Ligand bovine serum albumin

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Basic Ligand Information

Molecular Formula
BRENDA Name
InChIKey
bovine serum albumin

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
bovine serum albumin + H2O + O2 = ?
show the reaction diagram
-

Substrate in Enzyme-catalyzed Reactions (48 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
bovine serum albumin + H2O + O2 = ?
show the reaction diagram
-
S-adenosyl-L-methionine + bovine serum albumin = S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
L-arginyl-tRNAArg + bovine serum albumin = tRNAArg + L-arginyl-[bovine serum albumin]
show the reaction diagram
-
bovine serum albumin + NAD+ = nicotinamide + ?
show the reaction diagram
-
ATP + bovine serum albumin = ADP + phosphorylated bovine serum albumin
show the reaction diagram
-
ATP + bovine serum albumin = ADP + [bovine serum albumin] phosphate
show the reaction diagram
-
bovine serum albumin + H2O = ? + phosphate
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
Bovine serum albumin + H2O = Hydrolyzed bovine serum albumin
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
Bovine serum albumin + H2O = Hydrolyzed bovine serum albumin
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
bovine serum albumin + H2O = fragments of bovine serum albumin
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
Bovine serum albumin + H2O = Hydrolyzed bovine serum albumin
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-
Bovine serum albumin + H2O = ?
show the reaction diagram
-

Activator in Enzyme-catalyzed Reactions (148 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
final concentration of 0.05% stimulates 50%
-
10-15% increased activity
-
activity increased
-
addition to reaction mixture enhances luciferase activity
-
2 mg/ml, 30% activation of purified enzyme, stimulation by bovine serum albumin and catalase is additive and less pronounced in crude cell extract
-
2 mg7ml, 930% activation
-
stimulation
-
stimulates the recombinant enzyme by 41% at 0.1 mM
-
1-10 mg/ml, stimulates enzyme activity of unwashed microsomes by 50%
-
stimulates
-
twofold increase of enzyme activity
-
2 mg per ml increases activity by more than 5 times
-
activates 3fold at 1 mg/ml
-
slightly increases ACO activity, maximum value of ACO acitvity at 0.036 mM
-
time-dependent increases in NAD(P)H oxidase activity with bovine serum albumin stimulation that is inhibited in a concentration-dependent manner with the HMG-CoA reductase inhibitor rosuvastatin, or with Rac1 inhibitor NSC23766. Following albumin stimulation, Rac1 translocates to plasma membrane for NAD(P)H oxidase activation
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2 mg/ml, activation
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2 mg/ml, activation
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activation, molar ratio substrate linoleoyl-CoA/albumin: 2:4:1
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activation, by inhibiting the formation of palmitoyl-CoA micelles
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induces T-lymphocyte enzyme activity in the presence of palmitoyl-CoA but not oleoyl-CoA
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fatty acid deficient, 2 mg per ml
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optimal concentration of 1-1.5 mg/ml at short incubation periods and 2-2.5 mg bovine serum albumin/ml during longer incubation periods
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stimulates
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defatted, 2fold activation in crude enzyme extract at concentration below 5 mg/ml, inhibition above
-
activates isozyme UGT1A9 by reducing the Km of the substrates, overview
-
enhances activity by stabilizing TCH4 protein conformation
-
addition to the assay system causes 2.5fold higher reaction rate
-
enhances activity
-
necessary in homogenization media
-
50% activation of pure enzyme
-
stimulates
-
activates
-
activates recombinant enzyme
-
stimulates
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0.0025%, increases activity of purified enzyme by 40%
-
activation at 0.2 mg/ml
-
activation
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the enyzme activity is higher with substrate solubilized by bovine serum albumin compared to Triton X-100
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activation, can substitute for histone only in the absence of phosphate
-
0.01 mg/ml, stimulates
-
activity is 6fold increased
-
stimulates
-
DNA polymerase is stabilized with an approximately 25% increase in enzyme activity
-
1 mg/ml, 2fold increase of activity
-
specific activity increases in presence of 0.5-2 mg/ml bovine serum albumin, microsomal fraction of human mesangial cells
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MAGL activity increases with increasing concentration up to 1%
-
activity is affected considerably, 5.0 mg/ml prevents substrate polymerization
-
0.01 mg/reaction volume stimulates about 50% at both pH 6.2 and 7.7
-
130% activity at 1% at pH 8.0 and 37°C
-
10 mg/ml, 30°C, pH 7.5, 112% relative activity with histone as substrate
-
stimulates only in urine at pH 3.9
-
0.125 mg/l, 35% activation
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activation
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assists reactivation after unfolding
-
increases acitivity up to 100%
-
1 mg/ml, activates
-
10% (w/v) bovine serum albumin improves the hydrolytic rate by a factor of 2
-
slightly enhances activity
-
addition of bovine serum albumin slightly increases activity
-
activation, 0.5%: 180% activity
-
activation
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activation
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1 mg/ml stimulates activity
-
stimulation
-
optimal activity at 0.25 mg/ml
-
activation
-
stimulates
-
0.025 mg/ml, stimulating
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Inhibitor in Enzyme-catalyzed Reactions (33 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
inhibition when present in large excess
-
above 6 mg/ml, at 0.01 mg palmitoyl-CoA depending on palmitoyl-CoA and phospholipid concentration
-
3 mg/ml, 50% inhibition
-
inhibits at high concentrations
-
weak
-
above 5 mg/ml
-
no influence on enzyme activity
-
slight inhibition
-
amphipathic protein, the inhibition is fully reversible by addition of bile salts and colipase
-
BSA forms a weak conjugate with the substrate which inhibits the enzyme competitively
-
90% inhibition at 0.015 mg/ml, addition of inhibitor changes pH-activity curve, optimal pH shifted to the alkaline side, DNA substrate compete with inhibitor and reverses inhibitor action
-
1 mg/ml 30°C, pH 7.5, 95% remaining activity with glycogen synthase D and 92% with phosphorylase, in presence of 10 mg/ml 79% remaining activity with phosphorylase a as substrate
-
concentration-dependent inhibition on lysoPLD activity in egg white during 24 h incubation
-
38% inhibition at 0.01%
-
5 mg, 45% inhibition
-

Enzyme Kinetic Parameters

KM Value (3 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.24
-
-

References & Links

Links to other databases for bovine serum albumin