• BRENDA home

• Basic Ligand Information
• • Molecular Structure
  • Sum Formula
  • BRENDA Name
  • Synonyms
  • InChIKey
  • Pathways
• Roles as Enzyme Ligand
• • In Vivo Substrate
  • In Vivo Product
  • Substrate
  • Product
  • Cofactor
  • Activator
  • Inhibitor
  • Metals and Ions
  • 3D Structure (PDB)
• Enzyme Kinetic Parameters
• • k_cat Value (Turnover Number)
  • K_M Value
  • K_i Value
  • IC₅₀ Value
• References & Links
• • References
  • Links

print visible entries

print all entries

Ligand formyl-CoA

Basic Ligand Information

Molecular Structure

close all tables

open all tables

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (2 results)

top print hide

Show Natural Substrate (2 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.8.3.16

formyl-CoA + oxalate = formate + oxalyl-CoA

739759, 645959, 645960, 645962, 726452, 645961, 645963, 671452, 674225, 692373, 692851, 693216, 693608, 692677

-

3.1.2.10

formyl-CoA + H2O = ?

80970

-

In Vivo Product in Enzyme-catalyzed Reactions (2 results)

top print hide

Show Natural Product (2 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.2.1.10

formaldehyde + CoA + NAD+ = formyl-CoA + NADH + H+

-

-

4.1.1.8

Oxalyl-CoA = Formyl-CoA + CO2

653142

2ji8, 3D-view

Substrate in Enzyme-catalyzed Reactions (3 results)

top print hide

Show Substrate (3 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.8.3.16

formyl-CoA + oxalate = formate + oxalyl-CoA

692851, 693608, 671452, 674225, 692677, 739759, 645959, 645960, 645961, 645962, 645963, 692373, 726452, 662253, 693216

-

3.1.2.10

formyl-CoA + H2O = CoA + formate

80970

-

3.1.2.10

formyl-CoA + H2O = ?

80970

-

3.1.2.10

2 entries

Product in Enzyme-catalyzed Reactions (8 results)

top print hide

Show Product (8 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.2.1.10

formaldehyde + CoA + NAD+ = formyl-CoA + NADH

-

-

1.2.1.10

formaldehyde + CoA + NAD+ = formyl-CoA + NADH + H+

-

-

1.2.1.10

2 entries

1.2.7.11

glyoxylate + CoA + 2 oxidized methyl viologen = formyl-CoA + CO2 + 2 reduced methyl viologen + 2 H+

-

-

1.2.7.7

glyoxylate + CoA + oxidized ferredoxin = formyl-CoA + CO2 + reduced ferredoxin

-

-

2.8.3.18

succinyl-CoA + formate = formyl-CoA + succinate

-

-

2.8.3.9

formate + acetoacetyl-CoA = formyl-CoA + acetoacetate

-

-

4.1.1.8

Oxalyl-CoA = Formyl-CoA + CO2

4838, 4839, 653142

2ji8, 3D-view

6.2.1.1

ATP + formate + CoA = AMP + diphosphate + formyl-CoA

-

-

3D Structure of Enzyme-Ligand-Complex (PDB) (1 result)

top print hide

Show 3D Structure of Enzyme-Ligand-Complex (PDB) (1 entry)

EC NUMBER

ENZYME 3D STRUCTURE

4.1.1.8

2ji8, 3D-view

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (2 results)

top print hide

Show Turnover Number (2 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

2.8.3.16

4.3

-

-

662253

2.8.3.16

4.5

-

in 50 mM potassium phosphate, pH 6.7, at 25°C

726452

2.8.3.16

2 entries

K_M Value (12 results)

top print hide

Show KM Value (12 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.8.3.16

0.002

-

wild-type FRC

692851

2.8.3.16

0.002

-

wild-type formyl-CoA transferase

693216

2.8.3.16

0.0034

-

in 50 mM potassium phosphate, pH 6.7, at 25°C

726452

2.8.3.16

0.0047

-

His-FRC

692851

2.8.3.16

0.0047

-

G259A mutant formyl-CoA transferase

693216

2.8.3.16

0.0058

-

W48Q FRC

692851

2.8.3.16

0.0171

-

W48F FRC

692851

2.8.3.16

0.018

-

G260A mutant formyl-CoA transferase

693216

2.8.3.16

0.033

-

Q17A mutant formyl-CoA transferase

693216

2.8.3.16

0.35

-

His-YfdW

692851

2.8.3.16

3

-

pH 6.7, 25°C

645959

2.8.3.16

11 entries

3.1.2.10

0.045

-

-

80970

References & Links

Literature References (19)

top print hide

Show Reference (19 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

4838

-

Oxalyl-CoA Decarboxylase

1969

Quale, J.R.

Methods Enzymol.

13

369-372

4839

14101969

Carbon assimilation of Pseudomomnas oxalaticus (OX 1)

1963

Quale, J.R.

Biochem. J.

89

492-503

80970

14063284

Formate metabolism. I. Formyl coenzyme A an intermediate in the formate-dependent decomposition of acetyl phosphate in Clostridium kluyveri

1963

Sly, W.S.; Stadtman, E.R.

J. Biol. Chem.

238

2632-2638

645959

2361939

Purification and characterization of formyl-coenzyme A transferase from Oxalobacter formigenes

1990

Baetz, A.L.; Allison, M.J.

J. Bacteriol.

172

3537-3540

645960

-

Localization of oxalyl-coenzyme A decarboxylase, and formyl-coenzyme A transferase in Oxalobacter formigenes cells

1992

Baetz, A.L.; Allison, M.J.

Syst. Appl. Microbiol.

15

167-171

645961

9150242

DNA sequencing and expression of the formyl coenzyme A transferase gene, frc, from, Oxalobacter formigenes

1997

Sidhu, H.; Ogden, S.D.; Lung, H.Y.; Luttge, B.G.; Baetz, A.L.; Peck, A.B.

J. Bacteriol.

179

3378-3381

645962

12832784

Crystallization and preliminary crystallographic analysis of formyl-CoA tranferase from Oxalobacter formigenes

2003

Ricagno, S.; Jonsson, S.; Richards, N.; Lindqvist, Y.

Acta Crystallogr. Sect. D

59

1276-1277

645963

12839984

Formyl-CoA transferase encloses the CoA binding site at the interface of an interlocked dimer

2003

Ricagno, S.; Jonsson, S.; Richards, N.; Lindqvist, Y.

EMBO J.

22

3210-3219

653142

10832966

Oxalate-degrading Enterococcus faecalis

2000

Hokohama, S.; Honma, Y.; Toma, C.; Ogawa, Y.

Microbiol. Immunol.

44

235-240

662253

15213226

Kinetic and mechanistic characterization of the formyl-CoA transferase from Oxalobacter formigenes

2004

Jonsson, S.; Ricagno, S.; Lindqvist, Y.; Richards, N.G.

J. Biol. Chem.

279

36003-36012

671452

16517636

Transcriptional and functional analysis of oxalyl-coenzyme A (CoA) decarboxylase and formyl-CoA transferase genes from Lactobacillus acidophilus

2006

Azcarate-Peril, M.A.; Bruno-Barcena, J.M.; Hassan, H.M.; Klaenhammer, T.R.

Appl. Environ. Microbiol.

72

1891-1899

674225

17953571

Oxalate consumption by lactobacilli: evaluation of oxalyl-CoA decarboxylase and formyl-CoA transferase activity in Lactobacillus acidophilus

2007

Turroni, S.; Vitali, B.; Bendazzoli, C.; Candela, M.; Gotti, R.; Federici, F.; Pirovano, F.; Brigidi, P.

J. Appl. Microbiol.

103

1600-1609

692373

17506826

Lactobacillus gasseri Gasser AM63T degrades oxalate in a multistage continuous culture simulator of the human colonic microbiota

2007

Lewanika, T.R.; Reid, S.J.; Abratt, V.R.; MacFarlane, G.T.; MacFarlane, S.

FEMS Microbiol. Ecol.

61

110-120

692677

17786282

Stable expression of the oxc and frc genes from Oxalobacter formigenes in human embryo kidney 293 cells: implications for gene therapy of hyperoxaluria

2007

Ye, Z.Q.; Kong, D.B.; Chen, Z.Q.; Yao, L.F.; Guo, H.; Yu, X.; Liu, G.L.; Yang, W.M.

Int. J. Mol. Med.

20

521-526

692851

18245280

Differential substrate specificity and kinetic behavior of Escherichia coli YfdW and Oxalobacter formigenes formyl coenzyme A transferase

2008

Toyota, C.G.; Berthold, C.L.; Gruez, A.; Jonsson, S.; Lindqvist, Y.; Cambillau, C.; Richards, N.G.

J. Bacteriol.

190

2556-2564

693216

18162462

Reinvestigation of the catalytic mechanism of formyl-CoA transferase, a class III CoA-transferase

2008

Berthold, C.L.; Toyota, C.G.; Richards, N.G.; Lindqvist, Y.

J. Biol. Chem.

283

6519-6529

693608

18930770

Use of the frc gene as a molecular marker to characterize oxalate-oxidizing bacterial abundance and diversity structure in soil

2008

Khammar, N.; Martin, G.; Ferro, K.; Job, D.; Aragno, M.; Verrecchia, E.

J. Microbiol. Methods

76

120-127

726452

22374910

Formyl-coenzyme A (CoA):oxalate CoA-transferase from the acidophile Acetobacter aceti has a distinctive electrostatic surface and inherent acid stability

2012

Mullins, E.A.; Starks, C.M.; Francois, J.A.; Sael, L.; Kihara, D.; Kappock, T.J.

Protein Sci.

21

686-696

739759

26748805

Diversity and ecology of oxalotrophic bacteria

2016

Herve, V.; Junier, T.; Bindschedler, S.; Verrecchia, E.; Junier, P.

World J. Microbiol. Biotechnol.

32

28

Links to other databases for formyl-CoA

top print hide

Show Links (1 entry)

ChEBI

PubChem