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Ligand coumarin
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Basic Ligand Information
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open all tablesRoles as Enzyme Ligand
Substrate in Enzyme-catalyzed Reactions (1 result)
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EC NUMBER 
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
Product in Enzyme-catalyzed Reactions (2 results)
EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
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7-ethoxycoumarin + [reduced NADPH-hemoprotein reductase] + H+ = coumarin + [oxidized NADPH-hemoprotein reductase] + ?
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7-methoxycoumarin + [reduced NADPH-hemoprotein reductase] + H+ = coumarin + [oxidized NADPH-hemoprotein reductase] + ?
Activator in Enzyme-catalyzed Reactions (2 results)
COMMENTARY
EC NUMBER
LITERATURE
ENZYME 3D STRUCTURE
hydroxylase activity is amplifiable by coumarin, increases 50% above that found constitutively
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when coumarin and NO3- are concurrently added to the nutrient solution, a significant increase in the PM H+-ATPase activity is observed. However, coumarin does not affect enzyme activity by direct contact
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Inhibitor in Enzyme-catalyzed Reactions (14 results)
COMMENTARY
EC NUMBER
LITERATURE
ENZYME 3D STRUCTURE
reversible time-independent inhibition. Only dicoumarol, 4-hydroxycoumarin and warfarin inhibit in micromolar ranges. 7-Hydroxy-4-methylcoumarin is ineffective. Competitive inhibition with respect to 2,6-dichlorophenolindophenol, uncompetitive with respect to NADPH. Phenolic hydroxyl group at the C-4 position in the coumarin skeleton is important for the maximal inhibition. Sequence of potency for the inhibitors in descending order: dicoumarol, 4-hydroxycoumarin, warfarin, coumarin
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3D Structure of Enzyme-Ligand-Complex (PDB) (22 results)
EC NUMBER
ENZYME 3D STRUCTURE
Enzyme Kinetic Parameters
Ki Value (20 results)
COMMENTARY
EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
LITERATURE
inhibition of isozyme hCA I, pH not specified in the publication, temperature not specified in the publication
0.0001
-
inhibition of isozyme hCA XII, pH not specified in the publication, temperature not specified in the publication
0.0031
-
inhibition of isozyme hCA IX, pH not specified in the publication, temperature not specified in the publication
0.0092
-
inhibition of isozyme hCA II, pH not specified in the publication, temperature not specified in the publication
0.0001
-
IC50 above 0.5 mM, isoform CA XIV, in 20 m M HEPES (pH 7.5), and 20 mM Na2SO4, at 22°C
0.5
-
IC50 above 0.5 mM, isoform CA XII, in 20 m M HEPES (pH 7.5), and 20 mM Na2SO4, at 22°C
0.5
-
IC50 above 0.5 mM, isoform CA VII, in 20 m M HEPES (pH 7.5), and 20 mM Na2SO4, at 22°C
0.5
-
IC50 above 0.5 mM, isoform CA VI, in 20 m M HEPES (pH 7.5), and 20 mM Na2SO4, at 22°C
0.5
-
IC50 above 0.5 mM, isoform CA VB, in 20 m M HEPES (pH 7.5), and 20 mM Na2SO4, at 22°C
0.5
-
IC50 above 0.5 mM, isoform CA VA, in 20 m M HEPES (pH 7.5), and 20 mM Na2SO4, at 22°C
0.5
-
IC50 above 0.5 mM, isoform CA IX, in 20 m M HEPES (pH 7.5), and 20 mM Na2SO4, at 22°C
0.5
-
IC50 above 0.5 mM, isoform CA III, in 20 m M HEPES (pH 7.5), and 20 mM Na2SO4, at 22°C
0.5
-
IC50 Value (4 results)
COMMENTARY
EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
LITERATURE
ALR1, pH not specified in the publication, temperature not specified in the publication
0.5
-
ALR2, pH not specified in the publication, temperature not specified in the publication
0.4
-
References & Links
Literature References (19)
Links to other databases for coumarin
ChEBI
PubChem
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