (296 results)

D-malate + NAD+ = oxaloacetate + NADH + H+

Pyruvate + CO2 = Oxaloacetate

1.1.1.82

1.1.1.85

malate + NAD+ = 3-oxosuccinate + NADH + H+

1.1.5.4

18 entries

1.1.99.14

DL-malate + acceptor = 2-oxosuccinate + reduced acceptor

1.1.99.6

D-malate + acceptor = oxaloacetate + reduced acceptor

287840

1.1.99.7

pyruvate + L-malate = (S)-lactate + oxaloacetate

1.13.12.4

(S)-malate + O2 = oxaloacetate + H2O2

L-Asp + H2O + NAD+ = oxaloacetate + NH3 + NADH

D-aspartate + oxidized 2,6-dichloroindophenol + H2O = oxaloacetate + NH3 + reduced 2,6-dichloroindophenol

2.1.3.1

(S)-methylmalonyl-CoA + pyruvate = propanoyl-CoA + oxaloacetate

pyruvate + L-aspartate = L-alanine + oxaloacetate

L-aspartate + 2-oxoglutarate = 2-oxobutan-1,4-dioate + L-glutamate

glyoxylate + L-aspartate = glycine + oxaloacetate

639968, 639967, 639966

2.6.1.42

2.6.1.43

L-aspartate + 4,5-dioxopentanoate = 5-aminolevulinate + oxaloacetate

2.6.1.44

L-aspartate + glyoxylate = 2-oxosuccinate + glycine

640068

2.6.1.49

3-(3,4-dihydroxyphenyl)pyruvate + L-aspartate = 3,4-dihydroxy-L-phenylalanine + oxaloacetate

L-aspartate + pyruvate = oxaloacetate + L-alanine

2.6.1.67

L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate

640188, -

2.6.1.70

2.6.1.74

D-aspartate + 2-oxoadipate = oxaloacetate + 2-aminoadipate

2.6.1.75

L-aspartic acid + 2-oxoglutarate = oxaloacetate + L-glutamate

oxaloacetate 4-methyl ester = oxaloacetate + methanol

Pyruvate + CO2 = Oxaloacetate

citrate = acetate + oxaloacetate

33363, 33352, 33349, 33364, 441599, 693, 33358, 33362, 33354, 33360, 698, 33357, 33346, 492186, 33350, 33351, 33353, 33356, 33361, 33367, 33369, 33370, 33372, 33368, 653146, 649579, -

butynedioyl-CoA = oxaloacetate + CoA

(2S,3S)-tartrate = oxaloacetate + H2O

4.2.1.81

(S,S)-tartrate = oxaloacetate + H2O

648404, 648403, -

4.3.1.16

4.3.1.17

L-threo-3-hydroxyaspartate = oxaloacetate + NH3

L-aspartate + pyridoxal 5'-phosphate = 2-oxo-succinate + pyridoxamine 5'-phosphate

649945

6.3.1.5

ATP + deamido-NAD+ + Asp = AMP + diphosphate + NAD+ + oxaloacetate

4 entries

Pyruvate + CO2 = Oxaloacetate

4260, 4279

Activator in Enzyme-catalyzed Reactions (14 results)

stimulates by chelating and stabilizing Mn3+

439830

1.2.1.79

about 15% activation at 5 mM

762707

1.4.7.1

up to 50% increase in activity

activation

50 mM, increasing enzyme activity by 30%

0.030 mM

Inhibitor in Enzyme-catalyzed Reactions (336 results)

inhibition at 5 mM

above 0.3 mM

10 mM, 38.5% inhibition

286830, 286840, 286885, 286904

1.1.1.81

2.5 mM, 10% inhibition of NADH linked hydroxypyruvate reduction, 20% of NADPH linked reduction

competitive

competitive

competitive to acceptor

10 mM, 50% inhibition, noncompetitive

395375

1.13.12.2

67% inhibition at 5 mM

2-oxoglutarate dehydrogenase complex

weak inhibition, competitive with 2-oxoglutarate

at 6.67 mM 90% inhibition

391727

1.4.3.16

weak inhibition; weak inhibition

slight substrate inhibition

the METTL12-mediated methylation is blocked by the citrate synthase substrate oxaloacetate

1 mM, 68% inhibition

competitive to 2-oxoglutarate

in complex with Mg2+, inhibition at low substrate concentrations, inhibition of the translocase component of the glucose 6-phosphatase system

134955

3.2.1.62

50% inhibition of the tartaric acid-stimualted phlorizin hydrolase at 20 mM

a competitive inhibitor

strong inhibition, 8 mM: 59% of activity compared to H2O

1 mM, 90% inhibition

competitive inhibition, 0.2-1 mM

about 68% residual activity at 25 mM

747628

4.1.1.31

4285, 4301, 4305, 4307, 4325, 4332

at 7 mM

competitive, Ki: 0.22 mM

competitive

irreversible

Ki: 8.22 mM

competitive

non-competitive inhibition, pyruvate as varied substrate

34848, 34850, 34854

2 mM, 56% residual activity

661410

5.3.1.9

10 mM, 25% inhibition

20 mM, 40% inhibition

3D Structure of Enzyme-Ligand-Complex (PDB) (799 results)

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (238 results)

1.1

in 50 mM Tris-HCl (pH 8.0) with 50 mM NaCl, 1 mM dithiothreitol, at 22°C

724295

1.1.1.299

444

at pH 7.0 and 70°C

pH 8.6, 37°C

113

pH 8.5, 82°C

0.26

pH 8.0, 22°C

748236

1.5.1.B8

0.29

recombinant enzyme, with NADPH and L-histidine nicotianamine, pH 8.0, 22°C

0.967

500

pH 7.3, 37°C

3.58

enzyme form OAT-1, 25°C, pH 9.0

2877

18 entries

K_M Value (624 results)

0.086

in 50 mM Tris-HCl (pH 8.0) with 50 mM NaCl, 1 mM dithiothreitol, at 22°C

0.05

0.264

pH 8.6, 37°C

0.9

4.9

3.5

L-Met and NADH as cosubstrate

94167

1.5.1.52

1.4

pH 8.0, 22°C

748236

1.5.1.B8

1.9

recombinant enzyme, with NADPH and L-histidine nicotianamine, pH 8.0, 22°C

0.063

0.13

pH 7.5, 30°C

0.177

4.15

pH 8.0, 35°C

0.13

pH 7.5, 25°C

664083

4.1.3.16

condensation with glyoxylate

0.068

enzyme form OAT-1

2877, 2879

0.15

4260, 4266, 4267

K_i Value (65 results)

0.24

at pH 7.0 and 70°C

0.36

pH 7.2, 30°C, versus (S)-malate

711524

1.1.1.40

0.08

at pH 7.5 and 33°C

737473

1.1.1.42

0.35

recombinant enzyme, pH 7.0

0.01

0.5

competitive inhibition with respect to 2-oxoglutarate and noncompetitive with respect to Fe2+ and the peptide substrate

6.4

at pH 7.0

0.0007

pH 7.2

0.4

0.8

competitive inhibitor

0.18

1.5

0.22

competitive

8.22

0.313

pH 7.2, 30°C

769198