Ligand vitamin D3

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Basic Ligand Information

Molecular Structure
Picture of vitamin D3 (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C27H44O
vitamin D3
Synonyms:
calciol, cholecalciferol, vitamin D


Show all pahtways known for Show all BRENDA pathways known for vitamin D3

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (4 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
calciol + O2 + [reduced NADPH-hemoprotein reductase] = calcidiol + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
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cholecalciferol + NADPH + O2 = 25-hydroxyvitamin D3 + NADP+ + H2O
show the reaction diagram
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Substrate in Enzyme-catalyzed Reactions (13 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
cholecalciferol + H2O2 = 25-hydroxycholecalciferol + 24-hydroxycholecalciferol + 26,27-hydroxycholecalciferol + H2O
show the reaction diagram
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cholecalciferol + NADPH + O2 = 25-hydroxyvitamin D3 + NADP+ + H2O
show the reaction diagram
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vitamin D3 + reduced adrenodoxin + O2 = 20-hydroxyvitamin D3 + oxidized adrenodoxin + H2O
show the reaction diagram
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Activator in Enzyme-catalyzed Reactions (4 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
activates oxidative activity in HCT8 and LoVo cells
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0.2 mM, activation to 136% of the control
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aging does not change the induction of enzyme by hormonally active vitamin D3
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Inhibitor in Enzyme-catalyzed Reactions (6 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
equimolar to substrate inhibits 38% of enzyme activity
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inhibits reductive activity in HCT8 and LoVo cells
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decreases parathyroid-induced stimulation of AC6 and induces a phosphorylation of AC6 in a PKC-dependent manner
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22% inhibition at 0.00001 mM, reduces dexamethasone induced increase in enzyme expression
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Enzyme Kinetic Parameters

kcat Value (Turnover Number) (8 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.0023
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25-hydroxylation, mutant R73V/R84A, pH not specified in the publication, temperature not specified in the publication
0.23
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isoenzyme S25DH1, pH 7.0, 30°C

KM Value (12 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE

Ki Value (1 result)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.001
0.002
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References & Links

Links to other databases for vitamin D3

ChEBI
PubChem
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