Ligand isocitric acid

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Basic Ligand Information

Molecular Structure
Picture of isocitric acid (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C6H8O7
isocitric acid
ODBLHEXUDAPZAU-UHFFFAOYSA-N
Synonyms:
1-hydroxy-1,2,3-propanetricarboxylate, D,L-isocitrate, DL-isocitrate, Ds-isocitrate, iso-citrate, isocitrate, isocitrate analogues

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (17 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
isocitrate + NAD+ = ?
show the reaction diagram
-
caffeoyl-CoA + isocitrate = CoA + 2-caffeoylisocitrate
show the reaction diagram
-

In Vivo Product in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
cis-aconitate + H2O = isocitrate
show the reaction diagram
-
-

Substrate in Enzyme-catalyzed Reactions (42 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
S-adenosyl-L-methionine + DL-isocitrate = S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
DL-isocitrate = ?
show the reaction diagram
-
ATP + DL-isocitrate + CoA = ADP + phosphate + isocitryl-CoA
show the reaction diagram
-

Product in Enzyme-catalyzed Reactions (4 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
succinate + glyoxylate = isocitrate
show the reaction diagram
-
-

Activator in Enzyme-catalyzed Reactions (15 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
25% activation, stimulates NADP+ regeneration
-
oxidation of malate to oxaloacetate
-
isozymes ICDH2 and ICDH1
-
10 M, 112% of initial activity
-
1 mM, relative activity 101%
-
activates isoenzyme Hex II
-
activation
-
1.7fold stimulation only for phlorizin activity
-
activates
-
activation
-

Inhibitor in Enzyme-catalyzed Reactions (45 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
competitive, pH-dependent substrate inhibition
-
reduction of oxaloacetate to malate
-
5 mM
-
competitive inhibition with respect to 2-oxoglutarate
-
10 M, 64% of initial activity
-
inhibits only kinase activity
-
above 5.0 mM, weak
-
L-isocitrate
-
inhibits enzyme form OAT-2
-
weak inhibition
-
noncompetitive, localization of binding pocket on the enzyme outside the active site
-

3D Structure of Enzyme-Ligand-Complex (PDB) (42 results)

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (72 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
1.1
-
pH 8, 25°C, Lineweaver-Burk method

KM Value (246 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE

Ki Value (11 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
TABLE ID
1.5
2.1
calculated from the slopes or intercepts
7114
6.6
-
cytoplasmic enzyme
13659
7.4
-
-
41399

References & Links

Links to other databases for isocitric acid

ChEBI
PubChem
ChEBI
PubChem